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The first non-mammalian CXCR3 in a teleost fish: Gene and expression in blood cells and central nervous system in the grass carp (Ctenopharyngodon idella)
Chang, M. X.; Sun, B. J.; Nie, P.; Nie, P, Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-02-01
Source PublicationMOLECULAR IMMUNOLOGY
ISSN0161-5890
Volume44Issue:6Pages:1123-1134
AbstractA novel fish chemokine receptor gene, chemokine (C-X-C motif) receptor 3 (CXCR3)-like was isolated from the grass carp Ctenopharyngodon idella , with its full-length genomic sequence. The cDNA of grass carp CXCR3-like (gcCXCR3-like) consists of 1261 bp with a 49bp 5'-UTR and a 189 bp 3'-UTR. An open reading frame of 1023 bp encodes a 341-amino acid peptide, with seven transmembrane helices. The deduced amino acid sequence showed the same sequence identities (37.8%) with its counterparts in goat and human. The gcCXCR3-like gene consists of two exons, with one intervening intron, spaced over approximately 2 kb of genomic sequence. Phylogenetic analyses clearly demonstrated that the gcCXCR3-like resembles the CXCR3s of other vertebrates. Real-time PCR analysis showed that gcCXCR3-like was expressed in all tested organs except heart and the expression level of gcCXCR3-like was highest in brain. Flow cytometric analyses showed the positive rate of labelled leukocytes from the healthy grass carp was 17.3%, and the labelled leukocytes were divided into three types by cell sorting. Immunohistochemical localization revealed that gcCXCR3-like expressed in whole brain regions including cerebel, diencephalon, medulla oblongata, optic lobe, and rhinencephalon, and that the labelled leukocytes are actually populations of monocyte and/or phagocyte, lymphocyte and the granulocyte. It is considered that fish CXCR expression and their function may need to be investigated in both nervous and immune systems. (c) 2006 Elsevier Ltd. All rights reserved.; A novel fish chemokine receptor gene, chemokine (C-X-C motif) receptor 3 (CXCR3)-like was isolated from the grass carp Ctenopharyngodon idella , with its full-length genomic sequence. The cDNA of grass carp CXCR3-like (gcCXCR3-like) consists of 1261 bp with a 49bp 5'-UTR and a 189 bp 3'-UTR. An open reading frame of 1023 bp encodes a 341-amino acid peptide, with seven transmembrane helices. The deduced amino acid sequence showed the same sequence identities (37.8%) with its counterparts in goat and human. The gcCXCR3-like gene consists of two exons, with one intervening intron, spaced over approximately 2 kb of genomic sequence. Phylogenetic analyses clearly demonstrated that the gcCXCR3-like resembles the CXCR3s of other vertebrates. Real-time PCR analysis showed that gcCXCR3-like was expressed in all tested organs except heart and the expression level of gcCXCR3-like was highest in brain. Flow cytometric analyses showed the positive rate of labelled leukocytes from the healthy grass carp was 17.3%, and the labelled leukocytes were divided into three types by cell sorting. Immunohistochemical localization revealed that gcCXCR3-like expressed in whole brain regions including cerebel, diencephalon, medulla oblongata, optic lobe, and rhinencephalon, and that the labelled leukocytes are actually populations of monocyte and/or phagocyte, lymphocyte and the granulocyte. It is considered that fish CXCR expression and their function may need to be investigated in both nervous and immune systems. (c) 2006 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordGrass Carp Cxcr3 Expression Nervous System Immune System
DepartmentChinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Immunology
DOI10.1016/j.molimm.2006.07.280
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Immunology
WOS SubjectBiochemistry & Molecular Biology ; Immunology
WOS IDWOS:000242949700008
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; CHEMOKINE RECEPTOR CXCR3 ; MOLECULAR-CLONING ; NEURONS ; CHEMOATTRACTANT ; INTERLEUKIN-8 ; LYMPHOCYTES ; ACTIVATION ; VIRUS ; BRAIN
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8740
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chang, M. X.,Sun, B. J.,Nie, P.,et al. The first non-mammalian CXCR3 in a teleost fish: Gene and expression in blood cells and central nervous system in the grass carp (Ctenopharyngodon idella)[J]. MOLECULAR IMMUNOLOGY,2007,44(6):1123-1134.
APA Chang, M. X.,Sun, B. J.,Nie, P.,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).The first non-mammalian CXCR3 in a teleost fish: Gene and expression in blood cells and central nervous system in the grass carp (Ctenopharyngodon idella).MOLECULAR IMMUNOLOGY,44(6),1123-1134.
MLA Chang, M. X.,et al."The first non-mammalian CXCR3 in a teleost fish: Gene and expression in blood cells and central nervous system in the grass carp (Ctenopharyngodon idella)".MOLECULAR IMMUNOLOGY 44.6(2007):1123-1134.
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