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The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis
Wang, Haizhen; Liu, Yongding; Gao, Xueliang; Carter, Christie L.; Liu, Zhi-Ren; Liu, ZR, Georgia State Univ, Georgia Canc Ctr, Dept Biol, Univ Plaza, Atlanta, GA 30303 USA
2007-03-08
Source PublicationCANCER LETTERS
ISSN0304-3835
Volume247Issue:1Pages:150-158
AbstractC-Phycocyanin (C-PC) from blue-green algae has been reported to have various pharmacological characteristics, including antiinflammatory and anti-tumor activities. In this study, we expressed the beta-subunit of C-PC (ref to as C-POP) in Escherichia coli. We found that the recombinant C-PC/beta has anti-cancer properties. Under the treatment of 5 mu M of the recombinant C-PC/beta, four different cancer cell lines accrued high proliferation inhibition and apoptotic induction. Substantially, a lower response occurred in non-cancer cells. We investigated the mechanism by which C-PC/beta inhibits cancer cell proliferation and induces apoptosis. We found that the C-PC/beta interacts with membrane-associated beta-tubulin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Under the treatment of the C-PC/beta, depolymerization of microtubules and actin-filaments were observed. The cells underwent apoptosis with an increase in caspase-3, and caspase-8 activities. The cell cycle was arrested at the G0/G1 phase under the treatment of C-PC/beta. In addition, the nuclear level of GAPDH decreased significantly. Decrease in the nuclear level of GAPDH prevents the cell cycle from entering into the S phase. Inhibition of cancer cell proliferation and induction of apoptosis may potentate the C-POP as a promising cancer prevention or therapy agent. (c) 2006 Elsevier Ireland Ltd. All rights reserved.; C-Phycocyanin (C-PC) from blue-green algae has been reported to have various pharmacological characteristics, including antiinflammatory and anti-tumor activities. In this study, we expressed the beta-subunit of C-PC (ref to as C-POP) in Escherichia coli. We found that the recombinant C-PC/beta has anti-cancer properties. Under the treatment of 5 mu M of the recombinant C-PC/beta, four different cancer cell lines accrued high proliferation inhibition and apoptotic induction. Substantially, a lower response occurred in non-cancer cells. We investigated the mechanism by which C-PC/beta inhibits cancer cell proliferation and induces apoptosis. We found that the C-PC/beta interacts with membrane-associated beta-tubulin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Under the treatment of the C-PC/beta, depolymerization of microtubules and actin-filaments were observed. The cells underwent apoptosis with an increase in caspase-3, and caspase-8 activities. The cell cycle was arrested at the G0/G1 phase under the treatment of C-PC/beta. In addition, the nuclear level of GAPDH decreased significantly. Decrease in the nuclear level of GAPDH prevents the cell cycle from entering into the S phase. Inhibition of cancer cell proliferation and induction of apoptosis may potentate the C-POP as a promising cancer prevention or therapy agent. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
SubtypeArticle
KeywordBeta-subunit Of C-phycocyanin Apoptosis Cell Cycle Proliferation Caspase Beta-tubulin Gapdh
DepartmentGeorgia State Univ, Georgia Canc Ctr, Dept Biol, Atlanta, GA 30303 USA; Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaOncology
DOI10.1016/j.canlet.2006.04.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaOncology
WOS SubjectOncology
WOS IDWOS:000244383900018
WOS KeywordGLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; TUBULIN ; PROTEIN ; COMPONENT
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8686
Collection期刊论文
Corresponding AuthorLiu, ZR, Georgia State Univ, Georgia Canc Ctr, Dept Biol, Univ Plaza, Atlanta, GA 30303 USA
Affiliation1.Georgia State Univ, Georgia Canc Ctr, Dept Biol, Atlanta, GA 30303 USA
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wang, Haizhen,Liu, Yongding,Gao, Xueliang,et al. The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis[J]. CANCER LETTERS,2007,247(1):150-158.
APA Wang, Haizhen,Liu, Yongding,Gao, Xueliang,Carter, Christie L.,Liu, Zhi-Ren,&Liu, ZR, Georgia State Univ, Georgia Canc Ctr, Dept Biol, Univ Plaza, Atlanta, GA 30303 USA.(2007).The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis.CANCER LETTERS,247(1),150-158.
MLA Wang, Haizhen,et al."The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis".CANCER LETTERS 247.1(2007):150-158.
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