| Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting | |
| Zhu, H. P.; Ma, D. M.; Gui, J. F.; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China | |
| 2006-11-01 | |
| Source Publication | CHROMOSOME RESEARCH
 |
| ISSN | 0967-3849 |
| Volume | 14Issue:7Pages:767-776 |
| Abstract | 5S ribosomal DNA (rDNA) was isolated and sequenced from the gibel carp Carassius auratus gibelio with 162 chromosomes and crucian carp Carassius auratus with 100 chromosomes, and fluorescent probes for chromosome localization were prepared to ascertain the ploidy origin and evolutionary relationship between the two species. Using fluorescence in-situ hybridization (FISH), major 5S rDNA signals were localized to the short arms of three subtelocentric chromosomes in the gibel carp and to the short arms of two subtelocentrics in the crucian carp. In addition, some minor signals were detected on other chromosomes of both species. Simultaneously, six chromosomes were microdissected from the gibel carp metaphase spreads using glass needles, and the isolated chromosomes were amplified in vitro by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). Significantly, when the DOP-PCR-generated probes prepared from each single chromosome were hybridized, three same-sized chromosomes were painted in each gibel carp metaphase, whereas only two painted chromosomes were observed in each crucian carp metaphase spread. The data indicate that gibel carp is of triploid origin in comparison with diploid crucian carp.; 5S ribosomal DNA (rDNA) was isolated and sequenced from the gibel carp Carassius auratus gibelio with 162 chromosomes and crucian carp Carassius auratus with 100 chromosomes, and fluorescent probes for chromosome localization were prepared to ascertain the ploidy origin and evolutionary relationship between the two species. Using fluorescence in-situ hybridization (FISH), major 5S rDNA signals were localized to the short arms of three subtelocentric chromosomes in the gibel carp and to the short arms of two subtelocentrics in the crucian carp. In addition, some minor signals were detected on other chromosomes of both species. Simultaneously, six chromosomes were microdissected from the gibel carp metaphase spreads using glass needles, and the isolated chromosomes were amplified in vitro by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). Significantly, when the DOP-PCR-generated probes prepared from each single chromosome were hybridized, three same-sized chromosomes were painted in each gibel carp metaphase, whereas only two painted chromosomes were observed in each crucian carp metaphase spread. The data indicate that gibel carp is of triploid origin in comparison with diploid crucian carp. |
| Subtype | Article |
| Keyword | Carassius Auratus Gibelio Chromosome Painting 5s Rdna Fluorescence In-situ Hybridization Gynogenesis Microdissection Triploidy |
| Department | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China |
| Subject Area | Biochemistry & Molecular Biology ; Genetics & Heredity |
| DOI | 10.1007/s10577-006-1083-0 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS Subject | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS ID | WOS:000242168000005 |
| WOS Keyword | SILVER CRUCIAN CARP ; CARASSIUS-AURATUS ; FISH ; EVOLUTION ; BLOCH ; REPRODUCTION ; ORGANIZATION ; DIVERSITY ; DNA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8784 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, H. P.,Ma, D. M.,Gui, J. F.,et al. Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting[J]. CHROMOSOME RESEARCH,2006,14(7):767-776. |
| APA | Zhu, H. P.,Ma, D. M.,Gui, J. F.,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China.(2006).Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting.CHROMOSOME RESEARCH,14(7),767-776. |
| MLA | Zhu, H. P.,et al."Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting".CHROMOSOME RESEARCH 14.7(2006):767-776. |
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