| Microcalorimetric investigation of the toxic action of Cr(VI) on the metabolism of Tetrahymena thermophila BF5 during growth | |
| Zheng, Dan; Liu, Yi; Zhang, Yue; Chen, Xiao-Juan; Shen, Yun-Fen; Liu, Y, Wuhan Univ, Coll Chem & Mol Sci, Dept Biol Chem, Wuhan 430072, Peoples R China | |
| 2006-09-01 | |
| Source Publication | ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
 |
| ISSN | 1382-6689 |
| Volume | 22Issue:2Pages:121-127 |
| Abstract | Tetrahymena thermophila BF5 produce heat by metabolism and movement. Using a TAM air isothermal microcalorimeter, the power-time curves of the metabolism of T thermophila BF5 during growth were obtained and the action on them by the addition of Cr(VI) were studied. The morphological change with Cr(VI) coexisted and biomass change during the process of T thermophila BF5 growth were studied by light microscope. Chromium has been regarded as an essential trace element for life. However, hexavalent chromium is a known carcinogen, mutagen, cytotoxicant and strong oxidizing agent. Cr(VI) of different concentration have different effects on T thermophila BF5 growth with the phenomenon of low dose stimulation (0-3 x 10(-5) mol L-1) and high dose inhibition (3 x 10(-5) to 2.4 x 10(-4) mol L-1). The relationship between the growth rate constant (k) and c is a typical U-shaped curve, which is a characteristic of hormesis. T thermophila BF5 cannot grow at all when the concentration of Cr(VI) is up to 2.4 x 10(-4) mol L-1. The microscopic observations agree well with the results obtained by means of microcalorimetry. And T thermophila BF5 had obviously morphological changes by the addition of Cr(VI). (c) 2006 Elsevier B.V. All rights reserved.; Tetrahymena thermophila BF5 produce heat by metabolism and movement. Using a TAM air isothermal microcalorimeter, the power-time curves of the metabolism of T thermophila BF5 during growth were obtained and the action on them by the addition of Cr(VI) were studied. The morphological change with Cr(VI) coexisted and biomass change during the process of T thermophila BF5 growth were studied by light microscope. Chromium has been regarded as an essential trace element for life. However, hexavalent chromium is a known carcinogen, mutagen, cytotoxicant and strong oxidizing agent. Cr(VI) of different concentration have different effects on T thermophila BF5 growth with the phenomenon of low dose stimulation (0-3 x 10(-5) mol L-1) and high dose inhibition (3 x 10(-5) to 2.4 x 10(-4) mol L-1). The relationship between the growth rate constant (k) and c is a typical U-shaped curve, which is a characteristic of hormesis. T thermophila BF5 cannot grow at all when the concentration of Cr(VI) is up to 2.4 x 10(-4) mol L-1. The microscopic observations agree well with the results obtained by means of microcalorimetry. And T thermophila BF5 had obviously morphological changes by the addition of Cr(VI). (c) 2006 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | t Thermophila Bf5 Microcalorimetry Morphological Change Toxicology Growth Metabolism |
| Department | Wuhan Univ, Coll Chem & Mol Sci, Dept Biol Chem, Wuhan 430072, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Subject Area | Environmental Sciences ; Pharmacology & Pharmacy ; Toxicology |
| DOI | 10.1016/j.etap.2005.12.011 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology |
| WOS Subject | Environmental Sciences ; Pharmacology & Pharmacy ; Toxicology |
| WOS ID | WOS:000239683900001 |
| WOS Keyword | IN-VITRO ; PYRIFORMIS ; CHROMIUM ; CHROMATE ; HORMESIS ; CYTOTOXICITY ; BIOASSAY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8884 |
| Collection | 期刊论文 |
| Corresponding Author | Liu, Y, Wuhan Univ, Coll Chem & Mol Sci, Dept Biol Chem, Wuhan 430072, Peoples R China |
| Affiliation | 1.Wuhan Univ, Coll Chem & Mol Sci, Dept Biol Chem, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zheng, Dan,Liu, Yi,Zhang, Yue,et al. Microcalorimetric investigation of the toxic action of Cr(VI) on the metabolism of Tetrahymena thermophila BF5 during growth[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2006,22(2):121-127. |
| APA | Zheng, Dan,Liu, Yi,Zhang, Yue,Chen, Xiao-Juan,Shen, Yun-Fen,&Liu, Y, Wuhan Univ, Coll Chem & Mol Sci, Dept Biol Chem, Wuhan 430072, Peoples R China.(2006).Microcalorimetric investigation of the toxic action of Cr(VI) on the metabolism of Tetrahymena thermophila BF5 during growth.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,22(2),121-127. |
| MLA | Zheng, Dan,et al."Microcalorimetric investigation of the toxic action of Cr(VI) on the metabolism of Tetrahymena thermophila BF5 during growth".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 22.2(2006):121-127. |
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