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Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening
Yuan, Li; Zhang, Xiaohua; Xiao, Nan; Dai, Lingfen; Chen, Weizhou; Jia, Chensong; Zhao, Ruohong; Hemmingsen, Sean M.; Dai, Heping; Dai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 E Lake So Rd, Wuhan 430072, Peoples R China
2006-10-17
Source PublicationDISEASES OF AQUATIC ORGANISMS
ISSN0177-5103
Volume72Issue:2Pages:93-99
AbstractWhite spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.; White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.
SubtypeArticle
KeywordWhite Spot Syndrome Virus (Wssv) Phage Display Neutralizing Antibody Single Chain Fragment Variable Regions (Scfv) Antibody
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Shantou Univ, Key Lab Marine Biol Guangdong Province, Marine Biol Lab Nanao Stn, Shantou 515900, Peoples R China; Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
Subject AreaFisheries ; Veterinary Sciences
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Veterinary Sciences
WOS SubjectFisheries ; Veterinary Sciences
WOS IDWOS:000242930700001
WOS KeywordWHITE-SPOT-SYNDROME ; SYNDROME-VIRUS WSSV ; PRAWN PENAEUS-MONODON ; MONOCLONAL-ANTIBODIES ; CAMBARUS-CLARKII ; GENOME SEQUENCE ; CELL-CULTURES ; SHRIMP ; PROTEIN ; INFECTION
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8742
Collection期刊论文
Corresponding AuthorDai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 E Lake So Rd, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Shantou Univ, Key Lab Marine Biol Guangdong Province, Marine Biol Lab Nanao Stn, Shantou 515900, Peoples R China
4.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
Recommended Citation
GB/T 7714
Yuan, Li,Zhang, Xiaohua,Xiao, Nan,et al. Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening[J]. DISEASES OF AQUATIC ORGANISMS,2006,72(2):93-99.
APA Yuan, Li.,Zhang, Xiaohua.,Xiao, Nan.,Dai, Lingfen.,Chen, Weizhou.,...&Dai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 E Lake So Rd, Wuhan 430072, Peoples R China.(2006).Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening.DISEASES OF AQUATIC ORGANISMS,72(2),93-99.
MLA Yuan, Li,et al."Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening".DISEASES OF AQUATIC ORGANISMS 72.2(2006):93-99.
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