中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
学科主题: Biochemistry & Molecular Biology; Neurosciences
题名: Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)
作者: Zhou, L.; Li, C. J.; Wang, Y.; Xia, W.; Yao, B.; Jin, J. Y.; Gui, J. F.
通讯作者: Gui, JF, Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
关键词: myelin basic protein ; orange-spotted grouper ; hypothalamus ; oligodendrocyte
刊名: JOURNAL OF CHEMICAL NEUROANATOMY
发表日期: 2007-09-01
DOI: 10.1016/j.jchemneu.2007.03.011
卷: 34, 期:1-2, 页:47-59
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]: Biochemistry & Molecular Biology ; Neurosciences & Neurology
摘要: Myelin basic protein (MBP), as a major component of the myelin sheath, has been revealed to play an important role informing and maintaining myelin structure in vertebrate nervous system. In teleost, hypothalamus is an instinctive brain center and plays significant roles in many physiological functions, such as energy metabolism, growth, reproduction, and stress response. In comparison with other MBP identified in vertebrates, a smallest MBP is cloned and identified from the orange-spotted grouper hypothalamic cDNA plasmid library in this study. RT-PCR analysis and Western blot detection indicate that the EcMBP is specific to hypothalamus, and expresses mainly in the tuberal hypothalamus in adult grouper. Immunofluorescence localization suggests that EcMBP should be expressed by oligodendrocytes, and the expressing cells should be concentrated in hypothalamus and the area surrounding hypothalamus, such as NPOpc, VC, DP, NLTm, and NDLI The studies on EcMBP expression pattern and developmental behaviour in the brains of grouper embryos and larvae reveal that the EcMBP-expressing cells are only limited in a defined set of cells on the border of hypothalamus, and suggest that the EcMBP-expressing cells might be a subpopulation of oliaodendrocyte progenitor cells. This study not only identifies a smallest MBP isoform specific to hypothalamus that can be used as a molecular marker of oligodendrocytes in fish, but also provides new insights for MBP evolution and cellular distribution. (C) 2007 Elsevier B.V.. All rights reserved.
英文摘要: Myelin basic protein (MBP), as a major component of the myelin sheath, has been revealed to play an important role informing and maintaining myelin structure in vertebrate nervous system. In teleost, hypothalamus is an instinctive brain center and plays significant roles in many physiological functions, such as energy metabolism, growth, reproduction, and stress response. In comparison with other MBP identified in vertebrates, a smallest MBP is cloned and identified from the orange-spotted grouper hypothalamic cDNA plasmid library in this study. RT-PCR analysis and Western blot detection indicate that the EcMBP is specific to hypothalamus, and expresses mainly in the tuberal hypothalamus in adult grouper. Immunofluorescence localization suggests that EcMBP should be expressed by oligodendrocytes, and the expressing cells should be concentrated in hypothalamus and the area surrounding hypothalamus, such as NPOpc, VC, DP, NLTm, and NDLI The studies on EcMBP expression pattern and developmental behaviour in the brains of grouper embryos and larvae reveal that the EcMBP-expressing cells are only limited in a defined set of cells on the border of hypothalamus, and suggest that the EcMBP-expressing cells might be a subpopulation of oliaodendrocyte progenitor cells. This study not only identifies a smallest MBP isoform specific to hypothalamus that can be used as a molecular marker of oligodendrocytes in fish, but also provides new insights for MBP evolution and cellular distribution. (C) 2007 Elsevier B.V.. All rights reserved.
关键词[WOS]: MYELIN-BASIC-PROTEIN ; RAT PANCREATIC-ISLETS ; GONADOTROPIN-RELEASING-HORMONE ; CENTRAL-NERVOUS-SYSTEM ; MOLECULAR-CLONING ; GOLDFISH BRAIN ; SEX REVERSAL ; OLIGODENDROCYTE SPECIFICATION ; DIFFERENTIAL EXPRESSION ; SCYLIORHINUS-TORAZAME
语种: 英语
WOS记录号: WOS:000248494100004
ISSN号: 0891-0618
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8434
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides).pdf(2227KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhou, L.; Li, C. J.; Wang, Y.; Xia, W.; Yao, B.; Jin, J. Y.; Gui, J. F..Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides),JOURNAL OF CHEMICAL NEUROANATOMY,2007,34(1-2):47-59
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, L.]'s Articles
[Li, C. J.]'s Articles
[Wang, Y.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, L.]‘s Articles
[Li, C. J.]‘s Articles
[Wang, Y.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides).pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace