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Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi
Sun, B. J.; Chang, M. X.; Song, Y.; Yao, W. J.; Nie, P.; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-03-15
Source PublicationVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN0165-2427
Volume116Issue:1-2Pages:26-36
AbstractThe genes of IRF-1 and IRF-7 have been cloned from the mandarin fish (Siniperca chuatsi). The IRF-1 gene has 4919 nucleotides (nt) and contains 10exons and 9introns, with an open reading frame (ORF) of 903 ntencoding301 aa. The IRF-7 gene has 6057 nt and also contains 10exons and 9introns, with an ORF of 1308 nt encoding 436 aa. The IRF-1 and IRF-7 genes have only one copy each in the genome. The transcription of IRF-1 and IRF-7 in different organs was analyzed by real-time PCR, and both molecules were constitutively expressed. The IRF-I and IRF-7 mRNAs were abundant in gill, spleen, kidney and pronephros. The temporal transcriptional changes for IRF-1, IRF-7 and Mx were investigated within 48 h after poly I: C stimulation in liver, gill, spleen and pronephros. An increased transcription was detected for IRF-1 and IRF-7 12 h post-stimulation, being earlier than the transcription of Mx protein; however, IRF-1 and IRF-7 transcription decreased while the Mx protein was stable at 48 h post-stimulation. (c) 2007 Published by Elsevier B.V.; The genes of IRF-1 and IRF-7 have been cloned from the mandarin fish (Siniperca chuatsi). The IRF-1 gene has 4919 nucleotides (nt) and contains 10exons and 9introns, with an open reading frame (ORF) of 903 ntencoding301 aa. The IRF-7 gene has 6057 nt and also contains 10exons and 9introns, with an ORF of 1308 nt encoding 436 aa. The IRF-1 and IRF-7 genes have only one copy each in the genome. The transcription of IRF-1 and IRF-7 in different organs was analyzed by real-time PCR, and both molecules were constitutively expressed. The IRF-I and IRF-7 mRNAs were abundant in gill, spleen, kidney and pronephros. The temporal transcriptional changes for IRF-1, IRF-7 and Mx were investigated within 48 h after poly I: C stimulation in liver, gill, spleen and pronephros. An increased transcription was detected for IRF-1 and IRF-7 12 h post-stimulation, being earlier than the transcription of Mx protein; however, IRF-1 and IRF-7 transcription decreased while the Mx protein was stable at 48 h post-stimulation. (c) 2007 Published by Elsevier B.V.
SubtypeArticle
KeywordInterferon Regulatory Factor Irf-1 Irf-7 Gene Organization Real-time Pcr Mandarin Fish Chinese Perch Siniperca Chuatsi
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Peoples R China
Subject AreaImmunology ; Veterinary Sciences
DOI10.1016/j.vetimm.2007.01.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Veterinary Sciences
WOS SubjectImmunology ; Veterinary Sciences
WOS IDWOS:000245259300003
WOS KeywordINTERFERON REGULATORY FACTOR ; PANCREATIC NECROSIS VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; DOUBLE-STRANDED-RNA ; MOLECULAR-CLONING ; EXPRESSION ; ACTIVATION ; INDUCTION ; FACTOR-7 ; MX
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8652
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Sun, B. J.,Chang, M. X.,Song, Y.,et al. Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2007,116(1-2):26-36.
APA Sun, B. J.,Chang, M. X.,Song, Y.,Yao, W. J.,Nie, P.,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,116(1-2),26-36.
MLA Sun, B. J.,et al."Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 116.1-2(2007):26-36.
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