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Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus
Jia Weizhang1,2; Zhou Xiuxia1,2; Huang Rong1,2; Guo Qionglin1; Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2007-12-01
Source PublicationPROGRESS IN NATURAL SCIENCE
ISSN1002-0071
Volume17Issue:12Pages:1425-1435
AbstractBy suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352 amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and gamma-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2 recognition sites. GAS is responsible for the gamma-IFN mediated transcription. One conserved site for NF-kappa B was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-kappa B in accordance with the consensus sequence (GGGRN-NYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5' untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I : C.; By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352 amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and gamma-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2 recognition sites. GAS is responsible for the gamma-IFN mediated transcription. One conserved site for NF-kappa B was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-kappa B in accordance with the consensus sequence (GGGRN-NYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5' untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I : C.
SubtypeArticle
KeywordInterferon Interferon Stimulated Gene (Isg) Viperin Isg15 Snakehead (Channa Argus)
Department[Jia Weizhang; Zhou Xiuxia; Huang Rong; Guo Qionglin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Jia Weizhang; Zhou Xiuxia; Huang Rong] Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China
Subject AreaMultidisciplinary Sciences
WOS HeadingsScience & Technology ; Technology
Indexed BySCI
Language英语
WOS Research AreaMaterials Science ; Science & Technology - Other Topics
WOS SubjectMaterials Science, Multidisciplinary ; Multidisciplinary Sciences
WOS IDWOS:000253164300006
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; MOLECULAR-CLONING ; FUNCTIONAL-CHARACTERIZATION ; RESPONSIVE GENES ; MX-GENE ; FISH ; VIRUS ; EXPRESSION ; INFECTION
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8262
Collection期刊论文
Corresponding AuthorGuo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Jia Weizhang,Zhou Xiuxia,Huang Rong,et al. Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus[J]. PROGRESS IN NATURAL SCIENCE,2007,17(12):1425-1435.
APA Jia Weizhang,Zhou Xiuxia,Huang Rong,Guo Qionglin,&Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2007).Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus.PROGRESS IN NATURAL SCIENCE,17(12),1425-1435.
MLA Jia Weizhang,et al."Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus".PROGRESS IN NATURAL SCIENCE 17.12(2007):1425-1435.
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