[Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Wilhelm, Steven W.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA; [Carmichael, Wayne W.] Wright State Univ, Dept Biol Sci, Dayton, OH 45435 USA; [Watanabe, Makoto M.] Natl Inst Environm Studies, Tsukuba, Ibaraki 3050053, Japan
WOS标题词:
Science & Technology
; Life Sciences & Biomedicine
类目[WOS]:
Marine & Freshwater Biology
研究领域[WOS]:
Marine & Freshwater Biology
摘要:
Two strains of Raphidiopsis Fritsch et Rich were isolated from a fishpond in Wuhan city, China and rendered axenic, and characterized by a combination of morphological, physiological, biochemical and genetic methods. Morphologically the strains were identified as Raphidiopsis mediterranea Skuja (straight trichomes) and R. curvata Fritsch et Rich (coiled trichomes). These two strains demonstrated slight differences in optimal temperature range and GC content, while sharing some common characteristics including inability to grow hetertrophically, similar salinity tolerance (up to 0.78%) and an identical fatty acid composition. Cyanotoxins were not found in the strain of R. mediterranea, however, the strain of R. curvata contained both deoxycylindrospermopsin and cylindrospermopsin. Phylogenetic affiliations inferred from 16S rRNA gene sequences demonstrated that both Raphidiopsis strains clustered with Cylindrospermopsis, demonstrating their phylogenetic ties to Nostocaceae. (c) 2007 Elsevier B.V. All rights reserved.
英文摘要:
Two strains of Raphidiopsis Fritsch et Rich were isolated from a fishpond in Wuhan city, China and rendered axenic, and characterized by a combination of morphological, physiological, biochemical and genetic methods. Morphologically the strains were identified as Raphidiopsis mediterranea Skuja (straight trichomes) and R. curvata Fritsch et Rich (coiled trichomes). These two strains demonstrated slight differences in optimal temperature range and GC content, while sharing some common characteristics including inability to grow hetertrophically, similar salinity tolerance (up to 0.78%) and an identical fatty acid composition. Cyanotoxins were not found in the strain of R. mediterranea, however, the strain of R. curvata contained both deoxycylindrospermopsin and cylindrospermopsin. Phylogenetic affiliations inferred from 16S rRNA gene sequences demonstrated that both Raphidiopsis strains clustered with Cylindrospermopsis, demonstrating their phylogenetic ties to Nostocaceae. (c) 2007 Elsevier B.V. All rights reserved.
1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA 3.Wright State Univ, Dept Biol Sci, Dayton, OH 45435 USA 4.Natl Inst Environm Studies, Tsukuba, Ibaraki 3050053, Japan
Recommended Citation:
Li, Renhui; Wilhelm, Steven W.; Carmichael, Wayne W.; Watanabe, Makoto M..Polyphasic characterization of water bloom forming Raphidiopsis species (cyanobacteria) from central China,HARMFUL ALGAE,2008,7(2):146-153
