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Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara)
Zhou, Guang-Zhou; Li, Zheng-Qiu; Yuan, Xiu-Ping; Zhang, Qi-Ya; Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-05-01
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Volume9Issue:3Pages:370-376
AbstractA marine fish cell line from the snout of red spotted grouper Epinephelus akaara, a protogynous hermaphrodite, was established, characterized, and subcultured with more than 60 passages. The grouper snout cell line (GSC) cells multiplied well in Dulbecco's modified Eagle's medium (DMEM) medium supplemented with 10% fetal bovine serum. The optimal growth temperature was 25 degrees C, and morphologically the cells were fibroblastic. Chromosome analysis revealed that the GSC cell line has a normal diploid karyotype with 2n = 8st + 40t. A virus titration study indicated that the cells were susceptible to turbot Scophthalmus Maximus rhabdovirus (SMRV) (10(8.5) TCID50 ml(-1)), while the viral titer of frog Rana grylio virus 9807 (RGV(9807)) reached 10(3.5) TCID50 ml-1. The infection was confirmed by cytopathic effect (CPE), immunofluorescence, and electron microscopy experiments, which detected the viral particles in the cytoplasm of virus-infected cells, respectively. Further, significant fluorescent signals were observed when the GSC cells were transfected with pEGFP vector DNA, indicating their potential utility for transgenic and genetic manipulation studies.; A marine fish cell line from the snout of red spotted grouper Epinephelus akaara, a protogynous hermaphrodite, was established, characterized, and subcultured with more than 60 passages. The grouper snout cell line (GSC) cells multiplied well in Dulbecco's modified Eagle's medium (DMEM) medium supplemented with 10% fetal bovine serum. The optimal growth temperature was 25 degrees C, and morphologically the cells were fibroblastic. Chromosome analysis revealed that the GSC cell line has a normal diploid karyotype with 2n = 8st + 40t. A virus titration study indicated that the cells were susceptible to turbot Scophthalmus Maximus rhabdovirus (SMRV) (10(8.5) TCID50 ml(-1)), while the viral titer of frog Rana grylio virus 9807 (RGV(9807)) reached 10(3.5) TCID50 ml-1. The infection was confirmed by cytopathic effect (CPE), immunofluorescence, and electron microscopy experiments, which detected the viral particles in the cytoplasm of virus-infected cells, respectively. Further, significant fluorescent signals were observed when the GSC cells were transfected with pEGFP vector DNA, indicating their potential utility for transgenic and genetic manipulation studies.
SubtypeArticle
KeywordFish Cell Line Iridovirus Red Spotted Grouper Epinephelus Akaara Rhabdovirus Transfection
DepartmentChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI10.1007/s10126-006-7165-3
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000247207600008
WOS KeywordRANA-GRYLIO VIRUS ; MOLECULAR-CLONING ; FISH ; COIOIDES ; RHABDOVIRUS ; IRIDOVIRUS ; EXPRESSION ; MANAGEMENT ; NODAVIRUS ; CHIMERAS
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8550
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Guang-Zhou,Li, Zheng-Qiu,Yuan, Xiu-Ping,et al. Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara)[J]. MARINE BIOTECHNOLOGY,2007,9(3):370-376.
APA Zhou, Guang-Zhou,Li, Zheng-Qiu,Yuan, Xiu-Ping,Zhang, Qi-Ya,&Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara).MARINE BIOTECHNOLOGY,9(3),370-376.
MLA Zhou, Guang-Zhou,et al."Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara)".MARINE BIOTECHNOLOGY 9.3(2007):370-376.
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