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Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells
Huang, You-Hua; Huang, Xiao-Hong; Gui, Jian-Fang; Zhang, Qi-Ya; Zhang, QY, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2007-09-01
Source PublicationAPOPTOSIS
ISSN1360-8185
Volume12Issue:9Pages:1569-1577
AbstractA fish cell line, fathead minnow (FHM) cell, was used to investigate the alteration of mitochondrial dynamics and the mechanism of apoptosis under Rana grylio virus (RGV) infection. Microscopy observations, flow-cytometry analysis and molecular marker detection revealed the apoptotic fate of the RGV-infected cells. Some typical apoptotic characteristics, such as chromatin condensation, DNA fragmentation and mitochondrial fragmentation, were observed, and significantly morphological changes of mitochondria, including size, shape, internal structure and distribution, were revealed. The mitochondria in RGV-infected cells were aggregated around the viromatrix, and the aggregation could be blocked by colchicine. Moreover, the Delta psi m collapse was induced, and caspase-9 and caspase-3 were activated in the RGV-infected cells. In addition, NF-kappa B activation and intracellular Ca2+ increase were also detected at different times after infection. The data revealed the detailed dynamics of mitochondrion-mediated apoptosis induced by an iridovirus, and provided the first report on mitochondrial fragmentation during virus-induced apoptosis in fish cells.; A fish cell line, fathead minnow (FHM) cell, was used to investigate the alteration of mitochondrial dynamics and the mechanism of apoptosis under Rana grylio virus (RGV) infection. Microscopy observations, flow-cytometry analysis and molecular marker detection revealed the apoptotic fate of the RGV-infected cells. Some typical apoptotic characteristics, such as chromatin condensation, DNA fragmentation and mitochondrial fragmentation, were observed, and significantly morphological changes of mitochondria, including size, shape, internal structure and distribution, were revealed. The mitochondria in RGV-infected cells were aggregated around the viromatrix, and the aggregation could be blocked by colchicine. Moreover, the Delta psi m collapse was induced, and caspase-9 and caspase-3 were activated in the RGV-infected cells. In addition, NF-kappa B activation and intracellular Ca2+ increase were also detected at different times after infection. The data revealed the detailed dynamics of mitochondrion-mediated apoptosis induced by an iridovirus, and provided the first report on mitochondrial fragmentation during virus-induced apoptosis in fish cells.
SubtypeArticle
KeywordFish Cell Apoptosis Mitochondrion Mitochondrial Fragmentation Rana Glylio Virus (Rgv) Caspase
DepartmentChinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Cell Biology
DOI10.1007/s10495-007-0089-1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000248325200002
WOS KeywordNF-KAPPA-B ; IN-VITRO ; DEATH ; FISSION ; INDUCTION ; NECROSIS ; FUSION ; LINE ; IRIDOVIRUS ; EXPRESSION
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Cited Times:49[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8482
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Huang, You-Hua,Huang, Xiao-Hong,Gui, Jian-Fang,et al. Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells[J]. APOPTOSIS,2007,12(9):1569-1577.
APA Huang, You-Hua,Huang, Xiao-Hong,Gui, Jian-Fang,Zhang, Qi-Ya,&Zhang, QY, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2007).Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells.APOPTOSIS,12(9),1569-1577.
MLA Huang, You-Hua,et al."Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells".APOPTOSIS 12.9(2007):1569-1577.
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