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Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6
Liu, Jing; Sun, Yong-Hua; Wang, Na; Wang, Ya-Ping; Zhu, Zuo-Yan; Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2006-12-01
Source PublicationJOURNAL OF GENETICS
ISSN0022-1333
Volume85Issue:3Pages:171-178
AbstractSome members of hairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes. the her6 gene, a member of the HES family, is the homologue Of heS1 in mammals and chicken. In this study, the her6 gene and its full-length cDNA from the common carp (Cyprinus carpio) were isolated and characterized. The genomic sequence of common carp her6 is approximately 1.7 kb. with four exons and three introns, and the full-length cDNA of 1314 bp encodes a Putative polypeptide of 271 amino acids. To analyse the promoter sequence of common carp her6. sequences of various lengths upstream from the transcription initiation site of her6 were fused to enhanced green fluorescent. protein gene (eGFP) and introduced into zebrafish embryos by microinjection to generate transgenic embryos. Our results show that the upstream sequence of 500 bp can direct highly efficient and tissue-specific expression of eGFP in zebrafish embryos. whereas a fragment of 200 bp containing the TATA box and a partial suppressor of hairless paired site sequence (SPS) is not sufficient to drive eGFP expression in zebrafish embryos.; Some members of hairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes. the her6 gene, a member of the HES family, is the homologue Of heS1 in mammals and chicken. In this study, the her6 gene and its full-length cDNA from the common carp (Cyprinus carpio) were isolated and characterized. The genomic sequence of common carp her6 is approximately 1.7 kb. with four exons and three introns, and the full-length cDNA of 1314 bp encodes a Putative polypeptide of 271 amino acids. To analyse the promoter sequence of common carp her6. sequences of various lengths upstream from the transcription initiation site of her6 were fused to enhanced green fluorescent. protein gene (eGFP) and introduced into zebrafish embryos by microinjection to generate transgenic embryos. Our results show that the upstream sequence of 500 bp can direct highly efficient and tissue-specific expression of eGFP in zebrafish embryos. whereas a fragment of 200 bp containing the TATA box and a partial suppressor of hairless paired site sequence (SPS) is not sufficient to drive eGFP expression in zebrafish embryos.
SubtypeArticle
KeywordHer6 Common Carp Promoter Genomic Walking Expression Pattern Development
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaGenetics & Heredity
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000245879500003
WOS KeywordMEDAKA ORYZIAS-LATIPES ; GFP REPORTER GENE ; PRESOMITIC MESODERM ; VERTEBRATE SEGMENTATION ; SOMITE SEGMENTATION ; DYNAMIC EXPRESSION ; ZEBRAFISH ; NOTCH ; HAIRY ; SOMITOGENESIS
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8614
Collection期刊论文
Corresponding AuthorSun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jing,Sun, Yong-Hua,Wang, Na,et al. Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6[J]. JOURNAL OF GENETICS,2006,85(3):171-178.
APA Liu, Jing,Sun, Yong-Hua,Wang, Na,Wang, Ya-Ping,Zhu, Zuo-Yan,&Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2006).Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6.JOURNAL OF GENETICS,85(3),171-178.
MLA Liu, Jing,et al."Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6".JOURNAL OF GENETICS 85.3(2006):171-178.
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