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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection
作者: Lu, Ai-Jun; Dong, Cai-Wen; Du, Chang-Sheng; Zhang, Qi-Ya
通讯作者: Zhang, QY, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China
关键词: voltage-dependent anion channel ; inductive expression ; Paralichthys olivaceus ; virus infection ; apoptosis
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2007-09-01
DOI: 10.1016/j.fsi.2007.01.007
卷: 23, 期:3, 页:601-613
收录类别: SCI
文章类型: Article
部门归属: Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China; Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: Voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is acknowledged to play an important role in stress-induced mammalian apoptosis. In this study, Paralichthys olivaceus VDAC (PoVDAC) gene was identified as a virally induced gene from Scophthalmus Maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). The full length of PoVDAC cDNA is 1380 bp with an open reading frame of 852 bp encoding a 283 amino acid protein. The deduced PoVDAC contains one alpha-helix, 13 transmembrane beta-strands and one eukaryotic mitochondrial porin signature motif. Constitutive expression of PoVDAC was confirmed in all tested tissues by real-time PCR. Further expression analysis revealed PoVDAC mRNA was upregulated by viral infection. We prepared fish antiserum against recombinant VDAC proteins and detected the PoVDAC in heart lysates from flounder as a 32 kDa band on western blot. Overexpression of PoVDAC in fish cells induced apoptosis. Immunofluoresence localization indicated that the significant distribution changes of PoVDAC have occurred in virus-induced apoptotic cells. This is the first report on the inductive expression of VDAC by viral infection, suggesting that PoVDAC might be mediated flounder antiviral immune response through induction of apoptosis. (c) 2007 Elsevier Ltd. All rights reserved.
英文摘要: Voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is acknowledged to play an important role in stress-induced mammalian apoptosis. In this study, Paralichthys olivaceus VDAC (PoVDAC) gene was identified as a virally induced gene from Scophthalmus Maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). The full length of PoVDAC cDNA is 1380 bp with an open reading frame of 852 bp encoding a 283 amino acid protein. The deduced PoVDAC contains one alpha-helix, 13 transmembrane beta-strands and one eukaryotic mitochondrial porin signature motif. Constitutive expression of PoVDAC was confirmed in all tested tissues by real-time PCR. Further expression analysis revealed PoVDAC mRNA was upregulated by viral infection. We prepared fish antiserum against recombinant VDAC proteins and detected the PoVDAC in heart lysates from flounder as a 32 kDa band on western blot. Overexpression of PoVDAC in fish cells induced apoptosis. Immunofluoresence localization indicated that the significant distribution changes of PoVDAC have occurred in virus-induced apoptotic cells. This is the first report on the inductive expression of VDAC by viral infection, suggesting that PoVDAC might be mediated flounder antiviral immune response through induction of apoptosis. (c) 2007 Elsevier Ltd. All rights reserved.
关键词[WOS]: CELL-DEATH ; DROSOPHILA-MELANOGASTER ; CYTOCHROME-C ; APOPTOSIS ; IDENTIFICATION ; MITOCHONDRIA ; PROTEINS ; FAMILY ; LOCALIZATION ; ACTIVATION
语种: 英语
WOS记录号: WOS:000248509800011
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8438
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China
2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China

Recommended Citation:
Lu, Ai-Jun; Dong, Cai-Wen; Du, Chang-Sheng; Zhang, Qi-Ya.Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection,FISH & SHELLFISH IMMUNOLOGY,2007,23(3):601-613
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