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学科主题: Environmental Sciences; Marine & Freshwater Biology
题名: Nitrate and phosphate supplementation to increase toxin production by the marine dinoflagellate Alexandrium tamarense
作者: Hu, Hanhua; Chen, Weidong; Shi, Yanjun; Cong, Wei
通讯作者: Hu, HH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: Alexandrium tamarense ; growth ; nitrate ; phosphate ; saxitoxin
刊名: MARINE POLLUTION BULLETIN
发表日期: 2006-07-01
DOI: 10.1016/j.marpolbul.2005.11.005
卷: 52, 期:7, 页:756-760
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Univ Petr Beijing, Fac Chem Sci & Engn, Dept Biochem Engn, Beijing 102249, Peoples R China; Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences ; Marine & Freshwater Biology
研究领域[WOS]: Environmental Sciences & Ecology ; Marine & Freshwater Biology
摘要: Alexandrium tamarense toxins have great value in biotechnology research as well as important in connection with shellfish poisoning. The influence of nitrate or nitrate and phosphate supplementation on cell biomass and toxin content were investigated in batch cultures. When cultures at low nitrate (88.2 mu M NaNO3) Were supplemented with 793.8 mu M NaNO3 at day 10 the cell density and cellular toxin contents were increased by 6-29% and 20-76%, respectively, compared with controls, and maximal values were 43,600 cells/ml (day 38) and 0.91 pg/cell (day 31). Supplementation with nitrate at day 14 or with nitrate and phosphate at day 10/14 to the cultures did not increase the cell density compared with the non-supplemented middle nitrate or high phosphate (108 mu M NaH2PO4) cultures, respectively, but increased the cellular toxin contents by an average of 52%. The results showed that supplementation with nitrate or with nitrate and phosphate at different growth phases of the cultures increased toxin yield by an average of 46%. Supplementation with nitrate at selected times to maintain continuous low level of nitrate might contribute to the effective increase of toxin yield of A. tamarense. (c) 2005 Elsevier Ltd. All rights reserved.
英文摘要: Alexandrium tamarense toxins have great value in biotechnology research as well as important in connection with shellfish poisoning. The influence of nitrate or nitrate and phosphate supplementation on cell biomass and toxin content were investigated in batch cultures. When cultures at low nitrate (88.2 mu M NaNO3) Were supplemented with 793.8 mu M NaNO3 at day 10 the cell density and cellular toxin contents were increased by 6-29% and 20-76%, respectively, compared with controls, and maximal values were 43,600 cells/ml (day 38) and 0.91 pg/cell (day 31). Supplementation with nitrate at day 14 or with nitrate and phosphate at day 10/14 to the cultures did not increase the cell density compared with the non-supplemented middle nitrate or high phosphate (108 mu M NaH2PO4) cultures, respectively, but increased the cellular toxin contents by an average of 52%. The results showed that supplementation with nitrate or with nitrate and phosphate at different growth phases of the cultures increased toxin yield by an average of 46%. Supplementation with nitrate at selected times to maintain continuous low level of nitrate might contribute to the effective increase of toxin yield of A. tamarense. (c) 2005 Elsevier Ltd. All rights reserved.
关键词[WOS]: PHOSPHORUS LIMITATION ; NATURAL PHYTOPLANKTON ; C2 TOXIN ; GROWTH ; SAXITOXIN ; TOXICITY ; DYNAMICS ; NITROGEN ; CULTURES ; COASTAL
语种: 英语
WOS记录号: WOS:000239854400013
ISSN号: 0025-326X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8870
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Petr Beijing, Fac Chem Sci & Engn, Dept Biochem Engn, Beijing 102249, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China

Recommended Citation:
Hu, Hanhua; Chen, Weidong; Shi, Yanjun; Cong, Wei.Nitrate and phosphate supplementation to increase toxin production by the marine dinoflagellate Alexandrium tamarense,MARINE POLLUTION BULLETIN,2006,52(7):756-760
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