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学科主题: Parasitology; Veterinary Sciences
题名: Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode
作者: Li, Ai Hua; Moon, Sung-Ung; Park, Yun-Kyu; Na, Byoung-Kuk; Hwang, Myung-Gi; Oh, Chang-Mi; Cho, Shin-Hyeong; Kong, Yoon; Kim, Tong-Soo; Chung, Pyung-Rim
通讯作者: Chung, PR, Inha Univ, Coll Med, Dept Parasitol, Inchon 400712, South Korea
关键词: Taenia solium metacestode ; cysteine protease ; recombinant protein
刊名: VETERINARY PARASITOLOGY
发表日期: 2006-11-05
DOI: 10.1016/j.vetpar.2006.05.015
卷: 141, 期:3-4, 页:251-259
收录类别: SCI
文章类型: Article
部门归属: Inha Univ, Coll Med, Dept Parasitol, Inchon 400712, South Korea; Korea Ctr Dis Control & Prevent, Natl Inst Hlth, Dib Malaria & Parasit Dis, Seoul 122701, South Korea; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Gyeongsang Natl Univ, Coll Med, Dept Parasitol, Jinju 660751, South Korea; Gyeongsang Natl Univ, Coll Med, Inst Hlth Sci, Jinju 660751, South Korea; Sungkyunkwan Univ, Sch Med, Suwon 440746, South Korea; Samsung Biomed Res Inst, Dept Mol Parasitol, Suwon 440746, South Korea; Samsung Biomed Res Inst, Ctr Mol Med, Suwon 440746, South Korea
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Parasitology ; Veterinary Sciences
研究领域[WOS]: Parasitology ; Veterinary Sciences
摘要: Taenia solium metacestode, a larval pork tapeworm, is a causative agent of neurocysticercosis, one of the most common parasitic diseases in the human central nervous system. In this study, we identified a cDNA encoding for a cathepsin L-like cysteine protease from the T solium metacestode (TsCL-1) and characterized the biochemical properties of the recombinant enzyme. The cloned cDNA of 1216 bp encoded 339 amino acids with an approximate molecular weight of 37.6 kDa which containing a typical signal peptide sequence (17 amino acids), a pro-domain (106 amino acids), and a mature domain (216 amino acids). Sequence alignments of TsCL-1 showed low sequence similarity of 27.3-44.6 to cathepsin L-like cysteine proteases from other helminth parasites, but the similarity was increased to 35.9-55.0 when compared to mature domains. The bacterially expressed recombinant protein (rTsCL-1) did not show enzyme activity; however, the rTsCL-1 expressed in Pichia pastoris showed typical biochemical characteristics of cysteine proteases. It degraded human immunoglobulin G (IgG) and bovine serum albumin (BSA), but not collagen. Western blot analysis of the rTsCL-1 showed antigenicity against the sera from patients with cysticercosis, sparganosis or fascioliasis, but weak or no antigenicity against the sera from patients with paragonimiasis or clonorchiasis. (c) 2006 Published by Elsevier B.V.
英文摘要: Taenia solium metacestode, a larval pork tapeworm, is a causative agent of neurocysticercosis, one of the most common parasitic diseases in the human central nervous system. In this study, we identified a cDNA encoding for a cathepsin L-like cysteine protease from the T solium metacestode (TsCL-1) and characterized the biochemical properties of the recombinant enzyme. The cloned cDNA of 1216 bp encoded 339 amino acids with an approximate molecular weight of 37.6 kDa which containing a typical signal peptide sequence (17 amino acids), a pro-domain (106 amino acids), and a mature domain (216 amino acids). Sequence alignments of TsCL-1 showed low sequence similarity of 27.3-44.6 to cathepsin L-like cysteine proteases from other helminth parasites, but the similarity was increased to 35.9-55.0 when compared to mature domains. The bacterially expressed recombinant protein (rTsCL-1) did not show enzyme activity; however, the rTsCL-1 expressed in Pichia pastoris showed typical biochemical characteristics of cysteine proteases. It degraded human immunoglobulin G (IgG) and bovine serum albumin (BSA), but not collagen. Western blot analysis of the rTsCL-1 showed antigenicity against the sera from patients with cysticercosis, sparganosis or fascioliasis, but weak or no antigenicity against the sera from patients with paragonimiasis or clonorchiasis. (c) 2006 Published by Elsevier B.V.
关键词[WOS]: PARAGONIMUS-WESTERMANI ; PROTEINASE
语种: 英语
WOS记录号: WOS:000241720000007
ISSN号: 0304-4017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8802
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Inha Univ, Coll Med, Dept Parasitol, Inchon 400712, South Korea
2.Korea Ctr Dis Control & Prevent, Natl Inst Hlth, Dib Malaria & Parasit Dis, Seoul 122701, South Korea
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.Gyeongsang Natl Univ, Coll Med, Dept Parasitol, Jinju 660751, South Korea
5.Gyeongsang Natl Univ, Coll Med, Inst Hlth Sci, Jinju 660751, South Korea
6.Sungkyunkwan Univ, Sch Med, Suwon 440746, South Korea
7.Samsung Biomed Res Inst, Dept Mol Parasitol, Suwon 440746, South Korea
8.Samsung Biomed Res Inst, Ctr Mol Med, Suwon 440746, South Korea

Recommended Citation:
Li, Ai Hua; Moon, Sung-Ung; Park, Yun-Kyu; Na, Byoung-Kuk; Hwang, Myung-Gi; Oh, Chang-Mi; Cho, Shin-Hyeong; Kong, Yoon; Kim, Tong-Soo; Chung, Pyung-Rim.Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode,VETERINARY PARASITOLOGY,2006,141(3-4):251-259
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