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学科主题: Biochemistry & Molecular Biology
题名: Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection
作者: Pei, De-Sheng1, 2; Sun, Yong-Hua1; Zhu, Zuo-Yan1
通讯作者: Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: COXI ; Danio rerio ; Gobiocypris rarus ; MP-PCR ; sybr Green I detection
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2008-03-01
DOI: 10.1007/s11033-006-9050-6
卷: 35, 期:1, 页:45-50
收录类别: SCI
文章类型: Article
部门归属: [Pei, De-Sheng; Sun, Yong-Hua; Zhu, Zuo-Yan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Pei, De-Sheng] Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: To distinguish the cytoplasm of Danio rerio from that of Gobiocypris rarus, we cloned G. rarus COXI and constructed cytoplasmic molecular markers at the high identity domains of COXI by mutated primer PCR (MP-PCR for short). Then Sybr Green I was used to detect the single amplicon. As a result, we succeeded in getting the cytoplasmic molecular markers, G.M COXI and Z.M COXI, by MP-PCR strategy. They were used to detect the sperm-derived mtDNA in the sexual hybrid embryos (D. rerio female x G. rarus male) before the sphere stage. In the present study, all results demonstrate that MP-PCR approach and Sybr Green I detection are feasible to construct the molecular markers to identify genes that shared high identity.
英文摘要: To distinguish the cytoplasm of Danio rerio from that of Gobiocypris rarus, we cloned G. rarus COXI and constructed cytoplasmic molecular markers at the high identity domains of COXI by mutated primer PCR (MP-PCR for short). Then Sybr Green I was used to detect the single amplicon. As a result, we succeeded in getting the cytoplasmic molecular markers, G.M COXI and Z.M COXI, by MP-PCR strategy. They were used to detect the sperm-derived mtDNA in the sexual hybrid embryos (D. rerio female x G. rarus male) before the sphere stage. In the present study, all results demonstrate that MP-PCR approach and Sybr Green I detection are feasible to construct the molecular markers to identify genes that shared high identity.
关键词[WOS]: POLYMERASE CHAIN-REACTION ; CELL NUCLEAR TRANSFER ; MITOCHONDRIAL-DNA ; SOMATIC-CELL ; AMPLIFICATION ; ZEBRAFISH
语种: 英语
WOS记录号: WOS:000252541400007
ISSN号: 0301-4851
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8286
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China

Recommended Citation:
Pei, De-Sheng; Sun, Yong-Hua; Zhu, Zuo-Yan.Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection,MOLECULAR BIOLOGY REPORTS,2008,35(1):45-50
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