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Construction and characterization of a Lipotes vexillifer genomic DNA BAC library
Du, Bo; Zhang, Xian-feng; Fang, Sheng-guo; Wang, Ding; Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2007-04-01
Source PublicationZOOLOGICAL SCIENCE
ISSN0289-0003
Volume24Issue:4Pages:401-407
AbstractWe constructed a genomic DNA library for Lipotes vexillifer (L. vexillifer), the Baiji or Yangtze River dolphin, one of the most endangered mammals in the world. The library consists of 149,000 BAC clones, with an average insert size of 83 kb, representing approximately 3.4 haploid genome equivalents. PCR amplification of four known L. vexillifer genes yielded two to four positive clones each. To demonstrate the utility of this library, we isolated and sequenced the L. vexillifer alpha lactalbumin gene, which is a gene specific to mammals and one which has been widely used as molecular tool in phylogenetic analysis. We also end-sequenced 20 randomly selected clones, resulting in the identification of at least five new L. vexilliter genes, five SSR loci, and one SINE locus. These results suggest that this library is a valuable resource for candidate gene cloning, physical mapping, and genome sequencing of this important and threatened species.; We constructed a genomic DNA library for Lipotes vexillifer (L. vexillifer), the Baiji or Yangtze River dolphin, one of the most endangered mammals in the world. The library consists of 149,000 BAC clones, with an average insert size of 83 kb, representing approximately 3.4 haploid genome equivalents. PCR amplification of four known L. vexillifer genes yielded two to four positive clones each. To demonstrate the utility of this library, we isolated and sequenced the L. vexillifer alpha lactalbumin gene, which is a gene specific to mammals and one which has been widely used as molecular tool in phylogenetic analysis. We also end-sequenced 20 randomly selected clones, resulting in the identification of at least five new L. vexilliter genes, five SSR loci, and one SINE locus. These results suggest that this library is a valuable resource for candidate gene cloning, physical mapping, and genome sequencing of this important and threatened species.
SubtypeArticle
KeywordLipotes Vexillifer Genomic Dna Library Alpha Lactalbumin Gene Gene Clone Endangered Species
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Zhejiang Univ, Key Lab Conservat Genet & Reprod Biol Endangered, Educ Minist, Hangzhou 310029, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaZoology
DOI10.2108/zsj.24.401
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaZoology
WOS SubjectZoology
WOS IDWOS:000246955600011
WOS KeywordARTIFICIAL CHROMOSOME LIBRARY ; PLANT ARABIDOPSIS-THALIANA ; RESISTANCE GENE ; CETACEAN LINEAGES ; RIVER DOLPHINS ; SEQUENCE ; IDENTIFICATION ; WHALES ; MITOCHONDRIAL ; PROJECT
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8556
Collection期刊论文
Corresponding AuthorWang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Zhejiang Univ, Key Lab Conservat Genet & Reprod Biol Endangered, Educ Minist, Hangzhou 310029, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Du, Bo,Zhang, Xian-feng,Fang, Sheng-guo,et al. Construction and characterization of a Lipotes vexillifer genomic DNA BAC library[J]. ZOOLOGICAL SCIENCE,2007,24(4):401-407.
APA Du, Bo,Zhang, Xian-feng,Fang, Sheng-guo,Wang, Ding,&Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2007).Construction and characterization of a Lipotes vexillifer genomic DNA BAC library.ZOOLOGICAL SCIENCE,24(4),401-407.
MLA Du, Bo,et al."Construction and characterization of a Lipotes vexillifer genomic DNA BAC library".ZOOLOGICAL SCIENCE 24.4(2007):401-407.
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