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Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1
Shi, Yan; Zhang, Yi-Bing; Zhao, Zhe; Jiang, Jun; Zhang, Qi-Ya; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume32Issue:2Pages:134-146
AbstractInterferon (IFN)-regulatory transcription factor-1 (IRF-1) has been studied in mammals and fish but little is known about the relationship between its gene structure and nuclear 'ion of IRF-1 protein. In this study, a cDNA encoding Carassius auratus IRF-1 (CaIRF-1) was isolated from an interferon-producing cell line, C. ouratus blastulae embryonic (CAB) cells, exposed to UV-inactivated grass carp hemorrhagic virus (GCHV). The CaIRF-1 genomic locus exhibits exon-intron arrangements similar to those of other vertebrate IRF-1 loci, with nine exons and eight introns, although together with pufferfish IRF-1, CaIRF-1 distinguishes itself from other vertebrate IRF-1 genes by a relatively compact genomic size. Similar to the known IRF-1 genes, CaIRF-1 is ubiquitously expressed, and is upregulated in vitro and in vivo in response to virus, Poty I:C, or CAB INF-containing supernatant (ICS). Subcellular localization analysis confirms the nuclear distribution of CaIRF-1 protein, and reveals two nuclear localization signals (NILS), any one of which is sufficient for nuclear translocation of CaIRF-1. One NLS Locates to amino acids 117-146, and appears to be the structural and functional equivalent of the NLS in mammalian IRF-1. The second NLS (amino acids 73-115) is found within the DNA-binding domain (DBD) of CaIRF-1, and contains two regions rich in basic amino acids (''(KDKSINK101)-K-95" and ''(75)KTWKANFR(82)"). In comparison with mammalian IRF-1, in which the corresponding amino acid stretch does not seem to drive nuclear translocation, five conserved basic amino acids (K-75, K-78, R-82, K-95, and K-101) and one non-conserved basic amino acid (K-97) are present in this NLS from CaIRF-1. This observation suggests that K97 Of CaIRF-1 might be essential for the function of its second NLS, wherein the six basic aminoacids might cooperate to drive CaIRF-1 to the nucleus. Therefore, the current study has revealed a new nuclear localization motif in the DBD of a vertebrate IRF-1. (C) 2007 Elsevier Ltd. All rights reserved.; Interferon (IFN)-regulatory transcription factor-1 (IRF-1) has been studied in mammals and fish but little is known about the relationship between its gene structure and nuclear 'ion of IRF-1 protein. In this study, a cDNA encoding Carassius auratus IRF-1 (CaIRF-1) was isolated from an interferon-producing cell line, C. ouratus blastulae embryonic (CAB) cells, exposed to UV-inactivated grass carp hemorrhagic virus (GCHV). The CaIRF-1 genomic locus exhibits exon-intron arrangements similar to those of other vertebrate IRF-1 loci, with nine exons and eight introns, although together with pufferfish IRF-1, CaIRF-1 distinguishes itself from other vertebrate IRF-1 genes by a relatively compact genomic size. Similar to the known IRF-1 genes, CaIRF-1 is ubiquitously expressed, and is upregulated in vitro and in vivo in response to virus, Poty I:C, or CAB INF-containing supernatant (ICS). Subcellular localization analysis confirms the nuclear distribution of CaIRF-1 protein, and reveals two nuclear localization signals (NILS), any one of which is sufficient for nuclear translocation of CaIRF-1. One NLS Locates to amino acids 117-146, and appears to be the structural and functional equivalent of the NLS in mammalian IRF-1. The second NLS (amino acids 73-115) is found within the DNA-binding domain (DBD) of CaIRF-1, and contains two regions rich in basic amino acids (''(KDKSINK101)-K-95" and ''(75)KTWKANFR(82)"). In comparison with mammalian IRF-1, in which the corresponding amino acid stretch does not seem to drive nuclear translocation, five conserved basic amino acids (K-75, K-78, R-82, K-95, and K-101) and one non-conserved basic amino acid (K-97) are present in this NLS from CaIRF-1. This observation suggests that K97 Of CaIRF-1 might be essential for the function of its second NLS, wherein the six basic aminoacids might cooperate to drive CaIRF-1 to the nucleus. Therefore, the current study has revealed a new nuclear localization motif in the DBD of a vertebrate IRF-1. (C) 2007 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordInterferon Regulator Factor-1 (Irf-1) Carassius Auratus Grass Carp Hemorrhagic Virus Inductive Expression Nuclear Localization Signal (Nls) Subcellular Localization
Department[Shi, Yan; Zhang, Yi-Bing; Zhao, Zhe; Jiang, Jun; Zhang, Qi-Ya; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaImmunology ; Zoology
DOI10.1016/i.dci.2007.05.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000252062400006
WOS KeywordUV-INACTIVATED VIRUS ; DNA-BINDING DOMAIN ; PARALICHTHYS-OLIVACEUS ; TRANSCRIPTION FACTOR ; EXPRESSION ANALYSIS ; GENE-EXPRESSION ; TRANSACTIVATION DOMAIN ; JAPANESE FLOUNDER ; INDUCIBLE GENES ; MESSENGER-RNA
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8322
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Shi, Yan,Zhang, Yi-Bing,Zhao, Zhe,et al. Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2008,32(2):134-146.
APA Shi, Yan.,Zhang, Yi-Bing.,Zhao, Zhe.,Jiang, Jun.,Zhang, Qi-Ya.,...&Gui, JF, Chinese Acad Sci, Grad Sch Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,32(2),134-146.
MLA Shi, Yan,et al."Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 32.2(2008):134-146.
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