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学科主题: Biotechnology & Applied Microbiology; Chemistry ; Multidisciplinary; Engineering ; Chemical
题名: Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption
作者: Chen, Wei-Dong; Wang, Yan-Dong; Hu, Han-Hua; Su, Zhi-Guo
通讯作者: Chen, WD, City Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, 1500 E Duarte Rd, Duarte, CA 91010 USA
关键词: cation resin ; hydrodynamics ; dynamic binding capacity ; expanded bed adsorption ; natural product ; separations
刊名: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
发表日期: 2007-02-01
DOI: 10.1002/jctb.1632
卷: 82, 期:2, 页:135-142
收录类别: SCI
文章类型: Article
部门归属: City Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, Duarte, CA 91010 USA; Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical
研究领域[WOS]: Biotechnology & Applied Microbiology ; Chemistry ; Engineering
摘要: Successful applications of expanded bed adsorption (EBA) technology have been widely reported in the literature for protein purification. Little has been reported on the recovery of natural products and active components of Chinese herbal preparations using EBA technology. In this study, the hydrodynamic behavior in an expanded bed of cation resin, 001 x 7 Styrene-DVB, was investigated. Ephedrine hydrochloride (EH) was used as a model natural product to test the dynamic binding capacity (DBC) in the expanded bed. EBA of EH directly from a feedstock containing powdered herbs has also been investigated. These particles are different from commercially available expanded bed adsorbents by virtue of their large size (20S to 1030 gm). When the adsorbent bed is expanded to approximately 1.3 to 1.5 times its settled bed height, the axial liquid-phase dispersion coefficient was found to be of the order 10(-5) m(2) s(-1), which falls into the range 1.0 x 10(-6) to 1.0 X 10(-5) m(2) s(-1) observed previously in protein purification. Because of the favorable column efficiency (low axial dispersion coefficient), the recovery yield and purification factor values of EH directly from a feedstock reached 86.5% and 18, respectively. The results suggest that EBA technology holds promise for the recovery of natural products and active components of Chinese herbal preparations.
英文摘要: Successful applications of expanded bed adsorption (EBA) technology have been widely reported in the literature for protein purification. Little has been reported on the recovery of natural products and active components of Chinese herbal preparations using EBA technology. In this study, the hydrodynamic behavior in an expanded bed of cation resin, 001 x 7 Styrene-DVB, was investigated. Ephedrine hydrochloride (EH) was used as a model natural product to test the dynamic binding capacity (DBC) in the expanded bed. EBA of EH directly from a feedstock containing powdered herbs has also been investigated. These particles are different from commercially available expanded bed adsorbents by virtue of their large size (20S to 1030 gm). When the adsorbent bed is expanded to approximately 1.3 to 1.5 times its settled bed height, the axial liquid-phase dispersion coefficient was found to be of the order 10(-5) m(2) s(-1), which falls into the range 1.0 x 10(-6) to 1.0 X 10(-5) m(2) s(-1) observed previously in protein purification. Because of the favorable column efficiency (low axial dispersion coefficient), the recovery yield and purification factor values of EH directly from a feedstock reached 86.5% and 18, respectively. The results suggest that EBA technology holds promise for the recovery of natural products and active components of Chinese herbal preparations.
关键词[WOS]: SUPERCRITICAL-FLUID EXTRACTION ; MONOCLONAL-ANTIBODIES ; PROTEIN ADSORPTION ; PURIFICATION ; CHROMATOGRAPHY ; PERFORMANCE ; EXPANSION ; RECOVERY ; CULTURES ; COLUMN
语种: 英语
WOS记录号: WOS:000244615300003
ISSN号: 0268-2575
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8674
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.City Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, Duarte, CA 91010 USA
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Chen, Wei-Dong; Wang, Yan-Dong; Hu, Han-Hua; Su, Zhi-Guo.Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption,JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2007,82(2):135-142
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