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Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC
Fang, Huaifang; Yang, Fangxing; Sun, Junling; Zeng, Zhaorui; Xu, Ying; Zeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2007-10-01
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume28Issue:20Pages:3697-3704
AbstractThis paper describes a novel method that applies pressure-assisted field-amplified sample injection with reverse migrating micelles (PA-FASI-RMM) for the online concentration of neutral analytes in MEKC with a low-pH BGE. After injection of a plug of water into the separation capillary, negative voltage and positive pressure were simultaneously applied to initialize PA-FASI-RMM injection. The hydrodynamic flow generated by the positive pressure compensated the reverse EOF in the water plug and allowed the water plug to remain in the capillary during FASI with reverse migrating micelles (FASI-RMM) to obtain a much longer injection time than usual, which improved stacking efficiency greatly. Equations describing this injection mode were introduced and were supported by experimental results. For a 450-s online PA-FASI-RMM injection, three orders of magnitude sample enhancement in terms of peak area could be observed for the steroids and an achievement of detection limits was between 1 and 10 ng/mL.; This paper describes a novel method that applies pressure-assisted field-amplified sample injection with reverse migrating micelles (PA-FASI-RMM) for the online concentration of neutral analytes in MEKC with a low-pH BGE. After injection of a plug of water into the separation capillary, negative voltage and positive pressure were simultaneously applied to initialize PA-FASI-RMM injection. The hydrodynamic flow generated by the positive pressure compensated the reverse EOF in the water plug and allowed the water plug to remain in the capillary during FASI with reverse migrating micelles (FASI-RMM) to obtain a much longer injection time than usual, which improved stacking efficiency greatly. Equations describing this injection mode were introduced and were supported by experimental results. For a 450-s online PA-FASI-RMM injection, three orders of magnitude sample enhancement in terms of peak area could be observed for the steroids and an achievement of detection limits was between 1 and 10 ng/mL.
SubtypeArticle
KeywordMekc Neutral Analyte Pressure-assisted Field-amplified Sample Injection
DepartmentWuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; Chinese Acad Sci, State Key Lab Freshwater Ecol, Wuhan, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Subject AreaAnalytical ; Biochemical Research Methods ; Chemistry
DOI10.1002/elps.200700210
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000250657100014
WOS KeywordCAPILLARY-ZONE-ELECTROPHORESIS ; SELECTIVE EXHAUSTIVE INJECTION ; STACKING NEUTRAL ANALYTES ; ELECTROKINETIC CHROMATOGRAPHY ; ONLINE CONCENTRATION ; HIGH-SALT ; OPTIMIZATION ; SPECTROMETRY ; ENHANCEMENT ; SEPARATION
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Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8386
Collection期刊论文
Corresponding AuthorZeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
Affiliation1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol, Wuhan, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Fang, Huaifang,Yang, Fangxing,Sun, Junling,et al. Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC[J]. ELECTROPHORESIS,2007,28(20):3697-3704.
APA Fang, Huaifang,Yang, Fangxing,Sun, Junling,Zeng, Zhaorui,Xu, Ying,&Zeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.(2007).Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC.ELECTROPHORESIS,28(20),3697-3704.
MLA Fang, Huaifang,et al."Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC".ELECTROPHORESIS 28.20(2007):3697-3704.
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