中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
学科主题: Fisheries; Marine & Freshwater Biology
题名: Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio
作者: Wang, Y.; Hu, W.; Liu, W.-Y.; Wang, Y.-P.; Zhu, Z.-Y.
通讯作者: Hu, W, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China
关键词: alternative splicing ; common carp ; gonadotropin ; signal peptide
刊名: JOURNAL OF FISH BIOLOGY
发表日期: 2007-10-01
DOI: 10.1111/j.1095-8649.2007.01582.x
卷: 71, 期:4, 页:1082-1094
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
摘要: In this study, an alternative splicing transcript GtH-alpha 291 was identified by RT-PCR, which is 291 nt and exists not only in the pituitary but also in the ovary in common carp Cyprinus carpio. The analysis of GtH-alpha 291 amino acid sequence by the SignalP server predicted that the 'missing segment' might characterize as a signal peptide. In the secretion experiment, GtH-alpha 357 subunit could be secreted out of HeLa cells while GtH-alpha 291 could not, which confirmed the prediction. Co-immunoprecipitation assay proved that GtH-alpha 291 subunit is able to interact with both FSH-beta and LH-beta as GtH-alpha 357 does. This is the first report concerning an alternative splicing transcript of a GtH alpha subunit. Further studies are necessary to elucidate the specific role of this variant in the regulation of gonadal development and sexual maturation. (c) 2007 The Authors.
英文摘要: In this study, an alternative splicing transcript GtH-alpha 291 was identified by RT-PCR, which is 291 nt and exists not only in the pituitary but also in the ovary in common carp Cyprinus carpio. The analysis of GtH-alpha 291 amino acid sequence by the SignalP server predicted that the 'missing segment' might characterize as a signal peptide. In the secretion experiment, GtH-alpha 357 subunit could be secreted out of HeLa cells while GtH-alpha 291 could not, which confirmed the prediction. Co-immunoprecipitation assay proved that GtH-alpha 291 subunit is able to interact with both FSH-beta and LH-beta as GtH-alpha 357 does. This is the first report concerning an alternative splicing transcript of a GtH alpha subunit. Further studies are necessary to elucidate the specific role of this variant in the regulation of gonadal development and sexual maturation. (c) 2007 The Authors.
关键词[WOS]: PITUITARY GLYCOPROTEIN HORMONES ; FOLLICLE-STIMULATING-HORMONE ; PRE-MESSENGER-RNA ; ENCODING 2 TYPES ; SALMON GONADOTROPINS ; NUCLEOTIDE-SEQUENCE ; MOLECULAR-CLONING ; SIGNAL PEPTIDES ; RECEPTOR GENE ; EXPRESSION
语种: 英语
WOS记录号: WOS:000250265800010
ISSN号: 0022-1112
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8392
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio.pdf(568KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Wang, Y.; Hu, W.; Liu, W.-Y.; Wang, Y.-P.; Zhu, Z.-Y..Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio,JOURNAL OF FISH BIOLOGY,2007,71(4):1082-1094
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Y.]'s Articles
[Hu, W.]'s Articles
[Liu, W.-Y.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Y.]‘s Articles
[Hu, W.]‘s Articles
[Liu, W.-Y.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace