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Fish ES cells and applications to biotechnology
Alvarez, M. Carmen; Bejar, Julia; Chen, Songlin; Hong, Yunhan; Alvarez, MC, Univ Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain
2007-03-01
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Volume9Issue:2Pages:117-127
AbstractES cells provide a promising tool for the generation of transgenic animals with site-directed mutations. When ES cells colonize germ cells in chimeras, transgenic animals with modified phenotypes are generated and used either for functional genomics studies or for improving productivity in commercial settings. Althought the ES cell approach has been limited to, mice, there is strong interest for developing the technology in fish.. We describe the step-by-step procedure for developing ES cells in fish. Key aspects include avoiding cell differentiation, specific in vitro traits of pluripotency, and, most importantly, testing for production of chimeric animals as the main evidence of pluripotency. The entire process focuses on two model species, zebrafish and medaka, in which most work has been done. The achievements attained in these species, as well as their applicability to other commercial fish, are discussed. Because of the difficulties relating to germ line competence, mostly of long-term fish ES cells, alternative cell-based approaches such as primordial germ cells and nuclear transfer need to be considered. Although progress to date has been slow, there are promising achievements in homologous recombination and alternative avenues yet to be explored that can bring ES technology in fish to fruition.; ES cells provide a promising tool for the generation of transgenic animals with site-directed mutations. When ES cells colonize germ cells in chimeras, transgenic animals with modified phenotypes are generated and used either for functional genomics studies or for improving productivity in commercial settings. Althought the ES cell approach has been limited to, mice, there is strong interest for developing the technology in fish.. We describe the step-by-step procedure for developing ES cells in fish. Key aspects include avoiding cell differentiation, specific in vitro traits of pluripotency, and, most importantly, testing for production of chimeric animals as the main evidence of pluripotency. The entire process focuses on two model species, zebrafish and medaka, in which most work has been done. The achievements attained in these species, as well as their applicability to other commercial fish, are discussed. Because of the difficulties relating to germ line competence, mostly of long-term fish ES cells, alternative cell-based approaches such as primordial germ cells and nuclear transfer need to be considered. Although progress to date has been slow, there are promising achievements in homologous recombination and alternative avenues yet to be explored that can bring ES technology in fish to fruition.
SubtypeReview
KeywordChimeras Es Cells Fish Transgenics
DepartmentUniv Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain; Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
Subject AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI10.1007/s10126-006-6034-4
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000245949800001
WOS KeywordEMBRYONIC STEM-CELLS ; MEDAKA ORYZIAS-LATIPES ; GERM-LINE CHIMERAS ; GREEN FLUORESCENT PROTEIN ; EARLY ZEBRAFISH EMBRYOS ; VASA-LIKE GENE ; IN-VITRO ; TELOMERASE ACTIVITY ; HOMOLOGOUS RECOMBINATION ; HUMAN BLASTOCYSTS
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8612
Collection期刊论文
Corresponding AuthorAlvarez, MC, Univ Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain
Affiliation1.Univ Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain
2.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
Recommended Citation
GB/T 7714
Alvarez, M. Carmen,Bejar, Julia,Chen, Songlin,et al. Fish ES cells and applications to biotechnology[J]. MARINE BIOTECHNOLOGY,2007,9(2):117-127.
APA Alvarez, M. Carmen,Bejar, Julia,Chen, Songlin,Hong, Yunhan,&Alvarez, MC, Univ Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain.(2007).Fish ES cells and applications to biotechnology.MARINE BIOTECHNOLOGY,9(2),117-127.
MLA Alvarez, M. Carmen,et al."Fish ES cells and applications to biotechnology".MARINE BIOTECHNOLOGY 9.2(2007):117-127.
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