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学科主题: Cell Biology; Endocrinology & Metabolism
题名: Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers
作者: Xia, Wei; Zhou, Li; Yao, Bo; Li, Chuang-Ju; Gui, Han-Fang
通讯作者: Gui, HF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: DMRT1 ; Sox3 ; Sox9 ; grouper ; gonad ; sex differentiation ; sex reversal ; spermatogenesis
刊名: MOLECULAR AND CELLULAR ENDOCRINOLOGY
发表日期: 2007-01-15
DOI: 10.1016/j.mce.2006.09.014
卷: 263, 期:1-2, 页:156-172
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Cell Biology ; Endocrinology & Metabolism
研究领域[WOS]: Cell Biology ; Endocrinology & Metabolism
摘要: DMRT1 has been suggested to play different roles in sex determination and gonad differentiation, because different expression patterns have been reported among different vertebrates. The groupers, since their gonads first develop as ovary and then reverse into testis, have been thought as good models to study sex differentiation and determination. In this study, we cloned the full-length cDNAs of DMRT] gene from orange-spotted grouper (Epinephelus coioides), and prepared corresponding anti-EcDMRT1] antiserum to study the relationship of DMRT] to sex reversal. One important finding is that the grouper DMRT] is not only differentially expressed in different stage gonads, but also restricted to specific stages and specific cells of spermatogenesis. Grouper DMRT1 protein exists only in spermatogonia, primary spermatocytes and secondary spermatocytes, but not in the supporting Sertoli cells. Moreover, we confirmed that EcSox3 is expressed not only in oogonia and different stage oocytes, but also in Sertoli cells and spermatogonia, and EcSox9 is expressed only in Sertoli cells. The data suggested that grouper DMRT1 might be a more specific sex differentiation gene for spermatogenesis, and play its role at the specific stages from spermatogonia to spermatocytes. In addition, no introns were found in the grouper DMRT1, and no duplicated DMRT1, genes were detected. The finding implicates that the intronless DMRT1 that is able to undergo rapid transcriptional turnover might be a significant gene for stimulating spermatogenesis in the protogynous hermaphroditic gonad. (c) 2006 Published by Elsevier Ireland Ltd.
英文摘要: DMRT1 has been suggested to play different roles in sex determination and gonad differentiation, because different expression patterns have been reported among different vertebrates. The groupers, since their gonads first develop as ovary and then reverse into testis, have been thought as good models to study sex differentiation and determination. In this study, we cloned the full-length cDNAs of DMRT] gene from orange-spotted grouper (Epinephelus coioides), and prepared corresponding anti-EcDMRT1] antiserum to study the relationship of DMRT] to sex reversal. One important finding is that the grouper DMRT] is not only differentially expressed in different stage gonads, but also restricted to specific stages and specific cells of spermatogenesis. Grouper DMRT1 protein exists only in spermatogonia, primary spermatocytes and secondary spermatocytes, but not in the supporting Sertoli cells. Moreover, we confirmed that EcSox3 is expressed not only in oogonia and different stage oocytes, but also in Sertoli cells and spermatogonia, and EcSox9 is expressed only in Sertoli cells. The data suggested that grouper DMRT1 might be a more specific sex differentiation gene for spermatogenesis, and play its role at the specific stages from spermatogonia to spermatocytes. In addition, no introns were found in the grouper DMRT1, and no duplicated DMRT1, genes were detected. The finding implicates that the intronless DMRT1 that is able to undergo rapid transcriptional turnover might be a significant gene for stimulating spermatogenesis in the protogynous hermaphroditic gonad. (c) 2006 Published by Elsevier Ireland Ltd.
关键词[WOS]: PROTANDROUS BLACK PORGY ; ANTI-MULLERIAN HORMONE ; GONADAL DIFFERENTIATION ; MOLECULAR-CLONING ; ORYZIAS-LATIPES ; ACANTHOPAGRUS-SCHLEGELI ; GENE-EXPRESSION ; CHICKEN EMBRYOS ; MEDAKA FISH ; PROTEIN
语种: 英语
WOS记录号: WOS:000243476800017
ISSN号: 0303-7207
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8726
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Xia, Wei; Zhou, Li; Yao, Bo; Li, Chuang-Ju; Gui, Jian-Fang.Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers,MOLECULAR AND CELLULAR ENDOCRINOLOGY,2007,263(1-2):156-172
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