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学科主题: Biophysics; Biotechnology & Applied Microbiology; Chemistry ; Analytical; Electrochemistry; Nanoscience & Nanotechnology
题名: Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus
作者: Lei, Yun1; Chen, Hongyu1; Dai, Heping2; Zeng, Zhaorui1; Lin, Yi1; Zhou, Feimeng3; Pang, Daiwen1
通讯作者: Zeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
关键词: surface plasmon resonance ; white spot syndrome virus ; electroless plating
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2008-02-28
DOI: 10.1016/j.bios.2007.10.004
卷: 23, 期:7, 页:1200-1207
收录类别: SCI
文章类型: Article
部门归属: [Lei, Yun; Chen, Hongyu; Zeng, Zhaorui; Lin, Yi; Pang, Daiwen] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; [Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Feimeng] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
摘要: The paper describes the rapid and label-free detection of the white spot syndrome virus (WSSV) using a surface plasmon resonance (SPR) device based on gold films prepared by electroless plating. The plating condition for obtaining films suitable for SPR measurements was optimized. Gold nanoparticles adsorbed on glass slides were characterized by transmission electron microscopy (TEM). Detection of the WSSV was performed through the binding between WSSV in solution and the anti-WSSV single chain variable fragment (scFv antibody) preimmobilized onto the sensor surface. Morphologies of the as-prepared gold films, gold films modified with self-assembled alkanethiol monolayers, and films covered with antibody were examined using an atomic force microscope (AFM). To demonstrate the viability of the method for real sample analysis, WSSV of different concentrations present in a shrimp hemolymph matrix was determined upon optimizing the surface density of the antibody molecules. The SPR device based on the electroless-plated gold films is capable of detecting concentration of WSSV as low as 2.5 ng/mL in 2% shrimp hemolymph, which is one to two orders of magnitude lower than the level measurable by enzyme-linked immunosorbant assays. (c) 2007 Elsevier B.V. All rights reserved.
英文摘要: The paper describes the rapid and label-free detection of the white spot syndrome virus (WSSV) using a surface plasmon resonance (SPR) device based on gold films prepared by electroless plating. The plating condition for obtaining films suitable for SPR measurements was optimized. Gold nanoparticles adsorbed on glass slides were characterized by transmission electron microscopy (TEM). Detection of the WSSV was performed through the binding between WSSV in solution and the anti-WSSV single chain variable fragment (scFv antibody) preimmobilized onto the sensor surface. Morphologies of the as-prepared gold films, gold films modified with self-assembled alkanethiol monolayers, and films covered with antibody were examined using an atomic force microscope (AFM). To demonstrate the viability of the method for real sample analysis, WSSV of different concentrations present in a shrimp hemolymph matrix was determined upon optimizing the surface density of the antibody molecules. The SPR device based on the electroless-plated gold films is capable of detecting concentration of WSSV as low as 2.5 ng/mL in 2% shrimp hemolymph, which is one to two orders of magnitude lower than the level measurable by enzyme-linked immunosorbant assays. (c) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: SELF-ASSEMBLED MONOLAYERS ; PENAEID SHRIMP ; GAS-DETECTION ; AU ; QUANTIFICATION ; NANOPARTICLES ; ADHESION ; BINDING ; OLIGONUCLEOTIDE ; HYBRIDIZATION
语种: 英语
WOS记录号: WOS:000253620900041
ISSN号: 0956-5663
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8256
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA

Recommended Citation:
Lei, Yun; Chen, Hongyu; Dai, Heping; Zeng, Zhaorui; Lin, Yi; Zhou, Feimeng; Pang, Daiwen.Electroless-plated gold films for sensitive surface plasmon resonance detection of white spot syndrome virus,BIOSENSORS & BIOELECTRONICS,2008,23(7):1200-1207
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