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Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption
Chen, Wei-Dong; Wang, Yan-Dong; Hu, Han-Hua; Su, Zhi-Guo; Chen, WD, City Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, 1500 E Duarte Rd, Duarte, CA 91010 USA
2007-02-01
Source PublicationJOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
ISSN0268-2575
Volume82Issue:2Pages:135-142
AbstractSuccessful applications of expanded bed adsorption (EBA) technology have been widely reported in the literature for protein purification. Little has been reported on the recovery of natural products and active components of Chinese herbal preparations using EBA technology. In this study, the hydrodynamic behavior in an expanded bed of cation resin, 001 x 7 Styrene-DVB, was investigated. Ephedrine hydrochloride (EH) was used as a model natural product to test the dynamic binding capacity (DBC) in the expanded bed. EBA of EH directly from a feedstock containing powdered herbs has also been investigated. These particles are different from commercially available expanded bed adsorbents by virtue of their large size (20S to 1030 gm). When the adsorbent bed is expanded to approximately 1.3 to 1.5 times its settled bed height, the axial liquid-phase dispersion coefficient was found to be of the order 10(-5) m(2) s(-1), which falls into the range 1.0 x 10(-6) to 1.0 X 10(-5) m(2) s(-1) observed previously in protein purification. Because of the favorable column efficiency (low axial dispersion coefficient), the recovery yield and purification factor values of EH directly from a feedstock reached 86.5% and 18, respectively. The results suggest that EBA technology holds promise for the recovery of natural products and active components of Chinese herbal preparations.; Successful applications of expanded bed adsorption (EBA) technology have been widely reported in the literature for protein purification. Little has been reported on the recovery of natural products and active components of Chinese herbal preparations using EBA technology. In this study, the hydrodynamic behavior in an expanded bed of cation resin, 001 x 7 Styrene-DVB, was investigated. Ephedrine hydrochloride (EH) was used as a model natural product to test the dynamic binding capacity (DBC) in the expanded bed. EBA of EH directly from a feedstock containing powdered herbs has also been investigated. These particles are different from commercially available expanded bed adsorbents by virtue of their large size (20S to 1030 gm). When the adsorbent bed is expanded to approximately 1.3 to 1.5 times its settled bed height, the axial liquid-phase dispersion coefficient was found to be of the order 10(-5) m(2) s(-1), which falls into the range 1.0 x 10(-6) to 1.0 X 10(-5) m(2) s(-1) observed previously in protein purification. Because of the favorable column efficiency (low axial dispersion coefficient), the recovery yield and purification factor values of EH directly from a feedstock reached 86.5% and 18, respectively. The results suggest that EBA technology holds promise for the recovery of natural products and active components of Chinese herbal preparations.
SubtypeArticle
KeywordCation Resin Hydrodynamics Dynamic Binding Capacity Expanded Bed Adsorption Natural Product Separations
DepartmentCity Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, Duarte, CA 91010 USA; Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaChemical ; Biotechnology & Applied Microbiology ; Multidisciplinary ; Chemistry ; Engineering
DOI10.1002/jctb.1632
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Chemistry ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:000244615300003
WOS KeywordSUPERCRITICAL-FLUID EXTRACTION ; MONOCLONAL-ANTIBODIES ; PROTEIN ADSORPTION ; PURIFICATION ; CHROMATOGRAPHY ; PERFORMANCE ; EXPANSION ; RECOVERY ; CULTURES ; COLUMN
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8674
Collection期刊论文
Corresponding AuthorChen, WD, City Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, 1500 E Duarte Rd, Duarte, CA 91010 USA
Affiliation1.City Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, Duarte, CA 91010 USA
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Wei-Dong,Wang, Yan-Dong,Hu, Han-Hua,et al. Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2007,82(2):135-142.
APA Chen, Wei-Dong,Wang, Yan-Dong,Hu, Han-Hua,Su, Zhi-Guo,&Chen, WD, City Hope Natl Med Ctr, Beckman Res Inst, Dept Gene Regulat & Drug Discovery, 1500 E Duarte Rd, Duarte, CA 91010 USA.(2007).Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,82(2),135-142.
MLA Chen, Wei-Dong,et al."Hydrodynamics in an expanded bed of large size ion-exchange resin, and natural product adsorption".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 82.2(2007):135-142.
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