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A gene cluster that regulates both heterocyst differentiation and pattern formation in Anabaena sp strain PCC 7120
Zhang, Wei; Du, Ye; Khudyakov, Ivan; Fan, Qing; Gao, Hong; Ning, Degang; Wolk, C. Peter; Xu, Xudong; Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2007-12-01
Source PublicationMOLECULAR MICROBIOLOGY
ISSN0950-382X
Volume66Issue:6Pages:1429-1443
AbstractWild-type Anabaena sp. strain PCC 7120, a filamentous nitrogen-fixing cyanobacterium, produces single heterocysts at semi-regular intervals. asr0100 (patU5) and alr0101 (patU3) are homologous to the 5' and 3' portions of patU of Nostoc punctiforme. alr0099 (hetZ) overlaps the 5' end of patU5. hetZ, patU5 and patU3 were all upregulated, or expressed specifically, in proheterocysts and heterocysts. Mutants of hetZ showed delayed or no heterocyst differentiation. In contrast, a patU3 mutation produced a multiple contiguous heterocyst (Mch) phenotype and restored the formation of otherwise lost intercalary heterocysts in a patA background. Decreasing the expression of patU3 greatly increased the frequency of heterocysts in a mini-patS strain. Two promoter regions and two principal, corresponding transcripts were detected in the hetZ-patU5-patU3 region. Transcription of hetZ was upregulated in a hetZ mutant and downregulated in a patU3 mutant. When mutants hetZ::C.K2 and hetZ::Tn5-1087b were nitrogen-deprived, P-hetC-gfp was very weakly expressed, and in hetZ::Tn5-1087b, P-hetR-gfp was relatively strongly expressed in cells that had neither a regular pattern nor altered morphology. We conclude that the hetZ-patU5-patU3 cluster plays an important role in co-ordination of heterocyst differentiation and pattern formation. The presence of homologous clusters in filamentous genera without heterocysts is suggestive of a more general role.; Wild-type Anabaena sp. strain PCC 7120, a filamentous nitrogen-fixing cyanobacterium, produces single heterocysts at semi-regular intervals. asr0100 (patU5) and alr0101 (patU3) are homologous to the 5' and 3' portions of patU of Nostoc punctiforme. alr0099 (hetZ) overlaps the 5' end of patU5. hetZ, patU5 and patU3 were all upregulated, or expressed specifically, in proheterocysts and heterocysts. Mutants of hetZ showed delayed or no heterocyst differentiation. In contrast, a patU3 mutation produced a multiple contiguous heterocyst (Mch) phenotype and restored the formation of otherwise lost intercalary heterocysts in a patA background. Decreasing the expression of patU3 greatly increased the frequency of heterocysts in a mini-patS strain. Two promoter regions and two principal, corresponding transcripts were detected in the hetZ-patU5-patU3 region. Transcription of hetZ was upregulated in a hetZ mutant and downregulated in a patU3 mutant. When mutants hetZ::C.K2 and hetZ::Tn5-1087b were nitrogen-deprived, P-hetC-gfp was very weakly expressed, and in hetZ::Tn5-1087b, P-hetR-gfp was relatively strongly expressed in cells that had neither a regular pattern nor altered morphology. We conclude that the hetZ-patU5-patU3 cluster plays an important role in co-ordination of heterocyst differentiation and pattern formation. The presence of homologous clusters in filamentous genera without heterocysts is suggestive of a more general role.
SubtypeArticle
KeywordCyanobacterium Anabaena
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; St Petersburg State Univ, All Russia Res Inst Agr Microbiol, St Petersburg, Russia; St Petersburg State Univ, Biol Res Inst, St Petersburg, Russia; Michigan State Univ, MSU DOE Plant Res Lab, E Lansing, MI 48824 USA; Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA
Subject AreaBiochemistry & Molecular Biology ; Microbiology
DOI10.1111/j.1365-2958.2007.05997.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Microbiology
WOS IDWOS:000251252200012
WOS KeywordCYANOBACTERIUM ANABAENA ; NOSTOC-PUNCTIFORME ; RESPONSE REGULATOR ; HETR FUNCTION ; EXPRESSION ; PATS ; NTCA ; DNA ; MAINTENANCE ; NITROGENASE
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Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8354
Collection期刊论文
Corresponding AuthorXu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.St Petersburg State Univ, All Russia Res Inst Agr Microbiol, St Petersburg, Russia
3.St Petersburg State Univ, Biol Res Inst, St Petersburg, Russia
4.Michigan State Univ, MSU DOE Plant Res Lab, E Lansing, MI 48824 USA
5.Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA
Recommended Citation
GB/T 7714
Zhang, Wei,Du, Ye,Khudyakov, Ivan,et al. A gene cluster that regulates both heterocyst differentiation and pattern formation in Anabaena sp strain PCC 7120[J]. MOLECULAR MICROBIOLOGY,2007,66(6):1429-1443.
APA Zhang, Wei.,Du, Ye.,Khudyakov, Ivan.,Fan, Qing.,Gao, Hong.,...&Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.(2007).A gene cluster that regulates both heterocyst differentiation and pattern formation in Anabaena sp strain PCC 7120.MOLECULAR MICROBIOLOGY,66(6),1429-1443.
MLA Zhang, Wei,et al."A gene cluster that regulates both heterocyst differentiation and pattern formation in Anabaena sp strain PCC 7120".MOLECULAR MICROBIOLOGY 66.6(2007):1429-1443.
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