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Isolation and characterization of polymorphic microsatellites in a sex-reversal fish, rice field eel (Monopterus albus)
Li, W. T.; Liao, X. L.; Yu, X. M.; Cheng, L.; Tong, J.; Tong, J, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2007-07-01
Source PublicationMOLECULAR ECOLOGY NOTES
ISSN1471-8278
Volume7Issue:4Pages:705-707
AbstractThe rice field eel (Monopterus albus) is a fish of economic importance in China and some Asian countries. From a (GT)(n)-enriched genomic library, 30 microsatellites were developed by employing the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Thirteen loci exhibited polymorphism with two to 13 alleles (mean 7.9 alleles/locus) in a test population and observed heterozygosity ranged from 0.3125 to 0.9688 (mean 0.7140). These loci should provide sufficient level of genetic variation to study the fine-scale population structure and reproductive ecology of the species.; The rice field eel (Monopterus albus) is a fish of economic importance in China and some Asian countries. From a (GT)(n)-enriched genomic library, 30 microsatellites were developed by employing the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Thirteen loci exhibited polymorphism with two to 13 alleles (mean 7.9 alleles/locus) in a test population and observed heterozygosity ranged from 0.3125 to 0.9688 (mean 0.7140). These loci should provide sufficient level of genetic variation to study the fine-scale population structure and reproductive ecology of the species.
SubtypeArticle
KeywordGenomic Library Microsatellites Monopterus Albus Polymorphism
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI10.1111/j.1471-8286.2007.01683.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS IDWOS:000247758100046
WOS KeywordDNA
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8516
Collection期刊论文
Corresponding AuthorTong, J, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Li, W. T.,Liao, X. L.,Yu, X. M.,et al. Isolation and characterization of polymorphic microsatellites in a sex-reversal fish, rice field eel (Monopterus albus)[J]. MOLECULAR ECOLOGY NOTES,2007,7(4):705-707.
APA Li, W. T.,Liao, X. L.,Yu, X. M.,Cheng, L.,Tong, J.,&Tong, J, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2007).Isolation and characterization of polymorphic microsatellites in a sex-reversal fish, rice field eel (Monopterus albus).MOLECULAR ECOLOGY NOTES,7(4),705-707.
MLA Li, W. T.,et al."Isolation and characterization of polymorphic microsatellites in a sex-reversal fish, rice field eel (Monopterus albus)".MOLECULAR ECOLOGY NOTES 7.4(2007):705-707.
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