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Cloning and expression of medaka dazl during ernbryogenesis and gametogenesis
Xu, Hongyan; Li, Mingyou; Gui, Jianfang; Hong, Yunhan; Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore
2007
Source PublicationGENE EXPRESSION PATTERNS
ISSN1567-133X
Volume7Issue:3Pages:332-338
AbstractThe Deleted in azoospermia family consists of RNA-binding proteins Bottle, Daz, and Daz-like (Dazl) that are expressed in the germline. Here, we report the cloning and expression of the medakafish (Oryzias latipes) dazl gene (odazl). Interestingly, although the predicted medaka Dazl protein (oDazl) contains a RRM motif and a DAZ repeat characteristic of its mammalian homologs, it lacks 80 aa at the C-terminus. By RT-PCR, RNA in situ hybridization, Western blotting and fluorescent immunohistochemistry using a rabbit anti-DazI antibody (alpha Oazl), we analyzed the expression patterns of odazl and its protein. The odazl transcript persists throughout embryogenesis and delineates with primordial germ cells. In adults, the expression of odazl RNA and its protein is restricted to germ cells of both the testis and ovary. We observed differential expression of RNA and protein at critical stages of gametogenesis. In the testis, the odazl RNA is low at premeiotic stages, abundant at meiotic stages, but absent in postmeiotic stages; whereas the oDazl protein is rich in premeiotic stages, reduced at meiotic stages, becomes barely detectable or absent in postmeiotic round spermatids or sperm, respectively. This is in sharp mature spermatozoa. In the ovary, the odazl RNA contrast to the human situation where the Dazl transcript and protein are present in and protein persist throughout oogenesis and also show differential expression at premeiotic, meiotic and postmeiotic stages. Thus, the odazl or its protein is a marker for germ cells during embryogenesis and at critical stages of gametogenesis in both sexes of medaka. (c) 2006 Elsevier B.V. All rights reserved.; The Deleted in azoospermia family consists of RNA-binding proteins Bottle, Daz, and Daz-like (Dazl) that are expressed in the germline. Here, we report the cloning and expression of the medakafish (Oryzias latipes) dazl gene (odazl). Interestingly, although the predicted medaka Dazl protein (oDazl) contains a RRM motif and a DAZ repeat characteristic of its mammalian homologs, it lacks 80 aa at the C-terminus. By RT-PCR, RNA in situ hybridization, Western blotting and fluorescent immunohistochemistry using a rabbit anti-DazI antibody (alpha Oazl), we analyzed the expression patterns of odazl and its protein. The odazl transcript persists throughout embryogenesis and delineates with primordial germ cells. In adults, the expression of odazl RNA and its protein is restricted to germ cells of both the testis and ovary. We observed differential expression of RNA and protein at critical stages of gametogenesis. In the testis, the odazl RNA is low at premeiotic stages, abundant at meiotic stages, but absent in postmeiotic stages; whereas the oDazl protein is rich in premeiotic stages, reduced at meiotic stages, becomes barely detectable or absent in postmeiotic round spermatids or sperm, respectively. This is in sharp mature spermatozoa. In the ovary, the odazl RNA contrast to the human situation where the Dazl transcript and protein are present in and protein persist throughout oogenesis and also show differential expression at premeiotic, meiotic and postmeiotic stages. Thus, the odazl or its protein is a marker for germ cells during embryogenesis and at critical stages of gametogenesis in both sexes of medaka. (c) 2006 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordDazl Germ Cells Medaka Meiosis Rna-binding Protein Spermatogenesis
DepartmentNatl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaDevelopmental Biology ; Genetics & Heredity
DOI10.1016/j.modgep.2006.08.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaDevelopmental Biology ; Genetics & Heredity
WOS SubjectDevelopmental Biology ; Genetics & Heredity
WOS IDWOS:000243540300017
WOS KeywordPRIMORDIAL GERM-CELLS ; ORYZIAS-LATIPES ; GENE ENCODES ; HOMOLOG ; RNA ; PROTEIN ; PLASM ; SPERMATOGENESIS ; DIFFERENTIATION ; XENOPUS
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Cited Times:43[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8728
Collection期刊论文
Corresponding AuthorHong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore
Affiliation1.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xu, Hongyan,Li, Mingyou,Gui, Jianfang,et al. Cloning and expression of medaka dazl during ernbryogenesis and gametogenesis[J]. GENE EXPRESSION PATTERNS,2007,7(3):332-338.
APA Xu, Hongyan,Li, Mingyou,Gui, Jianfang,Hong, Yunhan,&Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore.(2007).Cloning and expression of medaka dazl during ernbryogenesis and gametogenesis.GENE EXPRESSION PATTERNS,7(3),332-338.
MLA Xu, Hongyan,et al."Cloning and expression of medaka dazl during ernbryogenesis and gametogenesis".GENE EXPRESSION PATTERNS 7.3(2007):332-338.
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