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Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting
Zhu, H. P.; Ma, D. M.; Gui, J. F.; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
2006-11-01
Source PublicationCHROMOSOME RESEARCH
ISSN0967-3849
Volume14Issue:7Pages:767-776
Abstract5S ribosomal DNA (rDNA) was isolated and sequenced from the gibel carp Carassius auratus gibelio with 162 chromosomes and crucian carp Carassius auratus with 100 chromosomes, and fluorescent probes for chromosome localization were prepared to ascertain the ploidy origin and evolutionary relationship between the two species. Using fluorescence in-situ hybridization (FISH), major 5S rDNA signals were localized to the short arms of three subtelocentric chromosomes in the gibel carp and to the short arms of two subtelocentrics in the crucian carp. In addition, some minor signals were detected on other chromosomes of both species. Simultaneously, six chromosomes were microdissected from the gibel carp metaphase spreads using glass needles, and the isolated chromosomes were amplified in vitro by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). Significantly, when the DOP-PCR-generated probes prepared from each single chromosome were hybridized, three same-sized chromosomes were painted in each gibel carp metaphase, whereas only two painted chromosomes were observed in each crucian carp metaphase spread. The data indicate that gibel carp is of triploid origin in comparison with diploid crucian carp.; 5S ribosomal DNA (rDNA) was isolated and sequenced from the gibel carp Carassius auratus gibelio with 162 chromosomes and crucian carp Carassius auratus with 100 chromosomes, and fluorescent probes for chromosome localization were prepared to ascertain the ploidy origin and evolutionary relationship between the two species. Using fluorescence in-situ hybridization (FISH), major 5S rDNA signals were localized to the short arms of three subtelocentric chromosomes in the gibel carp and to the short arms of two subtelocentrics in the crucian carp. In addition, some minor signals were detected on other chromosomes of both species. Simultaneously, six chromosomes were microdissected from the gibel carp metaphase spreads using glass needles, and the isolated chromosomes were amplified in vitro by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). Significantly, when the DOP-PCR-generated probes prepared from each single chromosome were hybridized, three same-sized chromosomes were painted in each gibel carp metaphase, whereas only two painted chromosomes were observed in each crucian carp metaphase spread. The data indicate that gibel carp is of triploid origin in comparison with diploid crucian carp.
SubtypeArticle
KeywordCarassius Auratus Gibelio Chromosome Painting 5s Rdna Fluorescence In-situ Hybridization Gynogenesis Microdissection Triploidy
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Genetics & Heredity
DOI10.1007/s10577-006-1083-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000242168000005
WOS KeywordSILVER CRUCIAN CARP ; CARASSIUS-AURATUS ; FISH ; EVOLUTION ; BLOCH ; REPRODUCTION ; ORGANIZATION ; DIVERSITY ; DNA
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Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8784
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhu, H. P.,Ma, D. M.,Gui, J. F.,et al. Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting[J]. CHROMOSOME RESEARCH,2006,14(7):767-776.
APA Zhu, H. P.,Ma, D. M.,Gui, J. F.,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China.(2006).Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting.CHROMOSOME RESEARCH,14(7),767-776.
MLA Zhu, H. P.,et al."Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting".CHROMOSOME RESEARCH 14.7(2006):767-776.
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