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学科主题: Zoology
题名: Construction and characterization of a Lipotes vexillifer genomic DNA BAC library
作者: Du, Bo; Zhang, Xian-feng; Fang, Sheng-guo; Wang, Ding
通讯作者: Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: Lipotes vexillifer ; genomic DNA library ; alpha lactalbumin gene ; gene clone ; endangered species
刊名: ZOOLOGICAL SCIENCE
发表日期: 2007-04-01
DOI: 10.2108/zsj.24.401
卷: 24, 期:4, 页:401-407
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Zhejiang Univ, Key Lab Conservat Genet & Reprod Biol Endangered, Educ Minist, Hangzhou 310029, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Zoology
研究领域[WOS]: Zoology
摘要: We constructed a genomic DNA library for Lipotes vexillifer (L. vexillifer), the Baiji or Yangtze River dolphin, one of the most endangered mammals in the world. The library consists of 149,000 BAC clones, with an average insert size of 83 kb, representing approximately 3.4 haploid genome equivalents. PCR amplification of four known L. vexillifer genes yielded two to four positive clones each. To demonstrate the utility of this library, we isolated and sequenced the L. vexillifer alpha lactalbumin gene, which is a gene specific to mammals and one which has been widely used as molecular tool in phylogenetic analysis. We also end-sequenced 20 randomly selected clones, resulting in the identification of at least five new L. vexilliter genes, five SSR loci, and one SINE locus. These results suggest that this library is a valuable resource for candidate gene cloning, physical mapping, and genome sequencing of this important and threatened species.
英文摘要: We constructed a genomic DNA library for Lipotes vexillifer (L. vexillifer), the Baiji or Yangtze River dolphin, one of the most endangered mammals in the world. The library consists of 149,000 BAC clones, with an average insert size of 83 kb, representing approximately 3.4 haploid genome equivalents. PCR amplification of four known L. vexillifer genes yielded two to four positive clones each. To demonstrate the utility of this library, we isolated and sequenced the L. vexillifer alpha lactalbumin gene, which is a gene specific to mammals and one which has been widely used as molecular tool in phylogenetic analysis. We also end-sequenced 20 randomly selected clones, resulting in the identification of at least five new L. vexilliter genes, five SSR loci, and one SINE locus. These results suggest that this library is a valuable resource for candidate gene cloning, physical mapping, and genome sequencing of this important and threatened species.
关键词[WOS]: ARTIFICIAL CHROMOSOME LIBRARY ; PLANT ARABIDOPSIS-THALIANA ; RESISTANCE GENE ; CETACEAN LINEAGES ; RIVER DOLPHINS ; SEQUENCE ; IDENTIFICATION ; WHALES ; MITOCHONDRIAL ; PROJECT
语种: 英语
WOS记录号: WOS:000246955600011
ISSN号: 0289-0003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8556
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Zhejiang Univ, Key Lab Conservat Genet & Reprod Biol Endangered, Educ Minist, Hangzhou 310029, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Du, Bo; Zhang, Xian-feng; Fang, Sheng-guo; Wang, Ding.Construction and characterization of a Lipotes vexillifer genomic DNA BAC library,ZOOLOGICAL SCIENCE,2007,24(4):401-407
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