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Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio
Wang, Y.; Hu, W.; Liu, W.-Y.; Wang, Y.-P.; Zhu, Z.-Y.; Hu, W, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China
2007-10-01
Source PublicationJOURNAL OF FISH BIOLOGY
ISSN0022-1112
Volume71Issue:4Pages:1082-1094
AbstractIn this study, an alternative splicing transcript GtH-alpha 291 was identified by RT-PCR, which is 291 nt and exists not only in the pituitary but also in the ovary in common carp Cyprinus carpio. The analysis of GtH-alpha 291 amino acid sequence by the SignalP server predicted that the 'missing segment' might characterize as a signal peptide. In the secretion experiment, GtH-alpha 357 subunit could be secreted out of HeLa cells while GtH-alpha 291 could not, which confirmed the prediction. Co-immunoprecipitation assay proved that GtH-alpha 291 subunit is able to interact with both FSH-beta and LH-beta as GtH-alpha 357 does. This is the first report concerning an alternative splicing transcript of a GtH alpha subunit. Further studies are necessary to elucidate the specific role of this variant in the regulation of gonadal development and sexual maturation. (c) 2007 The Authors.; In this study, an alternative splicing transcript GtH-alpha 291 was identified by RT-PCR, which is 291 nt and exists not only in the pituitary but also in the ovary in common carp Cyprinus carpio. The analysis of GtH-alpha 291 amino acid sequence by the SignalP server predicted that the 'missing segment' might characterize as a signal peptide. In the secretion experiment, GtH-alpha 357 subunit could be secreted out of HeLa cells while GtH-alpha 291 could not, which confirmed the prediction. Co-immunoprecipitation assay proved that GtH-alpha 291 subunit is able to interact with both FSH-beta and LH-beta as GtH-alpha 357 does. This is the first report concerning an alternative splicing transcript of a GtH alpha subunit. Further studies are necessary to elucidate the specific role of this variant in the regulation of gonadal development and sexual maturation. (c) 2007 The Authors.
SubtypeArticle
KeywordAlternative Splicing Common Carp Gonadotropin Signal Peptide
DepartmentChinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1111/j.1095-8649.2007.01582.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000250265800010
WOS KeywordPITUITARY GLYCOPROTEIN HORMONES ; FOLLICLE-STIMULATING-HORMONE ; PRE-MESSENGER-RNA ; ENCODING 2 TYPES ; SALMON GONADOTROPINS ; NUCLEOTIDE-SEQUENCE ; MOLECULAR-CLONING ; SIGNAL PEPTIDES ; RECEPTOR GENE ; EXPRESSION
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8392
Collection期刊论文
Corresponding AuthorHu, W, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Y.,Hu, W.,Liu, W.-Y.,et al. Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio[J]. JOURNAL OF FISH BIOLOGY,2007,71(4):1082-1094.
APA Wang, Y.,Hu, W.,Liu, W.-Y.,Wang, Y.-P.,Zhu, Z.-Y.,&Hu, W, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China.(2007).Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio.JOURNAL OF FISH BIOLOGY,71(4),1082-1094.
MLA Wang, Y.,et al."Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio".JOURNAL OF FISH BIOLOGY 71.4(2007):1082-1094.
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