| Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio | |
| Zhu, Hua-Ping; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China | |
| 2007-05-01 | |
| Source Publication | AQUACULTURE
 |
| ISSN | 0044-8486 |
| Volume | 265Issue:1-4Pages:109-117 |
| Abstract | The unusual allotetraploid form with unequal contribution of chromosome sets was discovered from the gynogenetic offspring of Carassius auratus gibelio stimulated by red common carp sperm. In this study, genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) with 45S rDNA probe are used. The GISH results lead to the identification of species-specific chromosomes, which permits to demonstrate the origin and genome organization in the allotetraploid form. Moreover, chromosome localization of 45S rDNA and co-localizations of 45S rDNA and Cyprinus carpio genomic DNA further confirm that one extra 45S rDNA positive chromosome in the allotetraploid form originates from the paternal haploid genome of C carpio, and other 5 45S rDNA-containing chromosomes are from the maternal genome of Carassius auratus gibelio. And, the correlation between 45 rDNA and the nucleolar organizer regions (NORs) is confirmed by silver nitrate staining. The data provide direct experiment evidence that the allotetraploid actually contains three chromosome sets of Carassius auratus gibelio and one chromosome set of C carpio, and will be a useful genetic material for both basic research and breeding practice. (c) 2006 Elsevier B.V. All rights reserved.; The unusual allotetraploid form with unequal contribution of chromosome sets was discovered from the gynogenetic offspring of Carassius auratus gibelio stimulated by red common carp sperm. In this study, genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) with 45S rDNA probe are used. The GISH results lead to the identification of species-specific chromosomes, which permits to demonstrate the origin and genome organization in the allotetraploid form. Moreover, chromosome localization of 45S rDNA and co-localizations of 45S rDNA and Cyprinus carpio genomic DNA further confirm that one extra 45S rDNA positive chromosome in the allotetraploid form originates from the paternal haploid genome of C carpio, and other 5 45S rDNA-containing chromosomes are from the maternal genome of Carassius auratus gibelio. And, the correlation between 45 rDNA and the nucleolar organizer regions (NORs) is confirmed by silver nitrate staining. The data provide direct experiment evidence that the allotetraploid actually contains three chromosome sets of Carassius auratus gibelio and one chromosome set of C carpio, and will be a useful genetic material for both basic research and breeding practice. (c) 2006 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Allotetraploid Carassius Auratus Gibelio Genomic In Situ Hybridization Fluorescence In Situ Hybridization 45s Rdna Genome Organization Nucleolar Organizer Regions (Nors) |
| Department | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| Subject Area | Fisheries ; Marine & Freshwater Biology |
| DOI | 10.1016/j.aquaculture.2006.10.026 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000246128800013 |
| WOS Keyword | IN-SITU HYBRIDIZATION ; SILVER CRUCIAN CARP ; X COMMON CARP ; GYNOGENETIC FISH ; PROGENY ; HYBRIDS ; BLOCH ; CHROMOSOMES ; CLONES ; LOCALIZATION |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8606 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, Hua-Ping,Gui, Jian-Fang,Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China. Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio[J]. AQUACULTURE,2007,265(1-4):109-117. |
| APA | Zhu, Hua-Ping,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China.(2007).Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio.AQUACULTURE,265(1-4),109-117. |
| MLA | Zhu, Hua-Ping,et al."Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio".AQUACULTURE 265.1-4(2007):109-117. |
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