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学科主题: Microbiology
题名: Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc
作者: Han, D.; Hu, Z.
通讯作者: Hu, Z, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: OPTIMAL-GROWTH TEMPERATURES ; SECONDARY STRUCTURE ; COMMUNE CYANOBACTERIA ; SEQUENCE ALIGNMENT ; MESSENGER-RNA ; GENOMIC DNA ; PROKARYOTES ; SUBSTITUTION ; CONSTRAINTS ; PREDICTION
刊名: CURRENT MICROBIOLOGY
发表日期: 2007-04-01
DOI: 10.1007/s00284-006-0095-5
卷: 54, 期:4, 页:254-259
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: The ribosomal RNA molecule is an ideal model for evaluating the stability of a gene product under desiccation stress. We isolated 8 Nostoc strains that had the capacity to withstand desiccation in habitats and sequenced their 16S rRNA genes. The stabilities of 16S rRNAs secondary structures, indicated by free energy change of folding, were compared among Nostoc and other related species. The results suggested that 163 rRNA secondary structures of the desiccation-tolerant Nostoc strains were more stable than that of planktonic Nostocaceae species. The stabilizing mutations were divided into two categories: (1) those causing GC to replace other types of base pairs in stems and (2) those causing extension of stems. By mapping stabilizing mutations onto the Nostoc phylogenetic tree based on 16S rRNA gene, it was shown that most of stabilizing mutations had evolved during adaptive radiation among Nostoc spp. The evolution of 16S rRNA along the Nostoc lineage is suggested to be selectively advantageous under desiccation stress.
英文摘要: The ribosomal RNA molecule is an ideal model for evaluating the stability of a gene product under desiccation stress. We isolated 8 Nostoc strains that had the capacity to withstand desiccation in habitats and sequenced their 16S rRNA genes. The stabilities of 16S rRNAs secondary structures, indicated by free energy change of folding, were compared among Nostoc and other related species. The results suggested that 163 rRNA secondary structures of the desiccation-tolerant Nostoc strains were more stable than that of planktonic Nostocaceae species. The stabilizing mutations were divided into two categories: (1) those causing GC to replace other types of base pairs in stems and (2) those causing extension of stems. By mapping stabilizing mutations onto the Nostoc phylogenetic tree based on 16S rRNA gene, it was shown that most of stabilizing mutations had evolved during adaptive radiation among Nostoc spp. The evolution of 16S rRNA along the Nostoc lineage is suggested to be selectively advantageous under desiccation stress.
关键词[WOS]: OPTIMAL-GROWTH TEMPERATURES ; SECONDARY STRUCTURE ; COMMUNE CYANOBACTERIA ; SEQUENCE ALIGNMENT ; MESSENGER-RNA ; GENOMIC DNA ; PROKARYOTES ; SUBSTITUTION ; CONSTRAINTS ; PREDICTION
语种: 英语
WOS记录号: WOS:000245505700002
ISSN号: 0343-8651
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8644
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Han, D.; Hu, Z..Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc,CURRENT MICROBIOLOGY,2007,54(4):254-259
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