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Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera)
Zhao, Xian-Fu; Liu, Guo-Xiang; Hu, Zheng-Yu; Hu, ZY, Chinese Acad Sci, Inst Water Engn Ecol, Minist Water Resources, XiongChu Rd 578, Wuhan 430079, Hubei, Peoples R China
2006-06-01
Source PublicationCRUSTACEANA
ISSN0011-216X
Volume79Issue:6Pages:677-689
AbstractOur studies investigated the physico-chemical properties of alkaline phosphatase excreted by D. magna. This cladoceran mainly released alkaline phosphatase, though it also released a small amount of acid phosphatase. The alkaline phosphatase showed a broad pH optimum (8.05-10.0), and had a broad optimum temperature (30-35 degrees C) with a temperature coefficient (Q(10)) of 2.45. The K-m of the enzyme is 0.15 +/- 0.02 mM when p-nitrophenyl phosphate is used as a substrate, and the V-max is 0.43 +/- 0.01 mu M pNP mg(-1) DW h(-1). Even though alkaline phosphatase had been incubated in chloroform saturated with WC medium for 13 days, its activity was 54% that of the original. The enzyme was strongly inactivated by EDTA, and appeared to be zinc dependent. The alkaline phosphatase activity remained constant when D. magna was fed different quantities of Chlorella sp. The sensitivity of D. magna phosphatase activity to phosphate was time-dependent. During the first 16 hrs, the enzyme was insensitive to phosphate addition, after 24 hrs incubation the enzyme became sensitive to phosphate addition.; Our studies investigated the physico-chemical properties of alkaline phosphatase excreted by D. magna. This cladoceran mainly released alkaline phosphatase, though it also released a small amount of acid phosphatase. The alkaline phosphatase showed a broad pH optimum (8.05-10.0), and had a broad optimum temperature (30-35 degrees C) with a temperature coefficient (Q(10)) of 2.45. The K-m of the enzyme is 0.15 +/- 0.02 mM when p-nitrophenyl phosphate is used as a substrate, and the V-max is 0.43 +/- 0.01 mu M pNP mg(-1) DW h(-1). Even though alkaline phosphatase had been incubated in chloroform saturated with WC medium for 13 days, its activity was 54% that of the original. The enzyme was strongly inactivated by EDTA, and appeared to be zinc dependent. The alkaline phosphatase activity remained constant when D. magna was fed different quantities of Chlorella sp. The sensitivity of D. magna phosphatase activity to phosphate was time-dependent. During the first 16 hrs, the enzyme was insensitive to phosphate addition, after 24 hrs incubation the enzyme became sensitive to phosphate addition.
SubtypeArticle
KeywordPhosphorus-deficiency Lake Phytoplankton Zooplankton Algae Indicators Water Food
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaMarine & Freshwater Biology
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000240232900004
WOS KeywordPHOSPHORUS-DEFICIENCY ; LAKE ; PHYTOPLANKTON ; ZOOPLANKTON ; ALGAE ; INDICATORS ; WATER ; FOOD
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8852
Collection期刊论文
Corresponding AuthorHu, ZY, Chinese Acad Sci, Inst Water Engn Ecol, Minist Water Resources, XiongChu Rd 578, Wuhan 430079, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Xian-Fu,Liu, Guo-Xiang,Hu, Zheng-Yu,et al. Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera)[J]. CRUSTACEANA,2006,79(6):677-689.
APA Zhao, Xian-Fu,Liu, Guo-Xiang,Hu, Zheng-Yu,&Hu, ZY, Chinese Acad Sci, Inst Water Engn Ecol, Minist Water Resources, XiongChu Rd 578, Wuhan 430079, Hubei, Peoples R China.(2006).Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera).CRUSTACEANA,79(6),677-689.
MLA Zhao, Xian-Fu,et al."Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera)".CRUSTACEANA 79.6(2006):677-689.
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