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Isolation and characterization of polymorphic microsatellite loci in Wuchang bream (Megalobrama amblycephala)
Li, W. T.; Liao, X. L.; Yu, X. M.; Wang, D.; Tong, J.; Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-09-01
Source PublicationMOLECULAR ECOLOGY NOTES
ISSN1471-8278
Volume7Issue:5Pages:771-773
AbstractWuchang bream (Megalobrama amblycephala) is an economically important fish in China. From a (GT)(13)-enriched genomic library, 20 microsatellites were developed. Nine of these 20 loci were polymorphic in a test population with allele numbers ranging from two to four, and the observed and expected heterozygosities ranging from 0.2609 to 0.7826 and from 0.3739 to 0.7546, respectively. In the cross-species amplifications, six of these nine loci were also polymorphic in white amur bream (Parabramis pekinensis). These polymorphic microsatellite loci are potentially useful for population genetics of Wuchang bream and its closely related species.; Wuchang bream (Megalobrama amblycephala) is an economically important fish in China. From a (GT)(13)-enriched genomic library, 20 microsatellites were developed. Nine of these 20 loci were polymorphic in a test population with allele numbers ranging from two to four, and the observed and expected heterozygosities ranging from 0.2609 to 0.7826 and from 0.3739 to 0.7546, respectively. In the cross-species amplifications, six of these nine loci were also polymorphic in white amur bream (Parabramis pekinensis). These polymorphic microsatellite loci are potentially useful for population genetics of Wuchang bream and its closely related species.
SubtypeArticle
KeywordCross-species Amplification Genomic Library Megalobrama Amblycephala Microsatellite Parabramis Pekinensis Polymorphism
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI10.1111/j.1471-8286.2007.01689.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS IDWOS:000249193000008
WOS KeywordFISHES ; DNA
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8468
Collection期刊论文
Corresponding AuthorTong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Li, W. T.,Liao, X. L.,Yu, X. M.,et al. Isolation and characterization of polymorphic microsatellite loci in Wuchang bream (Megalobrama amblycephala)[J]. MOLECULAR ECOLOGY NOTES,2007,7(5):771-773.
APA Li, W. T.,Liao, X. L.,Yu, X. M.,Wang, D.,Tong, J.,&Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Isolation and characterization of polymorphic microsatellite loci in Wuchang bream (Megalobrama amblycephala).MOLECULAR ECOLOGY NOTES,7(5),771-773.
MLA Li, W. T.,et al."Isolation and characterization of polymorphic microsatellite loci in Wuchang bream (Megalobrama amblycephala)".MOLECULAR ECOLOGY NOTES 7.5(2007):771-773.
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