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Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc
Han, D.; Hu, Z.; Hu, Z, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-04-01
Source PublicationCURRENT MICROBIOLOGY
ISSN0343-8651
Volume54Issue:4Pages:254-259
AbstractThe ribosomal RNA molecule is an ideal model for evaluating the stability of a gene product under desiccation stress. We isolated 8 Nostoc strains that had the capacity to withstand desiccation in habitats and sequenced their 16S rRNA genes. The stabilities of 16S rRNAs secondary structures, indicated by free energy change of folding, were compared among Nostoc and other related species. The results suggested that 163 rRNA secondary structures of the desiccation-tolerant Nostoc strains were more stable than that of planktonic Nostocaceae species. The stabilizing mutations were divided into two categories: (1) those causing GC to replace other types of base pairs in stems and (2) those causing extension of stems. By mapping stabilizing mutations onto the Nostoc phylogenetic tree based on 16S rRNA gene, it was shown that most of stabilizing mutations had evolved during adaptive radiation among Nostoc spp. The evolution of 16S rRNA along the Nostoc lineage is suggested to be selectively advantageous under desiccation stress.; The ribosomal RNA molecule is an ideal model for evaluating the stability of a gene product under desiccation stress. We isolated 8 Nostoc strains that had the capacity to withstand desiccation in habitats and sequenced their 16S rRNA genes. The stabilities of 16S rRNAs secondary structures, indicated by free energy change of folding, were compared among Nostoc and other related species. The results suggested that 163 rRNA secondary structures of the desiccation-tolerant Nostoc strains were more stable than that of planktonic Nostocaceae species. The stabilizing mutations were divided into two categories: (1) those causing GC to replace other types of base pairs in stems and (2) those causing extension of stems. By mapping stabilizing mutations onto the Nostoc phylogenetic tree based on 16S rRNA gene, it was shown that most of stabilizing mutations had evolved during adaptive radiation among Nostoc spp. The evolution of 16S rRNA along the Nostoc lineage is suggested to be selectively advantageous under desiccation stress.
SubtypeArticle
KeywordOptimal-growth Temperatures Secondary Structure Commune Cyanobacteria Sequence Alignment Messenger-rna Genomic Dna Prokaryotes Substitution Constraints Prediction
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaMicrobiology
DOI10.1007/s00284-006-0095-5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000245505700002
WOS KeywordOPTIMAL-GROWTH TEMPERATURES ; SECONDARY STRUCTURE ; COMMUNE CYANOBACTERIA ; SEQUENCE ALIGNMENT ; MESSENGER-RNA ; GENOMIC DNA ; PROKARYOTES ; SUBSTITUTION ; CONSTRAINTS ; PREDICTION
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8644
Collection期刊论文
Corresponding AuthorHu, Z, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Han, D.,Hu, Z.,Hu, Z, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc[J]. CURRENT MICROBIOLOGY,2007,54(4):254-259.
APA Han, D.,Hu, Z.,&Hu, Z, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc.CURRENT MICROBIOLOGY,54(4),254-259.
MLA Han, D.,et al."Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc".CURRENT MICROBIOLOGY 54.4(2007):254-259.
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