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学科主题: Engineering ; Chemical
题名: Analysis of steric mass-action model for protein adsorption equilibrium onto porous anion-exchange adsorbent
作者: Chen, Wei-Dong; Hu, Han-Hua; Wang, Yan-Dong
通讯作者: Chen, WD, China Univ Petr Beijing, Fac Chem Sci & Engn, Dept Biochem Engn, Beijing 102249, Peoples R China
关键词: ion exchange ; adsorption ; isothermal ; protein ; simulation ; separations
刊名: CHEMICAL ENGINEERING SCIENCE
发表日期: 2006-11-01
DOI: 10.1016/j.ces.2006.07.036
卷: 61, 期:21, 页:7068-7076
收录类别: SCI
文章类型: Article
部门归属: China Univ Petr Beijing, Fac Chem Sci & Engn, Dept Biochem Engn, Beijing 102249, Peoples R China; Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300072, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Technology
类目[WOS]: Engineering, Chemical
研究领域[WOS]: Engineering
摘要: The ion-exchange equilibrium of bovine serum albumin (BSA) to an anion exchanger, DEAE Spherodex M, has been studied by batch adsorption experiments at pH values ranging from 5.26 to 7.6 and ionic strengths from 10 to 117.1 mmol/l. Using the unadjustable adsorption equilibrium parameters obtained from batch experiments, the applicability of the steric mass-action (SMA) model was analyzed for describing protein ion-exchange equilibrium in different buffer systems. The parametric sensitivity analysis was performed by perturbing each of the model parameters, while holding the rest constant. The simulation results showed that, at high salt concentrations or low pHs close to the isoelectric point of the protein, the precision of the model prediction decreased. Parametric sensitivity analysis showed that the characteristic charge and protein steric factor had the largest effects on ion-exchange equilibrium, while the effect of equilibrium constant was about 70%-95% smaller than those of characteristic charge and steric factor under all conditions investigated. The SMA model with the relationship between the adjusted characteristic charge and the salt concentration can well predict the protein adsorption isotherms in a wide pH range from 5.84 to 7.6. It is considered that the SMA model could be further improved by taking into account the effect of salt concentration on the intermolecular interactions of proteins. (c) 2006 Elsevier Ltd. All rights reserved.
英文摘要: The ion-exchange equilibrium of bovine serum albumin (BSA) to an anion exchanger, DEAE Spherodex M, has been studied by batch adsorption experiments at pH values ranging from 5.26 to 7.6 and ionic strengths from 10 to 117.1 mmol/l. Using the unadjustable adsorption equilibrium parameters obtained from batch experiments, the applicability of the steric mass-action (SMA) model was analyzed for describing protein ion-exchange equilibrium in different buffer systems. The parametric sensitivity analysis was performed by perturbing each of the model parameters, while holding the rest constant. The simulation results showed that, at high salt concentrations or low pHs close to the isoelectric point of the protein, the precision of the model prediction decreased. Parametric sensitivity analysis showed that the characteristic charge and protein steric factor had the largest effects on ion-exchange equilibrium, while the effect of equilibrium constant was about 70%-95% smaller than those of characteristic charge and steric factor under all conditions investigated. The SMA model with the relationship between the adjusted characteristic charge and the salt concentration can well predict the protein adsorption isotherms in a wide pH range from 5.84 to 7.6. It is considered that the SMA model could be further improved by taking into account the effect of salt concentration on the intermolecular interactions of proteins. (c) 2006 Elsevier Ltd. All rights reserved.
关键词[WOS]: BOVINE SERUM-ALBUMIN ; ION-EXCHANGE ; AQUEOUS-SOLUTIONS ; CHROMATOGRAPHY ; RETENTION ; DIFFUSION ; KINETICS ; OPTIMIZATION ; SEPARATIONS ; SIMULATION
语种: 英语
WOS记录号: WOS:000241446700017
ISSN号: 0009-2509
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8814
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.China Univ Petr Beijing, Fac Chem Sci & Engn, Dept Biochem Engn, Beijing 102249, Peoples R China
2.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300072, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Chen, Wei-Dong; Hu, Han-Hua; Wang, Yan-Dong.Analysis of steric mass-action model for protein adsorption equilibrium onto porous anion-exchange adsorbent,CHEMICAL ENGINEERING SCIENCE,2006,61(21):7068-7076
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