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Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China
Huang, Youhua; Huang, Xiaohong; Zhang, Jing; Gui, Jianfang; Zhang, Qiya; Zhang, QY, Grad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
2007-03-01
Source PublicationVIRAL IMMUNOLOGY
ISSN0882-8245
Volume20Issue:1Pages:150-159
AbstractG protein-coupled receptors (GPCRs) constitute a large superfamily involved in various types of signal transduction pathways, and play an important role in coordinating the activation and migration of leukocytes to sites of infection and inflammation. Viral GPCRs, on the other hand, can help the virus to escape from host immune surveillance and contribute to viral pathogenesis. Lymphocystis disease virus isolated in China (LCDV-C) contains a putative homolog of cellular GPCRs, LCDV-C GPCR. In this paper, LCDV-C GPCR was cloned, and the subcellular localization and characterization of GPCR protein were investigated in fish cells. LCDV-C GPCR encoded a 325-amino acid peptide, containing a typical seven-transmembrane domain characteristic of the chemokine receptors and a conserved DRY motif that is usually essential for receptor activation. Transient transfection of GPCR-EGFP in fathead minnow (FHM) cells and epithelioma papulosum cyprini (EPC) cells indicated that LCDV-C GPCR was expressed abundantly in both the cytoplasm and nucleoplasm. Transient overexpression of GPCR in these two cells cannot induce obvious apoptosis. FHM cells stably expressing GPCR showed enhanced cell proliferation and significant anchorage-independent growth. The effects of GPCR protein on external apoptotic stimuli were examined. Few apoptotic bodies were observed in cells expressing GPCR treated with actinomycin D (ActD). Quantitative analysis of apoptotic cells indicated that a considerable decrease in the apoptotic fraction of cells expressing GPCR, compared with. the control cells, was detected after exposure to ActD and cycloheximide. These data suggest that LCDV-C GPCR may inhibit apoptosis as part of its potential mechanism in mediating cellular transformation.; G protein-coupled receptors (GPCRs) constitute a large superfamily involved in various types of signal transduction pathways, and play an important role in coordinating the activation and migration of leukocytes to sites of infection and inflammation. Viral GPCRs, on the other hand, can help the virus to escape from host immune surveillance and contribute to viral pathogenesis. Lymphocystis disease virus isolated in China (LCDV-C) contains a putative homolog of cellular GPCRs, LCDV-C GPCR. In this paper, LCDV-C GPCR was cloned, and the subcellular localization and characterization of GPCR protein were investigated in fish cells. LCDV-C GPCR encoded a 325-amino acid peptide, containing a typical seven-transmembrane domain characteristic of the chemokine receptors and a conserved DRY motif that is usually essential for receptor activation. Transient transfection of GPCR-EGFP in fathead minnow (FHM) cells and epithelioma papulosum cyprini (EPC) cells indicated that LCDV-C GPCR was expressed abundantly in both the cytoplasm and nucleoplasm. Transient overexpression of GPCR in these two cells cannot induce obvious apoptosis. FHM cells stably expressing GPCR showed enhanced cell proliferation and significant anchorage-independent growth. The effects of GPCR protein on external apoptotic stimuli were examined. Few apoptotic bodies were observed in cells expressing GPCR treated with actinomycin D (ActD). Quantitative analysis of apoptotic cells indicated that a considerable decrease in the apoptotic fraction of cells expressing GPCR, compared with. the control cells, was detected after exposure to ActD and cycloheximide. These data suggest that LCDV-C GPCR may inhibit apoptosis as part of its potential mechanism in mediating cellular transformation.
SubtypeArticle
KeywordSarcoma-associated-herpesvirus Kaposis-sarcoma Chemokine Receptors Cytomegalovirus Sequence Orf74 Iridovirus Withdrawal Apoptosis System
DepartmentGrad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Subject AreaImmunology ; Virology
DOI10.1089/vim.2006.0082
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Virology
WOS SubjectImmunology ; Virology
WOS IDWOS:000245710300015
WOS KeywordSARCOMA-ASSOCIATED-HERPESVIRUS ; KAPOSIS-SARCOMA ; CHEMOKINE RECEPTORS ; CYTOMEGALOVIRUS ; SEQUENCE ; ORF74 ; IRIDOVIRUS ; WITHDRAWAL ; APOPTOSIS ; SYSTEM
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8618
Collection期刊论文
Corresponding AuthorZhang, QY, Grad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
AffiliationGrad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Huang, Youhua,Huang, Xiaohong,Zhang, Jing,et al. Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China[J]. VIRAL IMMUNOLOGY,2007,20(1):150-159.
APA Huang, Youhua,Huang, Xiaohong,Zhang, Jing,Gui, Jianfang,Zhang, Qiya,&Zhang, QY, Grad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China.(2007).Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China.VIRAL IMMUNOLOGY,20(1),150-159.
MLA Huang, Youhua,et al."Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China".VIRAL IMMUNOLOGY 20.1(2007):150-159.
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