| Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase | |
| Zhu, Rong; Zhang, Yi-Bing; Chen, Yu-Dong; Dong, Cai-Wen; Zhang, Fu-Tie; Zhang, Qi-Ya; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Chinese Acad Sci, Inst Hydrobiol,State Key Lab Freshwater Ecol & Bi, Wuhan 430072, Peoples R China | |
| 2006 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| Volume | 30Issue:11Pages:1047-1059 |
| Abstract | The heme-regulated initiation factor 2 alpha kinase (HRI) is acknowledged to play an important role in translational shutoff in reticulocytes in response to various cellular stresses. In this study, we report its homologous cDNA cloning and characterization from cultured flounder embryonic cells (FEC) after treatment with UV-inactivated grass carp haemorrhagic virus (GCHV). The full-length cDNA of Paralichthys olivaceus HRI homologue (PoHRI) has 2391 bp and encodes a protein of 651 amino acids. The putative PoHRI protein exhibits high identity with all members of eIF2 alpha kinase family. It contains 12 catalytic subdomains located within the C-terminus of all Ser/Thr protein kinases, a unique kinase insertion of 136 amino acids between subdomains IV and V, and a relatively conserved N-terminal domain (NTD). Upon heat shock, virus infection or Poly PC treatment, PoHRI mRNA and protein are significantly upregulated in FEC cells but show different expression patterns in response to different stresses. In healthy flounders, PoHRI displays a wide tissue distribution at both the mRNA and protein levels. These results indicate that PoHRI is a ubiquitous eIF2a kinase and might play an important role in translational control over nonheme producing FEC cells under different stresses. (c) 2006 Elsevier Ltd. All rights reserved. |
| Keyword | Heme-regulated Initiation Factor 2 Alpha Kinase (Hri) Grass Carp Haemorrhagic Virus (Gchv) Flounder (Paralichthys Olivaceus) Embryonic Cells (Fec) Inductive Expression Stress Responses |
| Department | Chinese Acad Sci, Grad Sch, Chinese Acad Sci, Inst Hydrobiol,State Key Lab Freshwater Ecol & Bi, Wuhan 430072, Peoples R China |
| Subject Area | Immunology ; Zoology |
| Indexed By | SCI |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8830 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, Grad Sch, Chinese Acad Sci, Inst Hydrobiol,State Key Lab Freshwater Ecol & Bi, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, Rong,Zhang, Yi-Bing,Chen, Yu-Dong,et al. Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2006,30(11):1047-1059. |
| APA | Zhu, Rong.,Zhang, Yi-Bing.,Chen, Yu-Dong.,Dong, Cai-Wen.,Zhang, Fu-Tie.,...&Gui, JF, Chinese Acad Sci, Grad Sch, Chinese Acad Sci, Inst Hydrobiol,State Key Lab Freshwater Ecol & Bi, Wuhan 430072, Peoples R China.(2006).Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,30(11),1047-1059. |
| MLA | Zhu, Rong,et al."Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 30.11(2006):1047-1059. |
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