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学科主题: Environmental Sciences; Toxicology
题名: Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line
作者: Ding, Ling; Murphy, Margaret B.; He, Yuhe; Xu, Yan; Yeung, Leo W. Y.; Wang, Jingxian; Zhou, Bingsheng; Lam, Paul K. S.; Wu, Rudolf S. S.; Giesy, John P.
通讯作者: Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: brominated flame retardants ; bromodioxins ; steroidogenesis ; H295R ; gene expression
刊名: ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
发表日期: 2007-04-01
卷: 26, 期:4, 页:764-772
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Hong Kong, Hong Kong, Peoples R China; Michigan State Univ, Dept Zool, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA; Michigan State Univ, Inst Environm Toxicol, E Lansing, MI 48824 USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences ; Toxicology
研究领域[WOS]: Environmental Sciences & Ecology ; Toxicology
摘要: Brominated flame retardants (BFRs) and brominated dioxins are emerging persistent organic pollutants that are ubiquitous in the environment and can be accumulated by wildlife and humans. These chemicals can disturb endocrine function. Recent studies have demonstrated that one of the mechanisms of endocrine disruption by chemicals is modulation of steroidogenic gene expression or enzyme activities. In this study, an in vitro assay based on the H295R human adrenocortical carcinoma cell line, which possesses most key genes or enzymes involved in steroidogenesis, was used to examine the effects of five bromophenols, two polybrominated biphenyls (PBBs 77 and 169), 2,3,7,8-tetrabromodibenzo-p-dioxin, and 2,3,7,8-tetrabromodibenzofuran on the expression of 10 key steroidogenic genes. The H295R cells were exposed to various BFR concentrations for 48 h, and the expression of specific genescytochrome P450 (CYP11A, CYP11B2, CYP17, CYP19, and CYP21), 3 beta-hydroxysteroid dehydrogenase (3PHSD2), 17 beta-hydroxysteroid dehydrogenase (17 beta HSD1 and 17 beta HSD4), steroidogenic acute regulatory protein (StAR), and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR)-was quantitatively measured using real-time polymerase chain reaction. Cell viability was not affected at the doses tested. Most of the genes were either up- or down-regulated, to some extent, by BFR exposure. Among the genes tested, 3PHSD2 was the most markedly up-regulated, with a range of magnitude from 1.6- to 20-fold. The results demonstrate that bromophenol, bromobiphenyls, and bromodibenzo-p-dioxin/furan are able to modulate steroidogenic gene expression, which may lead to endocrine disruption.
英文摘要: Brominated flame retardants (BFRs) and brominated dioxins are emerging persistent organic pollutants that are ubiquitous in the environment and can be accumulated by wildlife and humans. These chemicals can disturb endocrine function. Recent studies have demonstrated that one of the mechanisms of endocrine disruption by chemicals is modulation of steroidogenic gene expression or enzyme activities. In this study, an in vitro assay based on the H295R human adrenocortical carcinoma cell line, which possesses most key genes or enzymes involved in steroidogenesis, was used to examine the effects of five bromophenols, two polybrominated biphenyls (PBBs 77 and 169), 2,3,7,8-tetrabromodibenzo-p-dioxin, and 2,3,7,8-tetrabromodibenzofuran on the expression of 10 key steroidogenic genes. The H295R cells were exposed to various BFR concentrations for 48 h, and the expression of specific genescytochrome P450 (CYP11A, CYP11B2, CYP17, CYP19, and CYP21), 3 beta-hydroxysteroid dehydrogenase (3PHSD2), 17 beta-hydroxysteroid dehydrogenase (17 beta HSD1 and 17 beta HSD4), steroidogenic acute regulatory protein (StAR), and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR)-was quantitatively measured using real-time polymerase chain reaction. Cell viability was not affected at the doses tested. Most of the genes were either up- or down-regulated, to some extent, by BFR exposure. Among the genes tested, 3PHSD2 was the most markedly up-regulated, with a range of magnitude from 1.6- to 20-fold. The results demonstrate that bromophenol, bromobiphenyls, and bromodibenzo-p-dioxin/furan are able to modulate steroidogenic gene expression, which may lead to endocrine disruption.
关键词[WOS]: AROMATASE CYP19 ACTIVITY ; POLYBROMINATED DIPHENYL ETHERS ; DIBENZO-P-DIOXINS ; EXPRESSION ; BIOSYNTHESIS ; INHIBITION ; EXPOSURE ; CONTAMINANTS ; INDUCTION ; BIPHENYLS
语种: 英语
WOS记录号: WOS:000244938200023
ISSN号: 0730-7268
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8668
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Hong Kong, Hong Kong, Peoples R China
3.Michigan State Univ, Dept Zool, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA
4.Michigan State Univ, Inst Environm Toxicol, E Lansing, MI 48824 USA

Recommended Citation:
Ding, Ling; Murphy, Margaret B.; He, Yuhe; Xu, Yan; Yeung, Leo W. Y.; Wang, Jingxian; Zhou, Bingsheng; Lam, Paul K. S.; Wu, Rudolf S. S.; Giesy, John P..Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line,ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2007,26(4):764-772
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