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Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)
Zhou, L.; Li, C. J.; Wang, Y.; Xia, W.; Yao, B.; Jin, J. Y.; Gui, J. F.; Gui, JF, Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
2007-09-01
Source PublicationJOURNAL OF CHEMICAL NEUROANATOMY
ISSN0891-0618
Volume34Issue:1-2Pages:47-59
AbstractMyelin basic protein (MBP), as a major component of the myelin sheath, has been revealed to play an important role informing and maintaining myelin structure in vertebrate nervous system. In teleost, hypothalamus is an instinctive brain center and plays significant roles in many physiological functions, such as energy metabolism, growth, reproduction, and stress response. In comparison with other MBP identified in vertebrates, a smallest MBP is cloned and identified from the orange-spotted grouper hypothalamic cDNA plasmid library in this study. RT-PCR analysis and Western blot detection indicate that the EcMBP is specific to hypothalamus, and expresses mainly in the tuberal hypothalamus in adult grouper. Immunofluorescence localization suggests that EcMBP should be expressed by oligodendrocytes, and the expressing cells should be concentrated in hypothalamus and the area surrounding hypothalamus, such as NPOpc, VC, DP, NLTm, and NDLI The studies on EcMBP expression pattern and developmental behaviour in the brains of grouper embryos and larvae reveal that the EcMBP-expressing cells are only limited in a defined set of cells on the border of hypothalamus, and suggest that the EcMBP-expressing cells might be a subpopulation of oliaodendrocyte progenitor cells. This study not only identifies a smallest MBP isoform specific to hypothalamus that can be used as a molecular marker of oligodendrocytes in fish, but also provides new insights for MBP evolution and cellular distribution. (C) 2007 Elsevier B.V.. All rights reserved.; Myelin basic protein (MBP), as a major component of the myelin sheath, has been revealed to play an important role informing and maintaining myelin structure in vertebrate nervous system. In teleost, hypothalamus is an instinctive brain center and plays significant roles in many physiological functions, such as energy metabolism, growth, reproduction, and stress response. In comparison with other MBP identified in vertebrates, a smallest MBP is cloned and identified from the orange-spotted grouper hypothalamic cDNA plasmid library in this study. RT-PCR analysis and Western blot detection indicate that the EcMBP is specific to hypothalamus, and expresses mainly in the tuberal hypothalamus in adult grouper. Immunofluorescence localization suggests that EcMBP should be expressed by oligodendrocytes, and the expressing cells should be concentrated in hypothalamus and the area surrounding hypothalamus, such as NPOpc, VC, DP, NLTm, and NDLI The studies on EcMBP expression pattern and developmental behaviour in the brains of grouper embryos and larvae reveal that the EcMBP-expressing cells are only limited in a defined set of cells on the border of hypothalamus, and suggest that the EcMBP-expressing cells might be a subpopulation of oliaodendrocyte progenitor cells. This study not only identifies a smallest MBP isoform specific to hypothalamus that can be used as a molecular marker of oligodendrocytes in fish, but also provides new insights for MBP evolution and cellular distribution. (C) 2007 Elsevier B.V.. All rights reserved.
SubtypeArticle
KeywordMyelin Basic Protein Orange-spotted Grouper Hypothalamus Oligodendrocyte
DepartmentChinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Neurosciences
DOI10.1016/j.jchemneu.2007.03.011
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Neurosciences & Neurology
WOS SubjectBiochemistry & Molecular Biology ; Neurosciences
WOS IDWOS:000248494100004
WOS KeywordMYELIN-BASIC-PROTEIN ; RAT PANCREATIC-ISLETS ; GONADOTROPIN-RELEASING-HORMONE ; CENTRAL-NERVOUS-SYSTEM ; MOLECULAR-CLONING ; GOLDFISH BRAIN ; SEX REVERSAL ; OLIGODENDROCYTE SPECIFICATION ; DIFFERENTIAL EXPRESSION ; SCYLIORHINUS-TORAZAME
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8434
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, L.,Li, C. J.,Wang, Y.,et al. Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)[J]. JOURNAL OF CHEMICAL NEUROANATOMY,2007,34(1-2):47-59.
APA Zhou, L..,Li, C. J..,Wang, Y..,Xia, W..,Yao, B..,...&Gui, JF, Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China.(2007).Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides).JOURNAL OF CHEMICAL NEUROANATOMY,34(1-2),47-59.
MLA Zhou, L.,et al."Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides)".JOURNAL OF CHEMICAL NEUROANATOMY 34.1-2(2007):47-59.
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