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Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis
Que, Yanfu; Zhu, Bin; Rosenthal, H.; Chang, Jianbo; Chang, JB, Chinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430079, Peoples R China
2007-11-01
Source PublicationMOLECULAR ECOLOGY NOTES
ISSN1471-8278
Volume7Issue:6Pages:1265-1267
AbstractFifteen polymorphic microsatellite loci were developed for the Chinese soft-shelled turtle (Pelodiscus sinensis) from the (GT)(n) microsatellite-enriched genomic library, using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol. The polymorphism of all 15 loci ranged from two to seven alleles with observed heterozygosities ranging from 0.03 to 0.98 (mean 0.43) in one population of 40 individuals. These novel loci will be helpful for understanding the population structure at genetic level and marker-assisted breeding of this vulnerable species.; Fifteen polymorphic microsatellite loci were developed for the Chinese soft-shelled turtle (Pelodiscus sinensis) from the (GT)(n) microsatellite-enriched genomic library, using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol. The polymorphism of all 15 loci ranged from two to seven alleles with observed heterozygosities ranging from 0.03 to 0.98 (mean 0.43) in one population of 40 individuals. These novel loci will be helpful for understanding the population structure at genetic level and marker-assisted breeding of this vulnerable species.
SubtypeArticle
KeywordMicrosatellites Pelodiscus Sinensis Polymorphism
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430079, Peoples R China; Minist Water Resources, Inst Hydroecol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Wuhan 430072, Peoples R China; World Sturgeon Conservat Soc, D-21629 Neu Wulmstorf, Germany; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI10.1111/j.1471-8286.2007.01850.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS IDWOS:000250647700095
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8376
Collection期刊论文
Corresponding AuthorChang, JB, Chinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430079, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430079, Peoples R China
2.Minist Water Resources, Inst Hydroecol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Wuhan 430072, Peoples R China
4.World Sturgeon Conservat Soc, D-21629 Neu Wulmstorf, Germany
5.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Que, Yanfu,Zhu, Bin,Rosenthal, H.,et al. Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis[J]. MOLECULAR ECOLOGY NOTES,2007,7(6):1265-1267.
APA Que, Yanfu,Zhu, Bin,Rosenthal, H.,Chang, Jianbo,&Chang, JB, Chinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430079, Peoples R China.(2007).Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis.MOLECULAR ECOLOGY NOTES,7(6),1265-1267.
MLA Que, Yanfu,et al."Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis".MOLECULAR ECOLOGY NOTES 7.6(2007):1265-1267.
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