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Cloning and characterization of cytochrome c oxidase subunit I (COXI) in Gobiocypris rarus
Pei, De-Sheng; Sun, Yong-Hua; Chen, Shang-Ping; Wang, Ya-Ping; Zhu, Zuo-Yan; Zhu, ZY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-02-01
Source PublicationDNA SEQUENCE
ISSN1042-5179
Volume18Issue:1Pages:1-8
AbstractIn study of gene expression profile in cloned embryos which derived from D. rerio embryonic nuclei and G. rarus enucleated eggs, cytochrome c oxidase subunit I (COXI) of G. rarus, exhibiting difference at expression level between cloned embryos and zebrafish embryo, was cloned. Its full cDNA length is 1654 bp and contains a 1551 bp open reading frame, encoding a 5.64 kDa protein of 516 amino acids. The alignment result shows that mitochondrion tRNA(ser) is co-transcripted with COXI, which just was the 3'-UTR of COXI. Molecular phylogenic analysis based on COXI indicates G. rarus should belong to Gobioninae, which was not in agreement with previous study according to morphological taxonomy. Comparison of DNA with cDNA shows that RNA editing phenomenon does not occur in the COXI of G. rarus.; In study of gene expression profile in cloned embryos which derived from D. rerio embryonic nuclei and G. rarus enucleated eggs, cytochrome c oxidase subunit I (COXI) of G. rarus, exhibiting difference at expression level between cloned embryos and zebrafish embryo, was cloned. Its full cDNA length is 1654 bp and contains a 1551 bp open reading frame, encoding a 5.64 kDa protein of 516 amino acids. The alignment result shows that mitochondrion tRNA(ser) is co-transcripted with COXI, which just was the 3'-UTR of COXI. Molecular phylogenic analysis based on COXI indicates G. rarus should belong to Gobioninae, which was not in agreement with previous study according to morphological taxonomy. Comparison of DNA with cDNA shows that RNA editing phenomenon does not occur in the COXI of G. rarus.
SubtypeArticle
KeywordCytochrome c Oxidase Subunit i Rna Editing Gobiocypris Rarus Danio Rerio
DepartmentChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
DOI10.1080/10425170600699752
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000244354700001
WOS KeywordMITOCHONDRIAL ; EVOLUTION ; ZEBRAFISH ; SEQUENCES ; BOOTSTRAP ; IMPACT ; FISH
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8684
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Pei, De-Sheng,Sun, Yong-Hua,Chen, Shang-Ping,et al. Cloning and characterization of cytochrome c oxidase subunit I (COXI) in Gobiocypris rarus[J]. DNA SEQUENCE,2007,18(1):1-8.
APA Pei, De-Sheng,Sun, Yong-Hua,Chen, Shang-Ping,Wang, Ya-Ping,Zhu, Zuo-Yan,&Zhu, ZY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Cloning and characterization of cytochrome c oxidase subunit I (COXI) in Gobiocypris rarus.DNA SEQUENCE,18(1),1-8.
MLA Pei, De-Sheng,et al."Cloning and characterization of cytochrome c oxidase subunit I (COXI) in Gobiocypris rarus".DNA SEQUENCE 18.1(2007):1-8.
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