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Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection
Dong, Cai-Wen; Zhang, Yi-Bing; Lu, Ai-Jun; Zhu, Rong; Zhang, Fu-Tie; Zhang, Qi-Ya; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2007-04-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume22Issue:4Pages:380-393
AbstractProtein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a vitally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx wash also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response. (c) 2006 Elsevier Ltd. All rights reserved.; Protein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a vitally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx wash also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response. (c) 2006 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordProtein Arginine Methyltransferase 1 Paralichthys Olivaceus Scophthalmus Maximus RhabdoVirus Virus (Smrv) Virus Infection Differential Expression Antiviral Immune Response
DepartmentChinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2006.06.010
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000244247700008
WOS KeywordI INTERFERON RECEPTOR ; N-METHYLTRANSFERASE ; PARALICHTHYS-OLIVACEUS ; SUBSTRATE-SPECIFICITY ; CELL-LINE ; JAPANESE FLOUNDER ; MX PROTEINS ; METHYLATION ; PRMT1 ; FAMILY
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8700
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Dong, Cai-Wen,Zhang, Yi-Bing,Lu, Ai-Jun,et al. Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2007,22(4):380-393.
APA Dong, Cai-Wen.,Zhang, Yi-Bing.,Lu, Ai-Jun.,Zhu, Rong.,Zhang, Fu-Tie.,...&Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2007).Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection.FISH & SHELLFISH IMMUNOLOGY,22(4),380-393.
MLA Dong, Cai-Wen,et al."Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection".FISH & SHELLFISH IMMUNOLOGY 22.4(2007):380-393.
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