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Apoptosis induction on human hepatoma cells Hep G2 of decabrominated diphenyl ether (PBDE-209)
Hu, Xiao-Zhong; Xu, Ying; Hu, De-Cong; Hui, Yang; Yang, Fang-Xing; Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2007-06-15
Source PublicationTOXICOLOGY LETTERS
ISSN0378-4274
Volume171Issue:1-2Pages:19-28
AbstractPolybrominated diphenyl ethers (PBDEs) are an important class of halogenated organic brominated flame retardants. Because of their presence in abiotic and biotic environments widely and their structural similarity to polychlorinated biphenyls (PCBs), concern has been raised on their possible adverse health effects to humans. This study was designed to determine the anti-proliferative, apoptotic properties of decabrominated diphenyl ether (PBDE-209), using a human hepatoma Hep G2 line as a model system. Hep G2 cells were cultured in the presence of PBDE-209 at various concentrations (1.0-100.0 mu mol/L) for 72 h and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The results showed that PBDE-209 inhibited the cells viability in time and concentration-dependent characteristics at concentrations (10.0-100.0 mu mol/L). We found that anti-proliferative effect of PBDE-209 was associated with apoptosis on Hep G2 cells by determinations of morphological changes, cell cycle and apoptosis. Mechanism study showed that PBDE-209 could increase the generation of intracellular reactive oxygen species (ROS) concentration-dependently. Antioxidant N-acetylcyteine partially inhibited the increase of ROS. The mechanism for its hepatoma-inhibitory effects was the induction of cellular apoptosis through ROS generation. In addition, activity of lactate dehydrogenase (LDH) release increased when the cells incubated with PBDE-209 at various concentrations and times. These results suggested that PBDE-209 had the toxicity activity of anti-proliferation and induction of apoptosis in tumor cells in vitro. (c) 2007 Elsevier Ireland Ltd. All rights reserved.; Polybrominated diphenyl ethers (PBDEs) are an important class of halogenated organic brominated flame retardants. Because of their presence in abiotic and biotic environments widely and their structural similarity to polychlorinated biphenyls (PCBs), concern has been raised on their possible adverse health effects to humans. This study was designed to determine the anti-proliferative, apoptotic properties of decabrominated diphenyl ether (PBDE-209), using a human hepatoma Hep G2 line as a model system. Hep G2 cells were cultured in the presence of PBDE-209 at various concentrations (1.0-100.0 mu mol/L) for 72 h and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The results showed that PBDE-209 inhibited the cells viability in time and concentration-dependent characteristics at concentrations (10.0-100.0 mu mol/L). We found that anti-proliferative effect of PBDE-209 was associated with apoptosis on Hep G2 cells by determinations of morphological changes, cell cycle and apoptosis. Mechanism study showed that PBDE-209 could increase the generation of intracellular reactive oxygen species (ROS) concentration-dependently. Antioxidant N-acetylcyteine partially inhibited the increase of ROS. The mechanism for its hepatoma-inhibitory effects was the induction of cellular apoptosis through ROS generation. In addition, activity of lactate dehydrogenase (LDH) release increased when the cells incubated with PBDE-209 at various concentrations and times. These results suggested that PBDE-209 had the toxicity activity of anti-proliferation and induction of apoptosis in tumor cells in vitro. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
SubtypeArticle
KeywordPbde-209 Hep G2 Cells Cell Viability Apoptosis Ldh Release Reactive Oxygen Species
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China; Technol Ctr Hubei Entry Exit Inspect & Quarantine, Wuhan 430022, Peoples R China
Subject AreaToxicology
DOI10.1016/j.toxlet.2007.04.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000248467000003
WOS KeywordBROMINATED FLAME RETARDANTS ; DEVELOPMENTAL EXPOSURE ; HL-60 CELLS ; IN-VITRO ; NEONATAL EXPOSURE ; ADIPOSE-TISSUE ; RAT ; MOUSE ; DEATH ; ENVIRONMENT
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8444
Collection期刊论文
Corresponding AuthorXu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Technol Ctr Hubei Entry Exit Inspect & Quarantine, Wuhan 430022, Peoples R China
Recommended Citation
GB/T 7714
Hu, Xiao-Zhong,Xu, Ying,Hu, De-Cong,et al. Apoptosis induction on human hepatoma cells Hep G2 of decabrominated diphenyl ether (PBDE-209)[J]. TOXICOLOGY LETTERS,2007,171(1-2):19-28.
APA Hu, Xiao-Zhong,Xu, Ying,Hu, De-Cong,Hui, Yang,Yang, Fang-Xing,&Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Apoptosis induction on human hepatoma cells Hep G2 of decabrominated diphenyl ether (PBDE-209).TOXICOLOGY LETTERS,171(1-2),19-28.
MLA Hu, Xiao-Zhong,et al."Apoptosis induction on human hepatoma cells Hep G2 of decabrominated diphenyl ether (PBDE-209)".TOXICOLOGY LETTERS 171.1-2(2007):19-28.
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