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学科主题: Immunology; Virology
题名: Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China
作者: Huang, Youhua; Huang, Xiaohong; Zhang, Jing; Gui, Jianfang; Zhang, Qiya
通讯作者: Zhang, QY, Grad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
关键词: SARCOMA-ASSOCIATED-HERPESVIRUS ; KAPOSIS-SARCOMA ; CHEMOKINE RECEPTORS ; CYTOMEGALOVIRUS ; SEQUENCE ; ORF74 ; IRIDOVIRUS ; WITHDRAWAL ; APOPTOSIS ; SYSTEM
刊名: VIRAL IMMUNOLOGY
发表日期: 2007-03-01
DOI: 10.1089/vim.2006.0082
卷: 20, 期:1, 页:150-159
收录类别: SCI
文章类型: Article
部门归属: Grad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Virology
研究领域[WOS]: Immunology ; Virology
摘要: G protein-coupled receptors (GPCRs) constitute a large superfamily involved in various types of signal transduction pathways, and play an important role in coordinating the activation and migration of leukocytes to sites of infection and inflammation. Viral GPCRs, on the other hand, can help the virus to escape from host immune surveillance and contribute to viral pathogenesis. Lymphocystis disease virus isolated in China (LCDV-C) contains a putative homolog of cellular GPCRs, LCDV-C GPCR. In this paper, LCDV-C GPCR was cloned, and the subcellular localization and characterization of GPCR protein were investigated in fish cells. LCDV-C GPCR encoded a 325-amino acid peptide, containing a typical seven-transmembrane domain characteristic of the chemokine receptors and a conserved DRY motif that is usually essential for receptor activation. Transient transfection of GPCR-EGFP in fathead minnow (FHM) cells and epithelioma papulosum cyprini (EPC) cells indicated that LCDV-C GPCR was expressed abundantly in both the cytoplasm and nucleoplasm. Transient overexpression of GPCR in these two cells cannot induce obvious apoptosis. FHM cells stably expressing GPCR showed enhanced cell proliferation and significant anchorage-independent growth. The effects of GPCR protein on external apoptotic stimuli were examined. Few apoptotic bodies were observed in cells expressing GPCR treated with actinomycin D (ActD). Quantitative analysis of apoptotic cells indicated that a considerable decrease in the apoptotic fraction of cells expressing GPCR, compared with. the control cells, was detected after exposure to ActD and cycloheximide. These data suggest that LCDV-C GPCR may inhibit apoptosis as part of its potential mechanism in mediating cellular transformation.
英文摘要: G protein-coupled receptors (GPCRs) constitute a large superfamily involved in various types of signal transduction pathways, and play an important role in coordinating the activation and migration of leukocytes to sites of infection and inflammation. Viral GPCRs, on the other hand, can help the virus to escape from host immune surveillance and contribute to viral pathogenesis. Lymphocystis disease virus isolated in China (LCDV-C) contains a putative homolog of cellular GPCRs, LCDV-C GPCR. In this paper, LCDV-C GPCR was cloned, and the subcellular localization and characterization of GPCR protein were investigated in fish cells. LCDV-C GPCR encoded a 325-amino acid peptide, containing a typical seven-transmembrane domain characteristic of the chemokine receptors and a conserved DRY motif that is usually essential for receptor activation. Transient transfection of GPCR-EGFP in fathead minnow (FHM) cells and epithelioma papulosum cyprini (EPC) cells indicated that LCDV-C GPCR was expressed abundantly in both the cytoplasm and nucleoplasm. Transient overexpression of GPCR in these two cells cannot induce obvious apoptosis. FHM cells stably expressing GPCR showed enhanced cell proliferation and significant anchorage-independent growth. The effects of GPCR protein on external apoptotic stimuli were examined. Few apoptotic bodies were observed in cells expressing GPCR treated with actinomycin D (ActD). Quantitative analysis of apoptotic cells indicated that a considerable decrease in the apoptotic fraction of cells expressing GPCR, compared with. the control cells, was detected after exposure to ActD and cycloheximide. These data suggest that LCDV-C GPCR may inhibit apoptosis as part of its potential mechanism in mediating cellular transformation.
关键词[WOS]: SARCOMA-ASSOCIATED-HERPESVIRUS ; KAPOSIS-SARCOMA ; CHEMOKINE RECEPTORS ; CYTOMEGALOVIRUS ; SEQUENCE ; ORF74 ; IRIDOVIRUS ; WITHDRAWAL ; APOPTOSIS ; SYSTEM
语种: 英语
WOS记录号: WOS:000245710300015
ISSN号: 0882-8245
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8618
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Grad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China

Recommended Citation:
Huang, Youhua; Huang, Xiaohong; Zhang, Jing; Gui, Jianfang; Zhang, Qiya.Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China,VIRAL IMMUNOLOGY,2007,20(1):150-159
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