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Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression
Wang, Na; Sun, Yong-Hua; Liu, Jing; Wang, Ya-Ping; Zhu, Zuo-Yan; Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-04-01
Source PublicationDEVELOPMENT GENES AND EVOLUTION
ISSN0949-944X
Volume217Issue:4Pages:299-305
AbstractSonic hedgehog (Shh), one of important homologous members of the hedgehog (Hh) family in vertebrates, encodes a signaling molecule that is involved in short- or long-range patterning processes during embryogenesis. In zebrafish, maternal activity of Hh was found to be contributing to the formation of primary motoneurons. However, we found that all of the known Hh members were not maternally expressed in zebrafish. In the present study, full-length cDNA of common carp (Cyprinus carpio) Shh (cShh) was gained by degenerate reverse-transcription PCR (RT-PCR) and rapid amplification of cDNA ends. Sequence comparison shows that cShh coding sequence shares 93.4% identity with zebrafish Shh coding sequence, and their corresponding protein sequences have 91.9% similarity. Comparative analysis of Shh genomic sequences and Hh protein sequences from different species revealed that the genomic structures of Hh are conserved from invertebrate to vertebrate. In contrast to zebrafish Shh, cShh transcripts were detectable from one-cell stage by RT-PCR analysis. Whole mount in situ hybridization verified the maternal expression of Shh in common carp, which is, to our knowledge, the first report of that in vertebrates, suggesting that Shh might be responsible for the maternal Hh activity in common carp.; Sonic hedgehog (Shh), one of important homologous members of the hedgehog (Hh) family in vertebrates, encodes a signaling molecule that is involved in short- or long-range patterning processes during embryogenesis. In zebrafish, maternal activity of Hh was found to be contributing to the formation of primary motoneurons. However, we found that all of the known Hh members were not maternally expressed in zebrafish. In the present study, full-length cDNA of common carp (Cyprinus carpio) Shh (cShh) was gained by degenerate reverse-transcription PCR (RT-PCR) and rapid amplification of cDNA ends. Sequence comparison shows that cShh coding sequence shares 93.4% identity with zebrafish Shh coding sequence, and their corresponding protein sequences have 91.9% similarity. Comparative analysis of Shh genomic sequences and Hh protein sequences from different species revealed that the genomic structures of Hh are conserved from invertebrate to vertebrate. In contrast to zebrafish Shh, cShh transcripts were detectable from one-cell stage by RT-PCR analysis. Whole mount in situ hybridization verified the maternal expression of Shh in common carp, which is, to our knowledge, the first report of that in vertebrates, suggesting that Shh might be responsible for the maternal Hh activity in common carp.
SubtypeArticle
KeywordCommon Carp Maternal Expression Phylogenetic Analysis Sonic Hedgehog
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaCell Biology ; Evolutionary Biology ; Developmental Biology
DOI10.1007/s00427-007-0139-2
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Evolutionary Biology ; Developmental Biology
WOS SubjectCell Biology ; Evolutionary Biology ; Developmental Biology
WOS IDWOS:000245293600005
WOS KeywordZEBRAFISH ; GENE ; EMBRYOS
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8648
Collection期刊论文
Corresponding AuthorSun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wang, Na,Sun, Yong-Hua,Liu, Jing,et al. Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression[J]. DEVELOPMENT GENES AND EVOLUTION,2007,217(4):299-305.
APA Wang, Na,Sun, Yong-Hua,Liu, Jing,Wang, Ya-Ping,Zhu, Zuo-Yan,&Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression.DEVELOPMENT GENES AND EVOLUTION,217(4),299-305.
MLA Wang, Na,et al."Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression".DEVELOPMENT GENES AND EVOLUTION 217.4(2007):299-305.
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