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Spatiotemporal heterogeneity of plankton communities in Lake Donghu, China, as revealed by PCR-denaturing gradient gel electrophoresis and its relation to biotic and abiotic factors
Yu, Yuhe1; Yan, Qingyun1,2; Feng, Weisong1; Yu, YH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008-03-01
Source PublicationFEMS MICROBIOLOGY ECOLOGY
ISSN0168-6496
Volume63Issue:3Pages:328-337
AbstractThe 16S and 18S rRNA genes of planktonic organisms derived from five stations with nutrient gradients in Lake Donghu, China, were studied by PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting, and the relationships between the genetic diversity of the plankton community and biotic/abiotic factors are discussed. The concentrations of total nitrogen (TN), total phosphorus (TP), NH4-N and As were found to be significantly related (P < 0.05) to morphological composition of the plankton community. Both chemical and morphological analyses suggested that temporal heterogeneity was comparatively higher than spatial heterogeneity in Lake Donghu. Although the morphological composition was not identical to the DGGE fingerprints in characterizing habitat similarity, the two strongest eutrophic stations (I and II) were always initially grouped into one cluster. Canonical correspondence analysis suggested that the factors strongly correlated with the first two ordination axes were seasonally different. The concentrations of TN and TP and the densities of rotifers and crustaceans were generally the main factors related to the DGGE patterns of the plankton communities. The study suggested that genetic diversity as depicted by metagenomic techniques (such as PCR-DGGE fingerprinting) is a promising tool for ecological study of plankton communities and that such techniques are likely to play an increasingly important role in assessing the environmental conditions of aquatic habitats.; The 16S and 18S rRNA genes of planktonic organisms derived from five stations with nutrient gradients in Lake Donghu, China, were studied by PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting, and the relationships between the genetic diversity of the plankton community and biotic/abiotic factors are discussed. The concentrations of total nitrogen (TN), total phosphorus (TP), NH4-N and As were found to be significantly related (P < 0.05) to morphological composition of the plankton community. Both chemical and morphological analyses suggested that temporal heterogeneity was comparatively higher than spatial heterogeneity in Lake Donghu. Although the morphological composition was not identical to the DGGE fingerprints in characterizing habitat similarity, the two strongest eutrophic stations (I and II) were always initially grouped into one cluster. Canonical correspondence analysis suggested that the factors strongly correlated with the first two ordination axes were seasonally different. The concentrations of TN and TP and the densities of rotifers and crustaceans were generally the main factors related to the DGGE patterns of the plankton communities. The study suggested that genetic diversity as depicted by metagenomic techniques (such as PCR-DGGE fingerprinting) is a promising tool for ecological study of plankton communities and that such techniques are likely to play an increasingly important role in assessing the environmental conditions of aquatic habitats.
SubtypeArticle
KeywordGenetic Diversity Plankton Community Pcr-dgge Species Composition Environmental Factors Lake Donghu
Department[Yu, Yuhe; Yan, Qingyun; Feng, Weisong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Yan, Qingyun] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Subject AreaMicrobiology
DOI10.1111/j.1574-6941.2007.00430.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000252966300007
WOS Keyword16S RIBOSOMAL-RNA ; ENVIRONMENTAL-FACTORS ; MOLECULAR ECOLOGY ; GENETIC DIVERSITY ; TROPHIC STATUS ; WATER-QUALITY ; BACTERIOPLANKTON ; MICROORGANISMS ; POPULATIONS ; PHYTOPLANKTON
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8274
Collection期刊论文
Corresponding AuthorYu, YH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Yu, Yuhe,Yan, Qingyun,Feng, Weisong,et al. Spatiotemporal heterogeneity of plankton communities in Lake Donghu, China, as revealed by PCR-denaturing gradient gel electrophoresis and its relation to biotic and abiotic factors[J]. FEMS MICROBIOLOGY ECOLOGY,2008,63(3):328-337.
APA Yu, Yuhe,Yan, Qingyun,Feng, Weisong,&Yu, YH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).Spatiotemporal heterogeneity of plankton communities in Lake Donghu, China, as revealed by PCR-denaturing gradient gel electrophoresis and its relation to biotic and abiotic factors.FEMS MICROBIOLOGY ECOLOGY,63(3),328-337.
MLA Yu, Yuhe,et al."Spatiotemporal heterogeneity of plankton communities in Lake Donghu, China, as revealed by PCR-denaturing gradient gel electrophoresis and its relation to biotic and abiotic factors".FEMS MICROBIOLOGY ECOLOGY 63.3(2008):328-337.
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