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学科主题: Fisheries; Veterinary Sciences
题名: Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening
作者: Yuan, Li; Zhang, Xiaohua; Xiao, Nan; Dai, Lingfen; Chen, Weizhou; Jia, Chensong; Zhao, Ruohong; Hemmingsen, Sean M.; Dai, Heping
通讯作者: Dai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 E Lake So Rd, Wuhan 430072, Peoples R China
关键词: white spot syndrome virus (WSSV) ; phage display ; neutralizing antibody ; single chain fragment variable regions (scFv) antibody
刊名: DISEASES OF AQUATIC ORGANISMS
发表日期: 2006-10-17
卷: 72, 期:2, 页:93-99
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Shantou Univ, Key Lab Marine Biol Guangdong Province, Marine Biol Lab Nanao Stn, Shantou 515900, Peoples R China; Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Veterinary Sciences
摘要: White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.
英文摘要: White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.
关键词[WOS]: WHITE-SPOT-SYNDROME ; SYNDROME-VIRUS WSSV ; PRAWN PENAEUS-MONODON ; MONOCLONAL-ANTIBODIES ; CAMBARUS-CLARKII ; GENOME SEQUENCE ; CELL-CULTURES ; SHRIMP ; PROTEIN ; INFECTION
语种: 英语
WOS记录号: WOS:000242930700001
ISSN号: 0177-5103
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8742
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Shantou Univ, Key Lab Marine Biol Guangdong Province, Marine Biol Lab Nanao Stn, Shantou 515900, Peoples R China
4.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

Recommended Citation:
Yuan, Li; Zhang, Xiaohua; Xiao, Nan; Dai, Lingfen; Chen, Weizhou; Jia, Chensong; Zhao, Ruohong; Hemmingsen, Sean M.; Dai, Heping.Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening,DISEASES OF AQUATIC ORGANISMS,2006,72(2):93-99
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