| Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus) | |
| Cheng, L.; Liao, X.; Yu, X.; Tong, J.; Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2007 | |
| Source Publication | ANIMAL BIOTECHNOLOGY
 |
| ISSN | 1049-5398 |
| Volume | 18Issue:3Pages:143-152 |
| Abstract | Type I markers are useful for comparative mapping and other genetic analyses, but relatively difficult to develop. In the present study a microsatellite (SSR)-enriched cDNA library was constructed for the first time using the fast isolation by AFLP of sequences containing repeats (FIASCO) method in a small fish, Chinese rare minnow (Gobiocypris rarus). A total of 97.4% of the expressed sequence tags (ESTs) contained targeted CA-repeats, in which 29 unique EST-SSRs were identified. Ten out of the 28 loci for which primer pairs were designed were polymorphic with alleles ranging from three to seven (mean 4.50). Some of these EST-SSRs can be amplified in other species. These results proved that cDNA-FIASCO is an efficient way to isolate novel EST-SSRs in a fish.; Type I markers are useful for comparative mapping and other genetic analyses, but relatively difficult to develop. In the present study a microsatellite (SSR)-enriched cDNA library was constructed for the first time using the fast isolation by AFLP of sequences containing repeats (FIASCO) method in a small fish, Chinese rare minnow (Gobiocypris rarus). A total of 97.4% of the expressed sequence tags (ESTs) contained targeted CA-repeats, in which 29 unique EST-SSRs were identified. Ten out of the 28 loci for which primer pairs were designed were polymorphic with alleles ranging from three to seven (mean 4.50). Some of these EST-SSRs can be amplified in other species. These results proved that cDNA-FIASCO is an efficient way to isolate novel EST-SSRs in a fish. |
| Subtype | Article |
| Keyword | Cdna Library Expressed Sequence Tag Fiasco Fish Microsatellite |
| Department | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China |
| Subject Area | Agriculture ; Dairy & Animal Science ; Biotechnology & Applied Microbiology |
| DOI | 10.1080/10495390601054980 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Agriculture ; Biotechnology & Applied Microbiology |
| WOS Subject | Agriculture, Dairy & Animal Science ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000247762500001 |
| WOS Keyword | EXPRESSED SEQUENCE TAGS ; MICROSATELLITE MARKERS ; DNA ; TRANSFERABILITY ; CATFISH ; CDNA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8526 |
| Collection | 期刊论文 |
| Corresponding Author | Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Cheng, L.,Liao, X.,Yu, X.,et al. Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus)[J]. ANIMAL BIOTECHNOLOGY,2007,18(3):143-152. |
| APA | Cheng, L.,Liao, X.,Yu, X.,Tong, J.,&Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus).ANIMAL BIOTECHNOLOGY,18(3),143-152. |
| MLA | Cheng, L.,et al."Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus)".ANIMAL BIOTECHNOLOGY 18.3(2007):143-152. |
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