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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection
作者: Dong, Cai-Wen; Zhang, Yi-Bing; Lu, Ai-Jun; Zhu, Rong; Zhang, Fu-Tie; Zhang, Qi-Ya; Gui, Jian-Fang
通讯作者: Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: protein arginine methyltransferase 1 ; Paralichthys olivaceus ; Scophthalmus maximus rhabdovirus virus (SMRV) ; virus infection ; differential expression ; antiviral immune response
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2007-04-01
DOI: 10.1016/j.fsi.2006.06.010
卷: 22, 期:4, 页:380-393
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: Protein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a vitally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx wash also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response. (c) 2006 Elsevier Ltd. All rights reserved.
英文摘要: Protein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a vitally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx wash also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response. (c) 2006 Elsevier Ltd. All rights reserved.
关键词[WOS]: I INTERFERON RECEPTOR ; N-METHYLTRANSFERASE ; PARALICHTHYS-OLIVACEUS ; SUBSTRATE-SPECIFICITY ; CELL-LINE ; JAPANESE FLOUNDER ; MX PROTEINS ; METHYLATION ; PRMT1 ; FAMILY
语种: 英语
WOS记录号: WOS:000244247700008
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8700
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Dong, Cai-Wen; Zhang, Yi-Bing; Lu, Ai-Jun; Zhu, Rong; Zhang, Fu-Tie; Zhang, Qi-Ya; Gui, Jian-Fang.Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection,FISH & SHELLFISH IMMUNOLOGY,2007,22(4):380-393
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