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Analysis of estrogens in environmental waters using polymer monolith in-polyether ether ketone tube solid-phase microextraction combined with high-performance liquid chromatography
Wen, Yi; Zhou, Bing-Sheng; Xu, Ying; Jin, Shi-Wei; Feng, Yu-Qi; Feng, YQ, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2006-11-10
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1133Issue:1-2Pages:21-28
AbstractA simple, rapid and sensitive on-line method for simultaneous determination of four endocrine disruptors (17 beta-estradiol, estriol, bisphenol A and 17 alpha-ethinylestradiol) in environmental waters was developed by coupling in-tube solid-phase microextraction (SPME) to high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). A poly(acrylamide-vinylpyridine-NAP-methylene bisacrylamide) monolith, synthesized inside a polyether ether ketone (PEEK) tube, was selected as the extraction medium. To achieve optimum extraction performance, several parameters were investigated, including extraction flow-rate, extraction time, and pH value, inorganic salt and organic solvent content of the sample matrix. By simply filtered with nylon membrane filter and adjusting the pH of samples to 6.0 with phosphoric acid, the sample solution then could be directly injected into the device for extraction. Low detection limits (S/N = 3) and quantification limits (S/N = 10) of the proposed method were achieved in the range of 0.006-0.10 ng/mL and 0.02-0.35 ng/mL from spiked lake waters, respectively. The calibration curves of four endocrine disruptors showed good linearity ranging from quantification limits to 50 ng/mL with a linear coefficient R-2 value above 0.9913. Good method reproducibility was also found by intra- and inter-day precisions, yielding the RSDs less than 12 and 9.8%, respectively. Finally, the proposed method was successfully applied to the determination of these compounds in several environmental waters. (c) 2006 Elsevier B.V. All rights reserved.; A simple, rapid and sensitive on-line method for simultaneous determination of four endocrine disruptors (17 beta-estradiol, estriol, bisphenol A and 17 alpha-ethinylestradiol) in environmental waters was developed by coupling in-tube solid-phase microextraction (SPME) to high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). A poly(acrylamide-vinylpyridine-NAP-methylene bisacrylamide) monolith, synthesized inside a polyether ether ketone (PEEK) tube, was selected as the extraction medium. To achieve optimum extraction performance, several parameters were investigated, including extraction flow-rate, extraction time, and pH value, inorganic salt and organic solvent content of the sample matrix. By simply filtered with nylon membrane filter and adjusting the pH of samples to 6.0 with phosphoric acid, the sample solution then could be directly injected into the device for extraction. Low detection limits (S/N = 3) and quantification limits (S/N = 10) of the proposed method were achieved in the range of 0.006-0.10 ng/mL and 0.02-0.35 ng/mL from spiked lake waters, respectively. The calibration curves of four endocrine disruptors showed good linearity ranging from quantification limits to 50 ng/mL with a linear coefficient R-2 value above 0.9913. Good method reproducibility was also found by intra- and inter-day precisions, yielding the RSDs less than 12 and 9.8%, respectively. Finally, the proposed method was successfully applied to the determination of these compounds in several environmental waters. (c) 2006 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordEndocrine Disruptors Environmental Water Polymer Monolith In-polyEther Ether Ketone Tube Solid-phase Microextraction Poly(Acrylamide-vinylpyridine-n,n ''-methylene Bisacrylamide) Monolith Hplc
DepartmentWuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
DOI10.1016/j.chroma.2006.08.049
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000241772800003
WOS KeywordTANDEM MASS-SPECTROMETRY ; BAR SORPTIVE EXTRACTION ; ENDOCRINE DISRUPTING CHEMICALS ; ONLINE PRETREATMENT DEVICE ; PERSONAL-CARE PRODUCTS ; ON-FIBER SILYLATION ; GAS-CHROMATOGRAPHY ; BISPHENOL-A ; WASTE-WATER ; SITU DERIVATIZATION
Citation statistics
Cited Times:110[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8804
Collection期刊论文
Corresponding AuthorFeng, YQ, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
Affiliation1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wen, Yi,Zhou, Bing-Sheng,Xu, Ying,et al. Analysis of estrogens in environmental waters using polymer monolith in-polyether ether ketone tube solid-phase microextraction combined with high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1133(1-2):21-28.
APA Wen, Yi,Zhou, Bing-Sheng,Xu, Ying,Jin, Shi-Wei,Feng, Yu-Qi,&Feng, YQ, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.(2006).Analysis of estrogens in environmental waters using polymer monolith in-polyether ether ketone tube solid-phase microextraction combined with high-performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1133(1-2),21-28.
MLA Wen, Yi,et al."Analysis of estrogens in environmental waters using polymer monolith in-polyether ether ketone tube solid-phase microextraction combined with high-performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1133.1-2(2006):21-28.
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