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学科主题: Biochemistry & Molecular Biology; Ecology; Evolutionary Biology
题名: Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis
作者: Que, Yanfu; Zhu, Bin; Rosenthal, H.; Chang, Jianbo
通讯作者: Chang, JB, Chinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430079, Peoples R China
关键词: microsatellites ; Pelodiscus sinensis ; polymorphism
刊名: MOLECULAR ECOLOGY NOTES
发表日期: 2007-11-01
DOI: 10.1111/j.1471-8286.2007.01850.x
卷: 7, 期:6, 页:1265-1267
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430079, Peoples R China; Minist Water Resources, Inst Hydroecol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Wuhan 430072, Peoples R China; World Sturgeon Conservat Soc, D-21629 Neu Wulmstorf, Germany; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
摘要: Fifteen polymorphic microsatellite loci were developed for the Chinese soft-shelled turtle (Pelodiscus sinensis) from the (GT)(n) microsatellite-enriched genomic library, using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol. The polymorphism of all 15 loci ranged from two to seven alleles with observed heterozygosities ranging from 0.03 to 0.98 (mean 0.43) in one population of 40 individuals. These novel loci will be helpful for understanding the population structure at genetic level and marker-assisted breeding of this vulnerable species.
英文摘要: Fifteen polymorphic microsatellite loci were developed for the Chinese soft-shelled turtle (Pelodiscus sinensis) from the (GT)(n) microsatellite-enriched genomic library, using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol. The polymorphism of all 15 loci ranged from two to seven alleles with observed heterozygosities ranging from 0.03 to 0.98 (mean 0.43) in one population of 40 individuals. These novel loci will be helpful for understanding the population structure at genetic level and marker-assisted breeding of this vulnerable species.
语种: 英语
WOS记录号: WOS:000250647700095
ISSN号: 1471-8278
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8376
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430079, Peoples R China
2.Minist Water Resources, Inst Hydroecol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Wuhan 430072, Peoples R China
4.World Sturgeon Conservat Soc, D-21629 Neu Wulmstorf, Germany
5.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Que, Yanfu; Zhu, Bin; Rosenthal, H.; Chang, Jianbo.Isolation and characterization of microsatellites in Chinese soft-shelled turtle, Pelodiscus sinensis,MOLECULAR ECOLOGY NOTES,2007,7(6):1265-1267
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