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Developmental expression of CagMdkb during gibel carp embryogenesis
Yin, Jun; Xia, Jian-Hong; Du, Xin-Zheng; Liu, Jun; Zhou, Li; Hong, Yun-Han; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
2007
Source PublicationINTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
ISSN0214-6282
Volume51Issue:8Pages:761-769
AbstractMidkine (Mdk) genes have been revealed to have different expression patterns in vertebrates and therefore, additional studies on Mdk expression patterns are required in more species. In this study, CagMdkb has been cloned and characterized from a SMART cDNA library of 10-somite stage embryos of Carassius auratus gibelio. Its full length cDNA is 1091 bp and encodes a sequence of 147 amino acids, which shows 97.3% identity to zebrafish Mdkb on the amino acid level. RT-PCR analysis reveals that CagMdkb is first transcribed in gastrula embryos and maintains a relatively stable expression level during subsequent embryogenesis. Western blot analysis reveals a 19 kDa maternal CagMdkb protein band and the zygotic CagMdkb protein is expressed from gastrula stage. At around 10 somite stage, the 19 kDa CagMdkb is processed to another protein band of about 17 kDa, which might be the secreted form with the 21-residue signal peptide removed. With immunofluorescence analysis, maternal CagMdkb protein was found to be localized in each blastamere cell of early embryos. The zygotic CagMdkb positive fluorescence signal was detected from a pair of large neurons at 18-somite stage. At the later stages, CagMdkb protein was also extended to numerous small neurons in the forebrain, midbrain and hindbrain, as well as to nerve fibers in the spinal cord. Co-localization with 3A10 antibody revealed CagMdkb immunoreactivity on developing Mauthner neurons, a member of reticulospinal neurons. In addition, ectopic expression of CagMdkb in early embryos of gibel carp and zebrafish suppressed head formation and CagMdkb function was found to depend on secretory activity. All these findings indicate that CagMdkb plays an important role in neural development during gibel carp embryogenesis and there is functional conservation of Mdkb in fish head formation.; Midkine (Mdk) genes have been revealed to have different expression patterns in vertebrates and therefore, additional studies on Mdk expression patterns are required in more species. In this study, CagMdkb has been cloned and characterized from a SMART cDNA library of 10-somite stage embryos of Carassius auratus gibelio. Its full length cDNA is 1091 bp and encodes a sequence of 147 amino acids, which shows 97.3% identity to zebrafish Mdkb on the amino acid level. RT-PCR analysis reveals that CagMdkb is first transcribed in gastrula embryos and maintains a relatively stable expression level during subsequent embryogenesis. Western blot analysis reveals a 19 kDa maternal CagMdkb protein band and the zygotic CagMdkb protein is expressed from gastrula stage. At around 10 somite stage, the 19 kDa CagMdkb is processed to another protein band of about 17 kDa, which might be the secreted form with the 21-residue signal peptide removed. With immunofluorescence analysis, maternal CagMdkb protein was found to be localized in each blastamere cell of early embryos. The zygotic CagMdkb positive fluorescence signal was detected from a pair of large neurons at 18-somite stage. At the later stages, CagMdkb protein was also extended to numerous small neurons in the forebrain, midbrain and hindbrain, as well as to nerve fibers in the spinal cord. Co-localization with 3A10 antibody revealed CagMdkb immunoreactivity on developing Mauthner neurons, a member of reticulospinal neurons. In addition, ectopic expression of CagMdkb in early embryos of gibel carp and zebrafish suppressed head formation and CagMdkb function was found to depend on secretory activity. All these findings indicate that CagMdkb plays an important role in neural development during gibel carp embryogenesis and there is functional conservation of Mdkb in fish head formation.
SubtypeArticle
KeywordMdkb Embryogenesis Mauthner Cell Nerve Development Gibel Carp
DepartmentChinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Subject AreaDevelopmental Biology
DOI10.1387/ijdb.072346jy
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000251194200010
WOS KeywordCARASSIUS-AURATUS-GIBELIO ; ACID-RESPONSIVE GENE ; TERATOCARCINOMA SYSTEM ; MOUSE EMBRYOGENESIS ; GROWTH-FACTORS ; MIDKINE ; ZEBRAFISH ; PLEIOTROPHIN ; FISH ; IDENTIFICATION
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8356
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
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Yin, Jun,Xia, Jian-Hong,Du, Xin-Zheng,et al. Developmental expression of CagMdkb during gibel carp embryogenesis[J]. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY,2007,51(8):761-769.
APA Yin, Jun.,Xia, Jian-Hong.,Du, Xin-Zheng.,Liu, Jun.,Zhou, Li.,...&Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China.(2007).Developmental expression of CagMdkb during gibel carp embryogenesis.INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY,51(8),761-769.
MLA Yin, Jun,et al."Developmental expression of CagMdkb during gibel carp embryogenesis".INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 51.8(2007):761-769.
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