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学科主题: Agriculture ; Dairy & Animal Science; Biotechnology & Applied Microbiology
题名: Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus)
作者: Cheng, L.; Liao, X.; Yu, X.; Tong, J.
通讯作者: Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: cDNA library ; expressed sequence tag ; FIASCO ; fish ; microsatellite
刊名: ANIMAL BIOTECHNOLOGY
发表日期: 2007
DOI: 10.1080/10495390601054980
卷: 18, 期:3, 页:143-152
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Agriculture, Dairy & Animal Science ; Biotechnology & Applied Microbiology
研究领域[WOS]: Agriculture ; Biotechnology & Applied Microbiology
摘要: Type I markers are useful for comparative mapping and other genetic analyses, but relatively difficult to develop. In the present study a microsatellite (SSR)-enriched cDNA library was constructed for the first time using the fast isolation by AFLP of sequences containing repeats (FIASCO) method in a small fish, Chinese rare minnow (Gobiocypris rarus). A total of 97.4% of the expressed sequence tags (ESTs) contained targeted CA-repeats, in which 29 unique EST-SSRs were identified. Ten out of the 28 loci for which primer pairs were designed were polymorphic with alleles ranging from three to seven (mean 4.50). Some of these EST-SSRs can be amplified in other species. These results proved that cDNA-FIASCO is an efficient way to isolate novel EST-SSRs in a fish.
英文摘要: Type I markers are useful for comparative mapping and other genetic analyses, but relatively difficult to develop. In the present study a microsatellite (SSR)-enriched cDNA library was constructed for the first time using the fast isolation by AFLP of sequences containing repeats (FIASCO) method in a small fish, Chinese rare minnow (Gobiocypris rarus). A total of 97.4% of the expressed sequence tags (ESTs) contained targeted CA-repeats, in which 29 unique EST-SSRs were identified. Ten out of the 28 loci for which primer pairs were designed were polymorphic with alleles ranging from three to seven (mean 4.50). Some of these EST-SSRs can be amplified in other species. These results proved that cDNA-FIASCO is an efficient way to isolate novel EST-SSRs in a fish.
关键词[WOS]: EXPRESSED SEQUENCE TAGS ; MICROSATELLITE MARKERS ; DNA ; TRANSFERABILITY ; CATFISH ; CDNA
语种: 英语
WOS记录号: WOS:000247762500001
ISSN号: 1049-5398
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8526
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China

Recommended Citation:
Cheng, L.; Liao, X.; Yu, X.; Tong, J..Development of EST-SSRs by an efficient FIASCO-based strategy: A case study in rare minnow (Gobiocyrpis rarus),ANIMAL BIOTECHNOLOGY,2007,18(3):143-152
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