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学科主题: Biochemistry & Molecular Biology; Ecology; Evolutionary Biology
题名: Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky)
作者: Zhang, Bin; Li, Zhong-Jie; Tong, Jin-Gou; Liao, Xiao-Lin
通讯作者: Li, ZJ, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: genomic library ; microsatellites ; Siniperca chuatsi
刊名: MOLECULAR ECOLOGY NOTES
发表日期: 2006-12-01
DOI: 10.1111/j.1471-8286.2006.01495.x
卷: 6, 期:4, 页:1216-1218
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
摘要: Eighteen microsatellite markers were isolated and characterized using an enrichment protocol in the Chinese mandarin fish Siniperca chuatsi (Basilewsky), a commercially important piscivorous fish in China. Out of 48 pairs of primers designed, 18 loci exhibited polymorphism with three to six alleles (mean 4.4 alleles/locus) and average observed heterozygosity ranged from 0.633 to 0.833 (mean 0.748) in a test population from Dongting Lake of China. Except for two loci, all other 16 loci were in the Hardy-Weinberg equilibrium. These markers would be useful for such studies as population genetics, ecology and selective breeding of the Chinese mandarin fish in future.
英文摘要: Eighteen microsatellite markers were isolated and characterized using an enrichment protocol in the Chinese mandarin fish Siniperca chuatsi (Basilewsky), a commercially important piscivorous fish in China. Out of 48 pairs of primers designed, 18 loci exhibited polymorphism with three to six alleles (mean 4.4 alleles/locus) and average observed heterozygosity ranged from 0.633 to 0.833 (mean 0.748) in a test population from Dongting Lake of China. Except for two loci, all other 16 loci were in the Hardy-Weinberg equilibrium. These markers would be useful for such studies as population genetics, ecology and selective breeding of the Chinese mandarin fish in future.
关键词[WOS]: DNA
语种: 英语
WOS记录号: WOS:000242193300079
ISSN号: 1471-8278
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8774
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhang, Bin; Li, Zhong-Jie; Tong, Jin-Gou; Liao, Xiao-Lin.Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky),MOLECULAR ECOLOGY NOTES,2006,6(4):1216-1218
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