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Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection
Lu, Ai-Jun; Dong, Cai-Wen; Du, Chang-Sheng; Zhang, Qi-Ya; Zhang, QY, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China
2007-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume23Issue:3Pages:601-613
AbstractVoltage-dependent anion channel (VDAC, also known as mitochondrial porin) is acknowledged to play an important role in stress-induced mammalian apoptosis. In this study, Paralichthys olivaceus VDAC (PoVDAC) gene was identified as a virally induced gene from Scophthalmus Maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). The full length of PoVDAC cDNA is 1380 bp with an open reading frame of 852 bp encoding a 283 amino acid protein. The deduced PoVDAC contains one alpha-helix, 13 transmembrane beta-strands and one eukaryotic mitochondrial porin signature motif. Constitutive expression of PoVDAC was confirmed in all tested tissues by real-time PCR. Further expression analysis revealed PoVDAC mRNA was upregulated by viral infection. We prepared fish antiserum against recombinant VDAC proteins and detected the PoVDAC in heart lysates from flounder as a 32 kDa band on western blot. Overexpression of PoVDAC in fish cells induced apoptosis. Immunofluoresence localization indicated that the significant distribution changes of PoVDAC have occurred in virus-induced apoptotic cells. This is the first report on the inductive expression of VDAC by viral infection, suggesting that PoVDAC might be mediated flounder antiviral immune response through induction of apoptosis. (c) 2007 Elsevier Ltd. All rights reserved.; Voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is acknowledged to play an important role in stress-induced mammalian apoptosis. In this study, Paralichthys olivaceus VDAC (PoVDAC) gene was identified as a virally induced gene from Scophthalmus Maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). The full length of PoVDAC cDNA is 1380 bp with an open reading frame of 852 bp encoding a 283 amino acid protein. The deduced PoVDAC contains one alpha-helix, 13 transmembrane beta-strands and one eukaryotic mitochondrial porin signature motif. Constitutive expression of PoVDAC was confirmed in all tested tissues by real-time PCR. Further expression analysis revealed PoVDAC mRNA was upregulated by viral infection. We prepared fish antiserum against recombinant VDAC proteins and detected the PoVDAC in heart lysates from flounder as a 32 kDa band on western blot. Overexpression of PoVDAC in fish cells induced apoptosis. Immunofluoresence localization indicated that the significant distribution changes of PoVDAC have occurred in virus-induced apoptotic cells. This is the first report on the inductive expression of VDAC by viral infection, suggesting that PoVDAC might be mediated flounder antiviral immune response through induction of apoptosis. (c) 2007 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordVoltage-dependent Anion Channel Inductive Expression Paralichthys Olivaceus Virus Infection Apoptosis
DepartmentGrad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China; Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2007.01.007
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000248509800011
WOS KeywordCELL-DEATH ; DROSOPHILA-MELANOGASTER ; CYTOCHROME-C ; APOPTOSIS ; IDENTIFICATION ; MITOCHONDRIA ; PROTEINS ; FAMILY ; LOCALIZATION ; ACTIVATION
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8438
Collection期刊论文
Corresponding AuthorZhang, QY, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China
Affiliation1.Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China
2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China
Recommended Citation
GB/T 7714
Lu, Ai-Jun,Dong, Cai-Wen,Du, Chang-Sheng,et al. Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2007,23(3):601-613.
APA Lu, Ai-Jun,Dong, Cai-Wen,Du, Chang-Sheng,Zhang, Qi-Ya,&Zhang, QY, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China.(2007).Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection.FISH & SHELLFISH IMMUNOLOGY,23(3),601-613.
MLA Lu, Ai-Jun,et al."Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection".FISH & SHELLFISH IMMUNOLOGY 23.3(2007):601-613.
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