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Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus
Lei, Yun1; Chen, Hongyu1; Dai, Heping2; Zeng, Zhaorui1; Lin, Yi1; Zhou, Feimeng3; Pang, Daiwen1; Zeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2008-02-28
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume23Issue:7Pages:1200-1207
AbstractThe paper describes the rapid and label-free detection of the white spot syndrome virus (WSSV) using a surface plasmon resonance (SPR) device based on gold films prepared by electroless plating. The plating condition for obtaining films suitable for SPR measurements was optimized. Gold nanoparticles adsorbed on glass slides were characterized by transmission electron microscopy (TEM). Detection of the WSSV was performed through the binding between WSSV in solution and the anti-WSSV single chain variable fragment (scFv antibody) preimmobilized onto the sensor surface. Morphologies of the as-prepared gold films, gold films modified with self-assembled alkanethiol monolayers, and films covered with antibody were examined using an atomic force microscope (AFM). To demonstrate the viability of the method for real sample analysis, WSSV of different concentrations present in a shrimp hemolymph matrix was determined upon optimizing the surface density of the antibody molecules. The SPR device based on the electroless-plated gold films is capable of detecting concentration of WSSV as low as 2.5 ng/mL in 2% shrimp hemolymph, which is one to two orders of magnitude lower than the level measurable by enzyme-linked immunosorbant assays. (c) 2007 Elsevier B.V. All rights reserved.; The paper describes the rapid and label-free detection of the white spot syndrome virus (WSSV) using a surface plasmon resonance (SPR) device based on gold films prepared by electroless plating. The plating condition for obtaining films suitable for SPR measurements was optimized. Gold nanoparticles adsorbed on glass slides were characterized by transmission electron microscopy (TEM). Detection of the WSSV was performed through the binding between WSSV in solution and the anti-WSSV single chain variable fragment (scFv antibody) preimmobilized onto the sensor surface. Morphologies of the as-prepared gold films, gold films modified with self-assembled alkanethiol monolayers, and films covered with antibody were examined using an atomic force microscope (AFM). To demonstrate the viability of the method for real sample analysis, WSSV of different concentrations present in a shrimp hemolymph matrix was determined upon optimizing the surface density of the antibody molecules. The SPR device based on the electroless-plated gold films is capable of detecting concentration of WSSV as low as 2.5 ng/mL in 2% shrimp hemolymph, which is one to two orders of magnitude lower than the level measurable by enzyme-linked immunosorbant assays. (c) 2007 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordSurface Plasmon Resonance White Spot Syndrome Virus Electroless Plating
Department[Lei, Yun; Chen, Hongyu; Zeng, Zhaorui; Lin, Yi; Pang, Daiwen] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; [Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Feimeng] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA
Subject AreaBiophysics ; Analytical ; Biotechnology & Applied Microbiology ; Electrochemistry ; Chemistry ; Nanoscience & Nanotechnology
DOI10.1016/j.bios.2007.10.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000253620900041
WOS KeywordSELF-ASSEMBLED MONOLAYERS ; PENAEID SHRIMP ; GAS-DETECTION ; AU ; QUANTIFICATION ; NANOPARTICLES ; ADHESION ; BINDING ; OLIGONUCLEOTIDE ; HYBRIDIZATION
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8256
Collection期刊论文
Corresponding AuthorZeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
Affiliation1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA
Recommended Citation
GB/T 7714
Lei, Yun,Chen, Hongyu,Dai, Heping,et al. Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus[J]. BIOSENSORS & BIOELECTRONICS,2008,23(7):1200-1207.
APA Lei, Yun.,Chen, Hongyu.,Dai, Heping.,Zeng, Zhaorui.,Lin, Yi.,...&Zeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.(2008).Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus.BIOSENSORS & BIOELECTRONICS,23(7),1200-1207.
MLA Lei, Yun,et al."Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus".BIOSENSORS & BIOELECTRONICS 23.7(2008):1200-1207.
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