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Population genetic structure of Carchesium polypinum (Ciliophora : Peritrichia) in four chinese lakes inferred from ISSR fingerprinting: High diversity but low differentiation
Zhang, Wen-Jing; Yang, Jun; Yu, Yu-He; Shu, Shao-Wu; Shen, Yun-Fen; Yu, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater & Biotechnol, Wuhan 430072, Peoples R China
2006-09-01
Source PublicationJOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN1066-5234
Volume53Issue:5Pages:358-363
AbstractAlthough the peritrichous ciliate Carchesium polypinum is common in freshwater, its population genetic structure is largely unknown. We used inter-simple sequence repeat (ISSR) fingerprinting to analyze the genetic structure of 48 different isolates of the species from four lakes in Wuhan, central China. Using eight polymorphic primers, 81 discernible DNA fragments were detected, among which 76 (93.83%) were polymorphic, indicating high genetic diversity at the isolate level. Further, Nei's gene diversity (h) and Shannon's Information index (I) between the different isolates both revealed a remarkable genetic diversity, higher than previously indicated by their morphology. At the same time, substantial gene flow was found. So the main factors responsible for the high level of diversity within populations are probably due to conjugation (sexual reproduction) and wide distribution of swarmers. Analysis of molecular variance (AMOVA) showed that there was low genetic differentiation among the four populations probably due to common ancestry and flooding events. The cluster analysis and principal component analysis (PCA) suggested that genotypes isolated from the same lake displayed a higher genetic similarity than those from different lakes. Both analyses separated C. polypinum isolates into subgroups according to the geographical locations. However, there is only a weak positive correlation between the genetic distance and geographical distance, suggesting a minor effect of geographical distance on the distribution of genetic diversity between populations of C. polypinum at the local level. In conclusion, our studies clearly demonstrated that a single morphospecies may harbor high levels of genetic diversity, and that the degree of resolution offered by morphology as a marker for measuring distribution patterns of genetically distinct entities is too low.; Although the peritrichous ciliate Carchesium polypinum is common in freshwater, its population genetic structure is largely unknown. We used inter-simple sequence repeat (ISSR) fingerprinting to analyze the genetic structure of 48 different isolates of the species from four lakes in Wuhan, central China. Using eight polymorphic primers, 81 discernible DNA fragments were detected, among which 76 (93.83%) were polymorphic, indicating high genetic diversity at the isolate level. Further, Nei's gene diversity (h) and Shannon's Information index (I) between the different isolates both revealed a remarkable genetic diversity, higher than previously indicated by their morphology. At the same time, substantial gene flow was found. So the main factors responsible for the high level of diversity within populations are probably due to conjugation (sexual reproduction) and wide distribution of swarmers. Analysis of molecular variance (AMOVA) showed that there was low genetic differentiation among the four populations probably due to common ancestry and flooding events. The cluster analysis and principal component analysis (PCA) suggested that genotypes isolated from the same lake displayed a higher genetic similarity than those from different lakes. Both analyses separated C. polypinum isolates into subgroups according to the geographical locations. However, there is only a weak positive correlation between the genetic distance and geographical distance, suggesting a minor effect of geographical distance on the distribution of genetic diversity between populations of C. polypinum at the local level. In conclusion, our studies clearly demonstrated that a single morphospecies may harbor high levels of genetic diversity, and that the degree of resolution offered by morphology as a marker for measuring distribution patterns of genetically distinct entities is too low.
SubtypeArticle
KeywordBiodiversity Gene Flow Genetic Differentiation Genetic Diversity Peritrichous Ciliate
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater & Biotechnol, Wuhan 430072, Peoples R China; China Univ Geosci, Sch Environm Studies, Wuhan 430074, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaMicrobiology
DOI10.1111/j.1550-7408.2006.00113.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000240404500006
WOS KeywordPOLYMORPHIC DNA RAPD ; TRYPANOSOMA-CRUZI ; MARKERS ; BIODIVERSITY ; DISPERSAL ; GENOTYPES ; DISEASE
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8844
Collection期刊论文
Corresponding AuthorYu, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater & Biotechnol, Wuhan 430072, Peoples R China
2.China Univ Geosci, Sch Environm Studies, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Wen-Jing,Yang, Jun,Yu, Yu-He,et al. Population genetic structure of Carchesium polypinum (Ciliophora : Peritrichia) in four chinese lakes inferred from ISSR fingerprinting: High diversity but low differentiation[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2006,53(5):358-363.
APA Zhang, Wen-Jing,Yang, Jun,Yu, Yu-He,Shu, Shao-Wu,Shen, Yun-Fen,&Yu, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater & Biotechnol, Wuhan 430072, Peoples R China.(2006).Population genetic structure of Carchesium polypinum (Ciliophora : Peritrichia) in four chinese lakes inferred from ISSR fingerprinting: High diversity but low differentiation.JOURNAL OF EUKARYOTIC MICROBIOLOGY,53(5),358-363.
MLA Zhang, Wen-Jing,et al."Population genetic structure of Carchesium polypinum (Ciliophora : Peritrichia) in four chinese lakes inferred from ISSR fingerprinting: High diversity but low differentiation".JOURNAL OF EUKARYOTIC MICROBIOLOGY 53.5(2006):358-363.
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