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Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio
Zhu, Hua-Ping; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
2007-05-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume265Issue:1-4Pages:109-117
AbstractThe unusual allotetraploid form with unequal contribution of chromosome sets was discovered from the gynogenetic offspring of Carassius auratus gibelio stimulated by red common carp sperm. In this study, genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) with 45S rDNA probe are used. The GISH results lead to the identification of species-specific chromosomes, which permits to demonstrate the origin and genome organization in the allotetraploid form. Moreover, chromosome localization of 45S rDNA and co-localizations of 45S rDNA and Cyprinus carpio genomic DNA further confirm that one extra 45S rDNA positive chromosome in the allotetraploid form originates from the paternal haploid genome of C carpio, and other 5 45S rDNA-containing chromosomes are from the maternal genome of Carassius auratus gibelio. And, the correlation between 45 rDNA and the nucleolar organizer regions (NORs) is confirmed by silver nitrate staining. The data provide direct experiment evidence that the allotetraploid actually contains three chromosome sets of Carassius auratus gibelio and one chromosome set of C carpio, and will be a useful genetic material for both basic research and breeding practice. (c) 2006 Elsevier B.V. All rights reserved.; The unusual allotetraploid form with unequal contribution of chromosome sets was discovered from the gynogenetic offspring of Carassius auratus gibelio stimulated by red common carp sperm. In this study, genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) with 45S rDNA probe are used. The GISH results lead to the identification of species-specific chromosomes, which permits to demonstrate the origin and genome organization in the allotetraploid form. Moreover, chromosome localization of 45S rDNA and co-localizations of 45S rDNA and Cyprinus carpio genomic DNA further confirm that one extra 45S rDNA positive chromosome in the allotetraploid form originates from the paternal haploid genome of C carpio, and other 5 45S rDNA-containing chromosomes are from the maternal genome of Carassius auratus gibelio. And, the correlation between 45 rDNA and the nucleolar organizer regions (NORs) is confirmed by silver nitrate staining. The data provide direct experiment evidence that the allotetraploid actually contains three chromosome sets of Carassius auratus gibelio and one chromosome set of C carpio, and will be a useful genetic material for both basic research and breeding practice. (c) 2006 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordAllotetraploid Carassius Auratus Gibelio Genomic In Situ Hybridization Fluorescence In Situ Hybridization 45s Rdna Genome Organization Nucleolar Organizer Regions (Nors)
DepartmentChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1016/j.aquaculture.2006.10.026
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000246128800013
WOS KeywordIN-SITU HYBRIDIZATION ; SILVER CRUCIAN CARP ; X COMMON CARP ; GYNOGENETIC FISH ; PROGENY ; HYBRIDS ; BLOCH ; CHROMOSOMES ; CLONES ; LOCALIZATION
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Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8606
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Hua-Ping,Gui, Jian-Fang,Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China. Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio[J]. AQUACULTURE,2007,265(1-4):109-117.
APA Zhu, Hua-Ping,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China.(2007).Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio.AQUACULTURE,265(1-4),109-117.
MLA Zhu, Hua-Ping,et al."Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio".AQUACULTURE 265.1-4(2007):109-117.
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