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Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line
Ding, Ling; Murphy, Margaret B.; He, Yuhe; Xu, Yan; Yeung, Leo W. Y.; Wang, Jingxian; Zhou, Bingsheng; Lam, Paul K. S.; Wu, Rudolf S. S.; Giesy, John P.; Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-04-01
Source PublicationENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
ISSN0730-7268
Volume26Issue:4Pages:764-772
AbstractBrominated flame retardants (BFRs) and brominated dioxins are emerging persistent organic pollutants that are ubiquitous in the environment and can be accumulated by wildlife and humans. These chemicals can disturb endocrine function. Recent studies have demonstrated that one of the mechanisms of endocrine disruption by chemicals is modulation of steroidogenic gene expression or enzyme activities. In this study, an in vitro assay based on the H295R human adrenocortical carcinoma cell line, which possesses most key genes or enzymes involved in steroidogenesis, was used to examine the effects of five bromophenols, two polybrominated biphenyls (PBBs 77 and 169), 2,3,7,8-tetrabromodibenzo-p-dioxin, and 2,3,7,8-tetrabromodibenzofuran on the expression of 10 key steroidogenic genes. The H295R cells were exposed to various BFR concentrations for 48 h, and the expression of specific genescytochrome P450 (CYP11A, CYP11B2, CYP17, CYP19, and CYP21), 3 beta-hydroxysteroid dehydrogenase (3PHSD2), 17 beta-hydroxysteroid dehydrogenase (17 beta HSD1 and 17 beta HSD4), steroidogenic acute regulatory protein (StAR), and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR)-was quantitatively measured using real-time polymerase chain reaction. Cell viability was not affected at the doses tested. Most of the genes were either up- or down-regulated, to some extent, by BFR exposure. Among the genes tested, 3PHSD2 was the most markedly up-regulated, with a range of magnitude from 1.6- to 20-fold. The results demonstrate that bromophenol, bromobiphenyls, and bromodibenzo-p-dioxin/furan are able to modulate steroidogenic gene expression, which may lead to endocrine disruption.; Brominated flame retardants (BFRs) and brominated dioxins are emerging persistent organic pollutants that are ubiquitous in the environment and can be accumulated by wildlife and humans. These chemicals can disturb endocrine function. Recent studies have demonstrated that one of the mechanisms of endocrine disruption by chemicals is modulation of steroidogenic gene expression or enzyme activities. In this study, an in vitro assay based on the H295R human adrenocortical carcinoma cell line, which possesses most key genes or enzymes involved in steroidogenesis, was used to examine the effects of five bromophenols, two polybrominated biphenyls (PBBs 77 and 169), 2,3,7,8-tetrabromodibenzo-p-dioxin, and 2,3,7,8-tetrabromodibenzofuran on the expression of 10 key steroidogenic genes. The H295R cells were exposed to various BFR concentrations for 48 h, and the expression of specific genescytochrome P450 (CYP11A, CYP11B2, CYP17, CYP19, and CYP21), 3 beta-hydroxysteroid dehydrogenase (3PHSD2), 17 beta-hydroxysteroid dehydrogenase (17 beta HSD1 and 17 beta HSD4), steroidogenic acute regulatory protein (StAR), and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR)-was quantitatively measured using real-time polymerase chain reaction. Cell viability was not affected at the doses tested. Most of the genes were either up- or down-regulated, to some extent, by BFR exposure. Among the genes tested, 3PHSD2 was the most markedly up-regulated, with a range of magnitude from 1.6- to 20-fold. The results demonstrate that bromophenol, bromobiphenyls, and bromodibenzo-p-dioxin/furan are able to modulate steroidogenic gene expression, which may lead to endocrine disruption.
SubtypeArticle
KeywordBrominated Flame Retardants Bromodioxins Steroidogenesis H295r Gene Expression
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Hong Kong, Hong Kong, Peoples R China; Michigan State Univ, Dept Zool, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA; Michigan State Univ, Inst Environm Toxicol, E Lansing, MI 48824 USA
Subject AreaEnvironmental Sciences ; Toxicology
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000244938200023
WOS KeywordAROMATASE CYP19 ACTIVITY ; POLYBROMINATED DIPHENYL ETHERS ; DIBENZO-P-DIOXINS ; EXPRESSION ; BIOSYNTHESIS ; INHIBITION ; EXPOSURE ; CONTAMINANTS ; INDUCTION ; BIPHENYLS
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8668
Collection期刊论文
Corresponding AuthorZhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Hong Kong, Hong Kong, Peoples R China
3.Michigan State Univ, Dept Zool, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA
4.Michigan State Univ, Inst Environm Toxicol, E Lansing, MI 48824 USA
Recommended Citation
GB/T 7714
Ding, Ling,Murphy, Margaret B.,He, Yuhe,et al. Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2007,26(4):764-772.
APA Ding, Ling.,Murphy, Margaret B..,He, Yuhe.,Xu, Yan.,Yeung, Leo W. Y..,...&Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,26(4),764-772.
MLA Ding, Ling,et al."Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 26.4(2007):764-772.
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