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Gene structure and transcription of IRF-2 in the mandarin fish Siniperca chuatsi with the finding of alternative transcripts and microsatellite in the coding region
Sun, Baojian; Chang, Mingxian; Chen, Dali; Nie, Pin; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2006-09-01
Source PublicationIMMUNOGENETICS
ISSN0093-7711
Volume58Issue:9Pages:774-784
AbstractThe gene of interferon regulatory factor-2 (IRF-2) has been cloned from the mandarin fish (Siniperca chuatsi). The IRF-2 gene has 6,418 nucleotides (nt) and contains eight exons and seven introns, encoding two mRNAs. The two IRF-2 mRNAs each contained an open reading frame of 873 nt, which both translate into the same 291 amino acids but differed in their 5' untranslated region: one mRNA was transcribed initially from the exon 1 bypassing exon 2, while the other was transcribed from the exon 2. The microsatellites (CA repeats) could be found in the carboxyl terminal region of mandarin fish IRF-2, which result in the truncated form molecules. The microsatellites' polymorphism was investigated, and eight alleles were found in 16 individuals. The microsatellites were also examined in IRF-2 of several freshwater perciform fishes. The transcription of the IRF-2 in different tissues with or without poly inosine-cytidine stimulation was analyzed by real-time PCR, and the constitutive transcription of both molecules could be detected in all the tissues examined.; The gene of interferon regulatory factor-2 (IRF-2) has been cloned from the mandarin fish (Siniperca chuatsi). The IRF-2 gene has 6,418 nucleotides (nt) and contains eight exons and seven introns, encoding two mRNAs. The two IRF-2 mRNAs each contained an open reading frame of 873 nt, which both translate into the same 291 amino acids but differed in their 5' untranslated region: one mRNA was transcribed initially from the exon 1 bypassing exon 2, while the other was transcribed from the exon 2. The microsatellites (CA repeats) could be found in the carboxyl terminal region of mandarin fish IRF-2, which result in the truncated form molecules. The microsatellites' polymorphism was investigated, and eight alleles were found in 16 individuals. The microsatellites were also examined in IRF-2 of several freshwater perciform fishes. The transcription of the IRF-2 in different tissues with or without poly inosine-cytidine stimulation was analyzed by real-time PCR, and the constitutive transcription of both molecules could be detected in all the tissues examined.
SubtypeArticle
KeywordIrf-2 Gene Organization Microsatellite Mandarin Fish Chinese Perch Siniperca Chuatsi
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Subject AreaGenetics & Heredity ; Immunology
DOI10.1007/s00251-006-0129-y
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity ; Immunology
WOS SubjectGenetics & Heredity ; Immunology
WOS IDWOS:000240317200007
WOS KeywordINTERFERON REGULATORY FACTOR ; TROUT ONCORHYNCHUS-MYKISS ; MOLECULAR-CLONING ; FACTOR-1 IRF-1 ; FUGU-RUBRIPES ; CELL-CYCLE ; EXPRESSION ; ACTIVATION ; PROMOTER ; DNA
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Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8848
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Sun, Baojian,Chang, Mingxian,Chen, Dali,et al. Gene structure and transcription of IRF-2 in the mandarin fish Siniperca chuatsi with the finding of alternative transcripts and microsatellite in the coding region[J]. IMMUNOGENETICS,2006,58(9):774-784.
APA Sun, Baojian,Chang, Mingxian,Chen, Dali,Nie, Pin,&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.(2006).Gene structure and transcription of IRF-2 in the mandarin fish Siniperca chuatsi with the finding of alternative transcripts and microsatellite in the coding region.IMMUNOGENETICS,58(9),774-784.
MLA Sun, Baojian,et al."Gene structure and transcription of IRF-2 in the mandarin fish Siniperca chuatsi with the finding of alternative transcripts and microsatellite in the coding region".IMMUNOGENETICS 58.9(2006):774-784.
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