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Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus)
Cheng, L.; Liao, X.; Yu, X.; Tong, J.; Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007
Source PublicationANIMAL BIOTECHNOLOGY
ISSN1049-5398
Volume18Issue:3Pages:143-152
AbstractType I markers are useful for comparative mapping and other genetic analyses, but relatively difficult to develop. In the present study a microsatellite (SSR)-enriched cDNA library was constructed for the first time using the fast isolation by AFLP of sequences containing repeats (FIASCO) method in a small fish, Chinese rare minnow (Gobiocypris rarus). A total of 97.4% of the expressed sequence tags (ESTs) contained targeted CA-repeats, in which 29 unique EST-SSRs were identified. Ten out of the 28 loci for which primer pairs were designed were polymorphic with alleles ranging from three to seven (mean 4.50). Some of these EST-SSRs can be amplified in other species. These results proved that cDNA-FIASCO is an efficient way to isolate novel EST-SSRs in a fish.; Type I markers are useful for comparative mapping and other genetic analyses, but relatively difficult to develop. In the present study a microsatellite (SSR)-enriched cDNA library was constructed for the first time using the fast isolation by AFLP of sequences containing repeats (FIASCO) method in a small fish, Chinese rare minnow (Gobiocypris rarus). A total of 97.4% of the expressed sequence tags (ESTs) contained targeted CA-repeats, in which 29 unique EST-SSRs were identified. Ten out of the 28 loci for which primer pairs were designed were polymorphic with alleles ranging from three to seven (mean 4.50). Some of these EST-SSRs can be amplified in other species. These results proved that cDNA-FIASCO is an efficient way to isolate novel EST-SSRs in a fish.
SubtypeArticle
KeywordCdna Library Expressed Sequence Tag Fiasco Fish Microsatellite
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China
Subject AreaAgriculture ; Dairy & Animal Science ; Biotechnology & Applied Microbiology
DOI10.1080/10495390601054980
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology
WOS SubjectAgriculture, Dairy & Animal Science ; Biotechnology & Applied Microbiology
WOS IDWOS:000247762500001
WOS KeywordEXPRESSED SEQUENCE TAGS ; MICROSATELLITE MARKERS ; DNA ; TRANSFERABILITY ; CATFISH ; CDNA
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8526
Collection期刊论文
Corresponding AuthorTong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Cheng, L.,Liao, X.,Yu, X.,et al. Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus)[J]. ANIMAL BIOTECHNOLOGY,2007,18(3):143-152.
APA Cheng, L.,Liao, X.,Yu, X.,Tong, J.,&Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus).ANIMAL BIOTECHNOLOGY,18(3),143-152.
MLA Cheng, L.,et al."Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus)".ANIMAL BIOTECHNOLOGY 18.3(2007):143-152.
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