| Antisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio) | |
| Hu, Wei; Li, Shuangfei; Tang, Bin; Wang, Yaping; Lin, Haoran; Liu, Xiaochun; Zou, Jun; Zhu, Zuoyan; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2007-10-03 | |
| Source Publication | AQUACULTURE
 |
| ISSN | 0044-8486 |
| Volume | 271Issue:1-4Pages:498-506 |
| Abstract | Generating transgenic fish with desirable traits (e.g., rapid growth, larger size, etc.) for commercial use has been hampered by concerns for biosafety and competition if these fish are released into the environment. These obstacles may be overcome by producing transgenic fish that are sterile, possibly by inhibiting hormones related to reproduction. In vertebrates, synthesis and release of gonadotropin (GtH) and other reproductive hormones is mediated by gonadotropin-releasing hormone (GnRH). Recently two cDNA sequences encoding salmon-type GnRH (sGnRH) decapeptides were cloned from common carp (Cyprinus carpio). This study analyzed the expression of these two genes using real-time polymerase chain reaction (RT-PCR) in different tissues carp at varying developmental stages. Transcripts of both genes were detected in ovary and testis in mature and regressed, but not in juvenile carp. To evaluate the effects of sGnRH inhibition, the recombinant gene CAsGnRHpc-antisense, expressing antisense sGnRH RNA driven by a carp beta-actin promoter, was constructed. Blocking sGnRH expression using antisense sGnRH significantly decreased GtH in the blood of male transgenic carp. Furthermore, some antisense transgenic fish had no gonadal development and were completely sterile. These data demonstrate that sGnRH is important for GtH synthesis and development of reproductive organs in carp. Also, the antisense sGnRH strategy may prove effective in generating sterile transgenic fish, eliminating environmental concerns these fish may raise. (c) 2007 Published by Elsevier B.V.; Generating transgenic fish with desirable traits (e.g., rapid growth, larger size, etc.) for commercial use has been hampered by concerns for biosafety and competition if these fish are released into the environment. These obstacles may be overcome by producing transgenic fish that are sterile, possibly by inhibiting hormones related to reproduction. In vertebrates, synthesis and release of gonadotropin (GtH) and other reproductive hormones is mediated by gonadotropin-releasing hormone (GnRH). Recently two cDNA sequences encoding salmon-type GnRH (sGnRH) decapeptides were cloned from common carp (Cyprinus carpio). This study analyzed the expression of these two genes using real-time polymerase chain reaction (RT-PCR) in different tissues carp at varying developmental stages. Transcripts of both genes were detected in ovary and testis in mature and regressed, but not in juvenile carp. To evaluate the effects of sGnRH inhibition, the recombinant gene CAsGnRHpc-antisense, expressing antisense sGnRH RNA driven by a carp beta-actin promoter, was constructed. Blocking sGnRH expression using antisense sGnRH significantly decreased GtH in the blood of male transgenic carp. Furthermore, some antisense transgenic fish had no gonadal development and were completely sterile. These data demonstrate that sGnRH is important for GtH synthesis and development of reproductive organs in carp. Also, the antisense sGnRH strategy may prove effective in generating sterile transgenic fish, eliminating environmental concerns these fish may raise. (c) 2007 Published by Elsevier B.V. |
| Subtype | Article |
| Keyword | Common Carp (Cyprinus Carpio) Gonadotropin-releasing Hormone (Gnrh) Transgene |
| Department | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Zhongshan Univ, Coll Life Sci, Guangzhou 510275, Peoples R China; Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland |
| Subject Area | Fisheries ; Marine & Freshwater Biology |
| DOI | 10.1016/j.aquaculture.2007.04.075 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000249874600052 |
| WOS Keyword | CHICKEN GNRH-II ; GOLDFISH CARASSIUS-AURATUS ; CTENOPHARYNGODON-IDELLUS ; MISGURNUS-MIZOLEPIS ; ACCELERATED-GROWTH ; SALMON GNRH ; MUD LOACH ; FISH ; GENE ; EXPRESSION |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8404 |
| Collection | 期刊论文 |
| Corresponding Author | Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Zhongshan Univ, Coll Life Sci, Guangzhou 510275, Peoples R China 3.Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland |
| Recommended Citation GB/T 7714 | Hu, Wei,Li, Shuangfei,Tang, Bin,et al. Antisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio)[J]. AQUACULTURE,2007,271(1-4):498-506. |
| APA | Hu, Wei.,Li, Shuangfei.,Tang, Bin.,Wang, Yaping.,Lin, Haoran.,...&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2007).Antisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio).AQUACULTURE,271(1-4),498-506. |
| MLA | Hu, Wei,et al."Antisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio)".AQUACULTURE 271.1-4(2007):498-506. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Antisense for gonado（957KB） | 开放获取 | -- | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment