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Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells
Zhao, Zhe; Shi, Yan; Ke, Fei; Wei, Sun; Gui, Hanfang; Zhang, Qiya; Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2008-03-01
Source PublicationVIROLOGY
ISSN0042-6822
Volume372Issue:1Pages:118-126
AbstractThymidylate synthase (TS), an essential enzyme in DNA synthesis and repair, plays a key role in the events of cell cycle regulation and tumor formation. Here, an investigation was presented about subcellular location and biological function of viral TS from lymphocystis disease virus from China (LCDV-C) in fish cells. Fluorescence microscopy revealed that LCDV-C TS was predominantly localized in the cytoplasm in fish cells. Cell cycle analysis demonstrated that LCDV-C TS promoted cell cycle progression into S and G2/M phase in the constitutive expressed cells. As a result, the cells have a faster growth rate compared with the control cells as revealed by cell growth curves. For foci assay, the TS-expressed cells gave rise to foci 4-5 weeks after incubation. Microscopic examination of the TS-induced foci revealed multilayered growth and crisscross morphology characteristic of transformed cells. Moreover, LCDV-C TS predisposed the transfected cells to acquire an anchorage-independent phenotype and could grow in 0.3% soft agar. So the data reveal LCDV-C TS is sufficient to induce a transformed phenotype in fish cells in vitro and exhibits its potential ability in cell transformation. To our knowledge, it is the first report on viral TS sequences associated with transforming activity. (C) 2007 Elsevier Inc. All rights reserved.; Thymidylate synthase (TS), an essential enzyme in DNA synthesis and repair, plays a key role in the events of cell cycle regulation and tumor formation. Here, an investigation was presented about subcellular location and biological function of viral TS from lymphocystis disease virus from China (LCDV-C) in fish cells. Fluorescence microscopy revealed that LCDV-C TS was predominantly localized in the cytoplasm in fish cells. Cell cycle analysis demonstrated that LCDV-C TS promoted cell cycle progression into S and G2/M phase in the constitutive expressed cells. As a result, the cells have a faster growth rate compared with the control cells as revealed by cell growth curves. For foci assay, the TS-expressed cells gave rise to foci 4-5 weeks after incubation. Microscopic examination of the TS-induced foci revealed multilayered growth and crisscross morphology characteristic of transformed cells. Moreover, LCDV-C TS predisposed the transfected cells to acquire an anchorage-independent phenotype and could grow in 0.3% soft agar. So the data reveal LCDV-C TS is sufficient to induce a transformed phenotype in fish cells in vitro and exhibits its potential ability in cell transformation. To our knowledge, it is the first report on viral TS sequences associated with transforming activity. (C) 2007 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordThymidylate Synthase Lymphocystis Disease Virus From China (Lcdv-c) Cell Transformation Foci Formation Anchorage-independent Growth
Department[Zhao, Zhe; Shi, Yan; Ke, Fei; Wei, Sun; Gui, Hanfang; Zhang, Qiya] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaVirology
DOI10.1016/j.virol.2007.10.028
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000253325300012
WOS KeywordLYMPHOCYSTIS DISEASE VIRUS ; COMPLETE DNA-SEQUENCE ; GENE-EXPRESSION ; PARALICHTHYS-OLIVACEUS ; FAMILY IRIDOVIRIDAE ; COMPLETE GENOME ; PROPAGATION ; PROTEIN ; ENZYME ; CHINA
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8260
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Zhe,Shi, Yan,Ke, Fei,et al. Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells[J]. VIROLOGY,2008,372(1):118-126.
APA Zhao, Zhe.,Shi, Yan.,Ke, Fei.,Wei, Sun.,Gui, Hanfang.,...&Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2008).Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells.VIROLOGY,372(1),118-126.
MLA Zhao, Zhe,et al."Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells".VIROLOGY 372.1(2008):118-126.
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