| Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection | |
| Lu, Ai-Jun; Dong, Cai-Wen; Du, Chang-Sheng; Zhang, Qi-Ya; Zhang, QY, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China | |
| 2007-09-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 23Issue:3Pages:601-613 |
| Abstract | Voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is acknowledged to play an important role in stress-induced mammalian apoptosis. In this study, Paralichthys olivaceus VDAC (PoVDAC) gene was identified as a virally induced gene from Scophthalmus Maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). The full length of PoVDAC cDNA is 1380 bp with an open reading frame of 852 bp encoding a 283 amino acid protein. The deduced PoVDAC contains one alpha-helix, 13 transmembrane beta-strands and one eukaryotic mitochondrial porin signature motif. Constitutive expression of PoVDAC was confirmed in all tested tissues by real-time PCR. Further expression analysis revealed PoVDAC mRNA was upregulated by viral infection. We prepared fish antiserum against recombinant VDAC proteins and detected the PoVDAC in heart lysates from flounder as a 32 kDa band on western blot. Overexpression of PoVDAC in fish cells induced apoptosis. Immunofluoresence localization indicated that the significant distribution changes of PoVDAC have occurred in virus-induced apoptotic cells. This is the first report on the inductive expression of VDAC by viral infection, suggesting that PoVDAC might be mediated flounder antiviral immune response through induction of apoptosis. (c) 2007 Elsevier Ltd. All rights reserved.; Voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is acknowledged to play an important role in stress-induced mammalian apoptosis. In this study, Paralichthys olivaceus VDAC (PoVDAC) gene was identified as a virally induced gene from Scophthalmus Maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). The full length of PoVDAC cDNA is 1380 bp with an open reading frame of 852 bp encoding a 283 amino acid protein. The deduced PoVDAC contains one alpha-helix, 13 transmembrane beta-strands and one eukaryotic mitochondrial porin signature motif. Constitutive expression of PoVDAC was confirmed in all tested tissues by real-time PCR. Further expression analysis revealed PoVDAC mRNA was upregulated by viral infection. We prepared fish antiserum against recombinant VDAC proteins and detected the PoVDAC in heart lysates from flounder as a 32 kDa band on western blot. Overexpression of PoVDAC in fish cells induced apoptosis. Immunofluoresence localization indicated that the significant distribution changes of PoVDAC have occurred in virus-induced apoptotic cells. This is the first report on the inductive expression of VDAC by viral infection, suggesting that PoVDAC might be mediated flounder antiviral immune response through induction of apoptosis. (c) 2007 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Voltage-dependent Anion Channel Inductive Expression Paralichthys Olivaceus Virus Infection Apoptosis |
| Department | Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China; Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China |
| Subject Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2007.01.007 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000248509800011 |
| WOS Keyword | CELL-DEATH ; DROSOPHILA-MELANOGASTER ; CYTOCHROME-C ; APOPTOSIS ; IDENTIFICATION ; MITOCHONDRIA ; PROTEINS ; FAMILY ; LOCALIZATION ; ACTIVATION |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8438 |
| Collection | 期刊论文 |
| Corresponding Author | Zhang, QY, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China |
| Affiliation | 1.Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China 2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China |
| Recommended Citation GB/T 7714 | Lu, Ai-Jun,Dong, Cai-Wen,Du, Chang-Sheng,et al. Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2007,23(3):601-613. |
| APA | Lu, Ai-Jun,Dong, Cai-Wen,Du, Chang-Sheng,Zhang, Qi-Ya,&Zhang, QY, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China.(2007).Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection.FISH & SHELLFISH IMMUNOLOGY,23(3),601-613. |
| MLA | Lu, Ai-Jun,et al."Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection".FISH & SHELLFISH IMMUNOLOGY 23.3(2007):601-613. |
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